Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development. [PDF]
, 2011 Label-free LC-MS/MS-based shot-gun proteomics was used to quantify the differential protein synthesis and metabolite profiling in order to assess metabolic changes during the development of citrus fruits.
Blumwald, Eduardo +8 more
core +3 more sources
The FEBS Journal, EarlyView.The authors identify PbfF (previously annotated as a NAD‐dependent dehydrogenase) as a hitherto unknown enzyme in phosphonate catabolism. Guided by genome context analysis, they show that PbfF is actually a racemase, serving to degrade the natural compound (S)‐2‐amino‐1‐hydroxyethylphosphonate (S‐HAEP).
Francesca Ruffolo +8 more
wiley +1 more source
Shotgun proteomics of Aspergillus niger microsomes upon D-xylose induction [PDF]
Protein secretion plays an eminent role in cell maintenance and adaptation to the extracellular environment of microorganisms. Although protein secretion is an extremely efficient process in filamentous fungi, the mechanisms underlying protein secretion ...
Ferreira de Oliveira, J.M.P. +3 more
core +1 more source
Sweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry [PDF]
, 2008 Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features,
Berggren +26 more
core +3 more sources
Posttranslational modifications of heterologous proteins expressed in Nicotiana benthamiana
Plant Biotechnology Journal, EarlyView.Summary The success of Nicotiana benthamiana as a workhorse for heterologous protein production is closely linked to its accessibility and tolerance to genetic manipulation, allowing efficient engineering of posttranslational protein modifications (PTMs) that are critical for the function and stability of heterologous proteins.
Kathrin Göritzer +2 more
wiley +1 more source
Immobilized Trienzymatic System with Enhanced Stabilization for the Biotransformation of Lactose
Molecules, 2017 The use of ketohexose isomerases is a powerful tool in lactose whey processing, but these enzymes can be very sensitive and expensive. Development of immobilized/stabilized biocatalysts could be a further option to improve the process.
Pedro Torres, Francisco Batista-Viera
doaj +1 more source
Structure of l-rhamnose isomerase in complex with l-rhamnopyranose demonstrates the sugar-ring opening mechanism and the role of a substrate sub-binding site [PDF]
, 2012 l-Rhamnose isomerase (l-RhI) catalyzes the reversible isomerization of l-rhamnose to l-rhamnulose. Previously determined X-ray structures of l-RhI showed a hydride-shift mechanism for the isomerization of substrates in a linear form, but the mechanism ...
Izumori, Ken +5 more
core +1 more source
Food Science &Nutrition, Volume 13, Issue 7, July 2025.This study evaluated melatonin and arginine effects on bioactive compounds in Valencia orange peel during 6 months of cold storage. Mel (1 mM) enhanced fructose, ascorbic, citric, and chlorogenic acids, whereas Arg (10 mM) increased sucrose, ascorbic acid, and phenolic compounds. Both treatments preserved and enhanced bioactive compounds in orange peel.
Faezeh Aghaei +4 more
wiley +1 more source
Metabolism of Toxic Sugars by Strains of the Bee Gut Symbiont Gilliamella apicola. [PDF]
, 2016 Social bees collect carbohydrate-rich food to support their colonies, and yet, certain carbohydrates present in their diet or produced through the breakdown of pollen are toxic to bees.
Engel, P. +6 more
core +2 more sources
Involvement of the C terminus in intramolecular nitrogen channeling in glucosamine 6-phosphate synthase: evidence from a 1.6 å crystal structure of the isomerase domain [PDF]
, 1998 Background: Glucosamine 6-phosphate synthase (GlmS) catalyses the first step in hexosamine metabolism, converting fructose-6P (6 phosphate) into glucosamine-6P using glutamine as a nitrogen source.
Badet, Bernard +4 more
core +2 more sources