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Purification and Properties of Xylitol Dehydrogenase in Pachysolen tannophilus

Purification and Properties of Xylitol Dehydrogenase in Pachysolen tannophilus
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Enhancement of xylitol productivity and yield using a xylitol dehydrogenase gene-disrupted mutant of Candida tropicalis under fully aerobic conditions

Biotechnology Letters, 2006
The effects of glycerol and the oxygen transfer rate on the xylitol production rate by a xylitol dehydrogenase gene (XYL2)-disrupted mutant of Candida tropicalis were investigated.
Jung Hoe Kim
exaly   +2 more sources

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Characterization of xylitol dehydrogenase from debaryomyces hansenii

Applied Biochemistry and Biotechnology, 1996
The xylitol dehydrogenase (EC 1.1.1.9) from xylose-grown cells ofDebaryomyces hansenii was partially purified in two Chromatographic steps, and characterization studies were carried out in order to inves tigate the role of the xylitol dehydrogenase-catalyzed step in the regu lation of D-xylose metabolism.
Francisco Gírio
exaly   +2 more sources

Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis

Biotechnology Letters, 2011
To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed ...
Ko, BS Ko, Byoung-Sam, Kim, DM Kim, Dong-Min, Yoon, BH Yoon, Byoung-Hoon, Bai, S Bai, Suk, Lee, HY Lee, Hyeon-Yong, Kim, JH Kim, Jung-Hoe, Kim, IC Kim, Il-Chul   +6 more
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The Activity of Xylose Reductase and Xylitol Dehydrogenase in Yeasts

Microbiology, 2003
The activity and the cofactor specificity of xylose reductase and xylitol dehydrogenase were studied in extracts of yeasts from the genera Candida, Kluyveromyces, Pachysolen, Pichia, and Torulopsis grown under microaerobic conditions. It was found that xylitol dehydrogenase in all of the yeast species studied is specific for NAD+; xylose reductase in ...
E N, Iablochkova, O I, Bolotnikova, N P, Mikhaĭlova, N N, Nemova, A I, Ginak   +4 more
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Production of xylitol by expressing xylitol dehydrogenase and alcohol dehydrogenase from Gluconobacter thailandicus and co-biotransformation of whole cells

Bioresource Technology, 2018
In the present study, recombinant strains were constructed for xylitol production by cloning and expressing the novel xylitol dehydrogenase (xdh) and alcohol dehydrogenase (adh) genes in E. coli BL21 (DE3) from Gluconobacter thailandicus CGMCC1.3748.
Huanhuan, Zhang, Junhua, Yun, H, Zabed, Miaomiao, Yang, Guoyan, Zhang, Yilin, Qi, Qi, Guo, Xianghui, Qi   +7 more
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NAD+-dependent xylitol dehydrogenase (xdhA) and l-arabitol-4-dehydrogenase (ladA) deletion mutants of Aspergillus oryzae for improved xylitol production

Biotechnology Letters, 2013
Xylitol dehydrogenase (XDHA) and L-arabitol dehydrogenase (LADA) are two key enzymes in xylan metabolism catalyzing the oxidation of xylitol to D-xylulose and arabitol to L-xylulose, respectively. In Aspergillus oryzae, XDHA and LADA are encoded by xdhA and ladA.
K Takamizawa
exaly   +3 more sources

Utilization of Xylitol Dehydrogenase in a Combined Microbial/Enzymatic Process for Production of Xylitol from D-lucose

Applied Biochemistry and Biotechnology, 2002
The production of xylitol from D-glucose occurs through a three-step process in which D-arabitol and D-xylulose are formed as the first and second intermediate product, respectively, and both are obtained via microbial bioconversion reactions. Catalytic hydrogenation of D-xylulose yields xylitol; however, it is contaminated with D-arabitol.
Gerhard, Mayer, Klaus D, Kulbe, Bernd, Nidetzky   +2 more
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Xylitol production by NAD+-dependent xylitol dehydrogenase (xdhA)- and l-arabitol-4-dehydrogenase (ladA)-disrupted mutants of Aspergillus oryzae

Journal of Bioscience and Bioengineering, 2013
Aspergillus oryzae can metabolize xylan to d-xylose and d-xylose to xylitol. However, accumulation of xylitol is controlled by dehydrogenases, such as xylitol dehydrogenase (XDH) and l-arabitol-4-dehydrogenase (LAD), and fluxed into the pentose phosphate pathway. In A. oryzae, XDH and LAD are encoded by xdhA and ladA, respectively.
Asif, Mahmud, Koji, Hattori, Chen, Hongwen, Noriyuki, Kitamoto, Tohru, Suzuki, Kohei, Nakamura, Kazuhiro, Takamizawa   +6 more
openaire   +2 more sources