Results 121 to 130 of about 4,843 (174)

Determination of the anomeric configuration of D-xylose with D-xylose isomerases

Carbohydrate Research, 1976
Abstract The anomeric configuration of D -xylose, resulting from hydrolysis of β- D xylopyranosides by β- D -xylosidase from Bacillus pumilus , has been determined by an enzymic procedure, based on the stereospecificity of D -xylose isomerases. The initial hydrolysis product is α- D -xylose.
H, Kersters-Hilderson, M, Claeyssens, E, van Doorslaer, C K, de Bruyne   +3 more
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Mechanism of Action of D-Xylose Isomerase

Current Protein & Peptide Science, 2000
The present knowledge on the stereochemical mechanism of action of glucose (or xylose) isomerase, one of the highest tonnage industrial enzymes, is summarized. First we deal shortly with experimental methods applied to study the structure and function of this enzyme: enzyme kinetics, protein engineering, X-ray crystallography, nuclear magnetic and ...
B, Asbóth, G, Náray-Szabó
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Xylose isomerases from Thermotoga

2001
Publisher Summary Typically present in microorganisms that grow on xylose, xylose isomerise (XI) converts xylose to xylulose, which is then phosphorylated and enters the pentose-phosphate pathway. Because it also accepts glucose as substrate, XI is used extensively to isomerize glucose to fructose in the manufacture of high fructose corn syrup (HFCS).
C, Vieille, D, Sriprapundh, R M, Kelly, J G, Zeikus   +3 more
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Structure and mechanism of D-xylose isomerase

Faraday Discussions, 1992
The action of xylose isomerase depends on the presence of two divalent cations. Crystal structure analyses of the free enzyme, and of the enzyme bound to a variety of substrates and inhibitors, have provided models for a number of distinct intermediates along the reaction pathway.
D M, Blow, C A, Collyer, J D, Goldberg, O S, Smart   +3 more
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Reduction of PDC1 expression in S. cerevisiae with xylose isomerase on xylose medium

Bioprocess and Biosystems Engineering, 2011
Ethanol production using hemicelluloses has recently become a focus of many researchers. In order to promote D: -xylose fermentation, we cloned the bacterial xylA gene encoding for xylose isomerase with 434 amino acid residues from Agrobacterium tumefaciens, and successfully expressed it in Saccharomyces cerevisiae, a non-xylose assimilating yeast. The
Dong Min, Kim, Seung-Hyun, Choi, Byung Sam, Ko, Gwon-Young, Jeong, Han-Bit, Jang, Jae-Gun, Han, Kyung-Hwan, Jeong, Hyeon-Yong, Lee, Yonggwan, Won, Il-Chul, Kim   +9 more
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Hyperexpression of Escherichia coli Xylose Isomerase

Biotechnology Progress, 1986
AbstractThe xylose isomerase (xylA) structural gene was cloned under the control of the tac promoter and expressed in a xyl+ E. coli strain. Xylose isomerase accounted for approximately 28% of the total cell protein when this tac‐xylA fusion was induced with isopropylthio β‐D‐galactopyranoside.
C A, Batt, E, O'Neill, S R, Novak, J, Ko, A, Sinskey   +4 more
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Simultaneous bioconversion of glucose and xylose to ethanol by Saccharomyces cerevisiae in the presence of xylose isomerase

Applied Microbiology and Biotechnology, 2000
Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of a glucose/xylose mixture was carried out by Saccharomyces cerevisiae in the presence of xylose isomerase. The SIF of 50 g I(-1) xylose gave an ethanol concentration and metabolic yield of 7.5 g l(-1) and 0.36 g (g xylose consumed)(-1).
P, Chandrakant, V S, Bisaria
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Streptomyces glucose/xylose isomerase has a single active site for glucose and xylose

Biochemical and Biophysical Research Communications, 1989
A kinetic method which allows one to evaluate whether an enzyme acting on two different substrates has one or two active sites was employed to study the active site of glucose isomerase which catalyses the isomerization of both glucose and xylose. The experimental data on the rates of hydrolysis of mixtures of various concentrations of glucose and ...
S M, Gaikwad, H S, Pawar, H G, Vartak, V V, Deshpande   +3 more
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Immunoaffinity purification of glucose/xylose isomerase fromStreptomyces

Applied Biochemistry and Biotechnology, 1991
A procedure was developed to purify glucose/xylose isomerase from cell extract of Streptomyces sp. NCIM 2730 using immunoaffinity chromatography. High-titer polyclonal antibodies were raised in rabbit using electrophoretically homogeneous glucose/xylose isomerase as an antigen.
M, Ghatge, Y, Mawal, S, Gaikwad, V, Deshpande   +3 more
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Overproduction of E. coli xylose isomerase

Biotechnology Letters, 1986
A promoterles DNA fragment containing theE. coli xylose isomerase gene and its ribosome binding site was ligated into a plasmid downstream from the strong λ PL promoter. The plasmid was then used to transformE. coli strains containing a temperature-sensitive λ repressor (cI857).
S. M. Lastick, M. Y. Tucker, V. Mackedonski, K. Grohmann   +3 more
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