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Strategies and applications for alleviating carbon catabolite repression. [PDF]
Lu M +9 more
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Metabolic engineering of Saccharomyces cerevisiae for efficient utilization of pectin-rich biomass. [PDF]
Lee D, Semidey F, Xu L, Oh EJ.
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Xylose Isomerase Catalysed Novel Hexose Epimerization
Biocatalysis and Biotransformation, 1999Novel hexose substrates and new epimerization reactions catalysed by the industrially important D-xylose ketol-isomerase (E.C. 5. 3. 1. 5.) are described. Isomerization equilibrium betweer D-allose and D-psicose or D-sorbose and D-gulose is obtained starting from either of the substrates.
Matti Leisola +2 more
exaly +4 more sources
EUKARYOTIC CELL COMPRISING XYLOSE ISOMERASE
Eukaryotic cell comprising a heterologous xylose isomerase, wherein the xylose isomerase is loaded with Mn and wherein the Mn metal loading is at least 0.10 mol Mn (mol XylA subunit)-1, preferably about 0.30 mol Mn (mol XylA subunit ...
VERHOEVEN MAARTEN D +9 more
core +6 more sources
Mechanism of Action of D-Xylose Isomerase
Current Protein & Peptide Science, 2000The present knowledge on the stereochemical mechanism of action of glucose (or xylose) isomerase, one of the highest tonnage industrial enzymes, is summarized. First we deal shortly with experimental methods applied to study the structure and function of this enzyme: enzyme kinetics, protein engineering, X-ray crystallography, nuclear magnetic and ...
B, Asbóth, G, Náray-Szabó
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Determination of the anomeric configuration of D-xylose with D-xylose isomerases
Carbohydrate Research, 1976Abstract The anomeric configuration of D -xylose, resulting from hydrolysis of β- D xylopyranosides by β- D -xylosidase from Bacillus pumilus , has been determined by an enzymic procedure, based on the stereospecificity of D -xylose isomerases. The initial hydrolysis product is α- D -xylose.
H, Kersters-Hilderson +3 more
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Xylose isomerases from Thermotoga
2001Publisher Summary Typically present in microorganisms that grow on xylose, xylose isomerise (XI) converts xylose to xylulose, which is then phosphorylated and enters the pentose-phosphate pathway. Because it also accepts glucose as substrate, XI is used extensively to isomerize glucose to fructose in the manufacture of high fructose corn syrup (HFCS).
C, Vieille +3 more
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Crystallographic studies of the mechanism of xylose isomerase
Biochemistry, 1989The mechanism of xylose isomerase (EC 5.3.1.5) has been studied with X-ray crystallography. Four refined crystal structures are reported at 3-A resolution: native enzyme, enzyme + glucose, enzyme + glucose + Mg2+, and enzyme + glucose + Mn2+. One of these structures (E.G.Mg) was determined in a crystal mounted in a flow cell.
G K, Farber +4 more
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Structure and mechanism of D-xylose isomerase
Faraday Discussions, 1992The action of xylose isomerase depends on the presence of two divalent cations. Crystal structure analyses of the free enzyme, and of the enzyme bound to a variety of substrates and inhibitors, have provided models for a number of distinct intermediates along the reaction pathway.
D M, Blow +3 more
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Overproduction of d-xylose isomerase in Escherichia coli by cloning the d-xylose isomerase gene
Enzyme and Microbial Technology, 1985Abstract The Escherichia coli d -xylose isomerase ( d -xylose ketol-isomerase, EC 5.3.1.5) gene , xyl A, has been cloned on various E. coli plasmids. However, it has been found that high levels of overproduction of the d -xylose isomerase, the protein product of the xyl A gene, cannot be accomplished by cloning the intact gene on high copy ...
Panagiotis E. Stevis, Nancy W.Y. Ho
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