Results 151 to 160 of about 43,647 (212)

Strategies and applications for alleviating carbon catabolite repression. [PDF]

open access: yesSynth Syst Biotechnol
Lu M   +9 more
europepmc   +1 more source

Xylose Isomerase Catalysed Novel Hexose Epimerization

Biocatalysis and Biotransformation, 1999
Novel hexose substrates and new epimerization reactions catalysed by the industrially important D-xylose ketol-isomerase (E.C. 5. 3. 1. 5.) are described. Isomerization equilibrium betweer D-allose and D-psicose or D-sorbose and D-gulose is obtained starting from either of the substrates.
Matti Leisola   +2 more
exaly   +4 more sources

EUKARYOTIC CELL COMPRISING XYLOSE ISOMERASE

open access: yes, 2018
Eukaryotic cell comprising a heterologous xylose isomerase, wherein the xylose isomerase is loaded with Mn and wherein the Mn metal loading is at least 0.10 mol Mn (mol XylA subunit)-1, preferably about 0.30 mol Mn (mol XylA subunit ...
VERHOEVEN MAARTEN D   +9 more
core   +6 more sources

Mechanism of Action of D-Xylose Isomerase

Current Protein & Peptide Science, 2000
The present knowledge on the stereochemical mechanism of action of glucose (or xylose) isomerase, one of the highest tonnage industrial enzymes, is summarized. First we deal shortly with experimental methods applied to study the structure and function of this enzyme: enzyme kinetics, protein engineering, X-ray crystallography, nuclear magnetic and ...
B, Asbóth, G, Náray-Szabó
openaire   +2 more sources

Determination of the anomeric configuration of D-xylose with D-xylose isomerases

Carbohydrate Research, 1976
Abstract The anomeric configuration of D -xylose, resulting from hydrolysis of β- D xylopyranosides by β- D -xylosidase from Bacillus pumilus , has been determined by an enzymic procedure, based on the stereospecificity of D -xylose isomerases. The initial hydrolysis product is α- D -xylose.
H, Kersters-Hilderson   +3 more
openaire   +2 more sources

Xylose isomerases from Thermotoga

2001
Publisher Summary Typically present in microorganisms that grow on xylose, xylose isomerise (XI) converts xylose to xylulose, which is then phosphorylated and enters the pentose-phosphate pathway. Because it also accepts glucose as substrate, XI is used extensively to isomerize glucose to fructose in the manufacture of high fructose corn syrup (HFCS).
C, Vieille   +3 more
openaire   +2 more sources

Crystallographic studies of the mechanism of xylose isomerase

Biochemistry, 1989
The mechanism of xylose isomerase (EC 5.3.1.5) has been studied with X-ray crystallography. Four refined crystal structures are reported at 3-A resolution: native enzyme, enzyme + glucose, enzyme + glucose + Mg2+, and enzyme + glucose + Mn2+. One of these structures (E.G.Mg) was determined in a crystal mounted in a flow cell.
G K, Farber   +4 more
openaire   +2 more sources

Structure and mechanism of D-xylose isomerase

Faraday Discussions, 1992
The action of xylose isomerase depends on the presence of two divalent cations. Crystal structure analyses of the free enzyme, and of the enzyme bound to a variety of substrates and inhibitors, have provided models for a number of distinct intermediates along the reaction pathway.
D M, Blow   +3 more
openaire   +2 more sources

Overproduction of d-xylose isomerase in Escherichia coli by cloning the d-xylose isomerase gene

Enzyme and Microbial Technology, 1985
Abstract The Escherichia coli d -xylose isomerase ( d -xylose ketol-isomerase, EC 5.3.1.5) gene , xyl A, has been cloned on various E. coli plasmids. However, it has been found that high levels of overproduction of the d -xylose isomerase, the protein product of the xyl A gene, cannot be accomplished by cloning the intact gene on high copy ...
Panagiotis E. Stevis, Nancy W.Y. Ho
openaire   +1 more source

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