Results 171 to 180 of about 153,238 (212)

Enhanced xylitol production: Expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell

Journal of Bioscience and Bioengineering, 2016
Xylitol has numerous applications in food and pharmaceutical industry, and it can be biosynthesized by microorganisms. In the present study, xdh gene, encoding xylitol dehydrogenase (XDH), was cloned from the genome of Gluconobacter oxydans CGMCC 1.49 and overexpressed in Escherichia coli BL21.
Xiang-Hui, Qi, Jing-Fei, Zhu, Jun-Hua, Yun, Jing, Lin, Yi-Lin, Qi, Qi, Guo, Hong, Xu   +6 more
openaire   +2 more sources

Identification of a Xylitol Dehydrogenase Gene from Kluyveromyces marxianus NBRC1777

Molecular Biotechnology, 2012
Xylitol dehydrogenase (XDH) (EC 1.1.1.9) is one of the key enzymes in the xylose fermentation pathway in yeast and fungi. A xylitol dehydrogenase gene (XYL2) encoding a XDH was cloned from Kluyveromyces marxianus NBRC 1777, and the in vivo function was validated by disruption and complementation analysis. The highest activity of KmXDH could be observed
Li, Lulu, Zhang, Ling, Wang, Dongmei, Gao, Xiaolian, Tamaki, Hisanori, Kumagai, Hidehiko, Hong, Jiong   +6 more
openaire   +2 more sources

Isolation and characterization of two xylitol dehydrogenases from Aspergillus niger

Microbiology, 1994
An NADPH-dependent L-xylulose reductase [xylitol:NADP 4-oxidoreductase; EC 1.1.1.10 (LXDH)] from Aspergillus niger was purified and characterized. It is an octamer with a monomeric molecular mass of 32 kDa, showing high specificity for L-xylulose, xylitol and NADP(H).
Witteveen, C.B.F., Weber, F., Busink, R., Visser, J.   +3 more
openaire   +1 more source

Construction of recombinant Escherichia coli expressing xylitol-4-dehydrogenase and optimization for enhanced L-xylulose biotransformation from xylitol

Bioprocess and Biosystems Engineering, 2021
L-Xylulose is a rare ketopentose which inhibits α-glucosidase and is an indicator of hepatitis or liver cirrhosis. This pentose is also a precursor of other rare sugars such as L-xylose, L-ribose or L-lyxose. Recombinant E. coli expressing xylitol-4-dehydrogenase gene of Pantoea ananatis was constructed.
Mesfin Angaw, Tesfay, Xin, Wen, Yujie, Liu, Huibin, Lin, Linxu, Chen, Jianqiang, Lin, Jianqun, Lin   +6 more
openaire   +2 more sources

Purification of Xylitol Dehydrogenase and Improved Production of Xylitol by Increasing XDH Activity and NADH Supply in Gluconobacter oxydans

Journal of Agricultural and Food Chemistry, 2013
Gluconobacter oxydans is known to be a suitable candidate for producing xylitol from d-arabitol. In this study, the enzyme responsible for reducing d-xylulose to xylitol was purified from G. oxydans NH-10 and characterized as xylitol dehydrogenase.
Jinliang, Zhang, Sha, Li, Hong, Xu, Peng, Zhou, Lujia, Zhang, Pingkai, Ouyang   +5 more
openaire   +2 more sources

Induction of the xylitol dehydrogenase of Pullularia pullulans

Canadian Journal of Microbiology, 1988
Pullularia pullulans, a yeastlike fungus, metabolizes xylitol via an intracellular xylitol dehydrogenase. Formation of the enzyme was induced by D-xylose and inhibited by cycloheximide, suggesting de novo synthesis. The induction study was carried out with noninduced cells of P. pullulans harvested from a glycerol medium. The cells initiated synthesis
Juliet K. Sugai, L. A. Veiga
openaire   +1 more source

Xylose reductase and xylitol dehydrogenase activities of D‐xylose‐xylitol‐fermenting Candida guilliermondii

Journal of Basic Microbiology, 1996
AbstractThe effect of the inoculum age and initial xylose concentration on the activities of xylose reductase (XR) and xylitol dehydrogenase (XD) of Candida guilliermondii FTI 20037 were investigated. Under the experimental conditions used, the XR and XD activities were, respectively. NADPH and NAD or NADP dependent. The NADP/NADPH ratio for XR was not
S. S. Silva, M. Vitolo, A. Pessoa, M. G. A. Felipe   +3 more
openaire   +1 more source

Construction and co-expression of plasmid encoding xylitol dehydrogenase and a cofactor regeneration enzyme for the production of xylitol from d-arabitol

Enzyme and Microbial Technology, 2012
The biotransformation of D-arabitol into xylitol was investigated with focus on the conversion of D-xylulose into xylitol. This critical conversion was accomplished using Escherichia coli to co-express a xylitol dehydrogenase gene from Gluconobacter oxydans and a cofactor regeneration enzyme gene which was a glucose dehydrogenase gene from Bacillus ...
Peng, Zhou, Sha, Li, Hong, Xu, Xiaohai, Feng, Pingkai, Ouyang   +4 more
openaire   +2 more sources

Cloning and characterization of a thermostable xylitol dehydrogenase from Rhizobium etli CFN42

Applied Microbiology and Biotechnology, 2010
An NAD(+)-dependent xylitol dehydrogenase from Rhizobium etli CFN42 (ReXDH) was cloned and overexpressed in Escherichia coli. The DNA sequence analysis revealed an open reading frame of 1,044 bp, capable of encoding a polypeptide of 347 amino acid residues with a calculated molecular mass of 35,858 Da.
Manish Kumar, Tiwari, Hee-Jung, Moon, Marimuthu, Jeya, Jung-Kul, Lee   +3 more
openaire   +2 more sources

Cloning and characterization of thermotolerant xylitol dehydrogenases from yeast Pichia angusta

Applied Microbiology and Biotechnology, 2010
Pichia angusta (syn. Hansenula polymorpha) represents one of the rare yeast that can grow and ferment both xylose and glucose at higher temperature (50°C). However, little is known about the enzymes involved in xylose utilization from this species. Previous studies indicated the presence of one xylose reductase and two xylitol dehydrogenase genes in P.
Dipanwita, Biswas, Manish, Datt, Kaliannan, Ganesan, Alok K, Mondal   +3 more
openaire   +2 more sources