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Reduction of PDC1 expression in S. cerevisiae with xylose isomerase on xylose medium
Bioprocess and Biosystems Engineering, 2011Ethanol production using hemicelluloses has recently become a focus of many researchers. In order to promote D: -xylose fermentation, we cloned the bacterial xylA gene encoding for xylose isomerase with 434 amino acid residues from Agrobacterium tumefaciens, and successfully expressed it in Saccharomyces cerevisiae, a non-xylose assimilating yeast. The
Dong Min, Kim +9 more
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Hyperexpression of Escherichia coli Xylose Isomerase
Biotechnology Progress, 1986AbstractThe xylose isomerase (xylA) structural gene was cloned under the control of the tac promoter and expressed in a xyl+ E. coli strain. Xylose isomerase accounted for approximately 28% of the total cell protein when this tac‐xylA fusion was induced with isopropylthio β‐D‐galactopyranoside.
C A, Batt +4 more
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Applied Microbiology and Biotechnology, 2000
Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of a glucose/xylose mixture was carried out by Saccharomyces cerevisiae in the presence of xylose isomerase. The SIF of 50 g I(-1) xylose gave an ethanol concentration and metabolic yield of 7.5 g l(-1) and 0.36 g (g xylose consumed)(-1).
P, Chandrakant, V S, Bisaria
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Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of a glucose/xylose mixture was carried out by Saccharomyces cerevisiae in the presence of xylose isomerase. The SIF of 50 g I(-1) xylose gave an ethanol concentration and metabolic yield of 7.5 g l(-1) and 0.36 g (g xylose consumed)(-1).
P, Chandrakant, V S, Bisaria
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Streptomyces glucose/xylose isomerase has a single active site for glucose and xylose
Biochemical and Biophysical Research Communications, 1989A kinetic method which allows one to evaluate whether an enzyme acting on two different substrates has one or two active sites was employed to study the active site of glucose isomerase which catalyses the isomerization of both glucose and xylose. The experimental data on the rates of hydrolysis of mixtures of various concentrations of glucose and ...
S M, Gaikwad +3 more
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Immunoaffinity purification of glucose/xylose isomerase fromStreptomyces
Applied Biochemistry and Biotechnology, 1991A procedure was developed to purify glucose/xylose isomerase from cell extract of Streptomyces sp. NCIM 2730 using immunoaffinity chromatography. High-titer polyclonal antibodies were raised in rabbit using electrophoretically homogeneous glucose/xylose isomerase as an antigen.
M, Ghatge +3 more
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Overproduction of E. coli xylose isomerase
Biotechnology Letters, 1986A promoterles DNA fragment containing theE. coli xylose isomerase gene and its ribosome binding site was ligated into a plasmid downstream from the strong λ PL promoter. The plasmid was then used to transformE. coli strains containing a temperature-sensitive λ repressor (cI857).
S. M. Lastick +3 more
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1966
Publisher Summary This chpater discusses the determination of D-xylose isomerase. The assay method commonly used is based on the determination of the xylulose formed in the reaction by cysteine–carbazole test. One unit is defined as the amount of enzyme required to produce one micromole of D-xylulose in 10 minutes of incubation.
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Publisher Summary This chpater discusses the determination of D-xylose isomerase. The assay method commonly used is based on the determination of the xylulose formed in the reaction by cysteine–carbazole test. One unit is defined as the amount of enzyme required to produce one micromole of D-xylulose in 10 minutes of incubation.
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Paradigm shift in xylose isomerase usage: a novel scenario with distinct applications
Critical Reviews in Biotechnology, 2022Cintia R. Sargo +2 more
exaly
Xylose isomerase from sp. E2 is a promiscuous enzyme with epimerase activity
Enzyme and Microbial Technology, 2023Marcos Silveira Buckeridge +2 more
exaly