Метаболическая инженерия дрожжей Hansenula polymorpha для создания эффективных продуцентов этанола [PDF]
До недавнего времени никто не рассматривал метилотрофные дрожжи как потенциальный продуцент биотоплива, в частности биоэтанола, из гидролизатов лигноцеллюлозы.
Дмитрук, К.В. +1 more
core +1 more source
Structure of 3,4-Dihydroxy-2-butanone 4-Phosphate Synthase from Methanococcus jannaschii in Complex with Divalent Metal Ions and the Substrate Ribulose 5-Phosphate: implications for the catalytic mechanism [PDF]
Skeletal rearrangements of carbohydrates are crucial for many biosynthetic pathways. In riboflavin biosynthesis ribulose 5-phosphate is converted into 3,4-dihydroxy-2-butanone 4-phosphate while its C4 atom is released as formate in a sequence of metal ...
Bacher, Adelbert +5 more
core
Sn-Beta zeolites with borate salts catalyse the epimerization of carbohydrates via an intramolecular carbon shift [PDF]
Carbohydrate epimerization is an essential technology for the widespread production of rare sugars. In contrast to other enzymes, most epimerases are only active on sugars substituted with phosphate or nucleotide groups, thus drastically restricting ...
A Corma +45 more
core +1 more source
Evolvability of Chaperonin Substrate Proteins [PDF]
Molecular chaperones ensure that their substrate proteins reach the functional native state, and prevent their aggregation. Recently, an additional function was proposed for molecular chaperones: they serve as buffers (_capacitors_) for evolution by ...
Emanuele Raineri +3 more
core +1 more source
Shotgun proteomics of Aspergillus niger microsomes upon D-xylose induction [PDF]
Protein secretion plays an eminent role in cell maintenance and adaptation to the extracellular environment of microorganisms. Although protein secretion is an extremely efficient process in filamentous fungi, the mechanisms underlying protein secretion ...
Ferreira de Oliveira, J.M.P. +3 more
core +1 more source
Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose
To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE).
Pavel Dvořák +11 more
doaj +1 more source
Genome-Resolved Proteomic Stable Isotope Probing of Soil Microbial Communities Using 13CO2 and 13C-Methanol. [PDF]
Stable isotope probing (SIP) enables tracking the nutrient flows from isotopically labeled substrates to specific microorganisms in microbial communities. In proteomic SIP, labeled proteins synthesized by the microbial consumers of labeled substrates are
Banfield, Jillian F +10 more
core +1 more source
Integrated approach for selecting efficient Saccharomyces cerevisiae for industrial lignocellulosic fermentations: importance of yeast chassis linked to process conditions [PDF]
In this work, four robust yeast chassis isolated from industrial environments were engineered with the same xylose metabolic pathway. The recombinant strains were physiologically characterized in synthetic xylose and xylose-glucose medium, on non ...
Costa, Carlos E. +4 more
core +1 more source
Utilization of xylose by engineered strains of Ashbya gossypii for the production of microbial oils [PDF]
BACKGROUND: Ashbya gossypii is a filamentous fungus that is currently exploited for the industrial production of riboflavin. The utilization of A. gossypii as a microbial biocatalyst is further supported by its ability to grow in low-cost feedstocks ...
Alberto Jiménez +4 more
core +1 more source
A Kluyveromyces marxianus 2-deoxyglucose-resistant mutant with enhanced activity of xylose utilization [PDF]
Thermotolerant ethanologenic yeast Kluyveromyces marxianus is capable of fermenting various sugars including xylose but glucose represses to hamper the utilization of other sugars.
Mamoru Yamada +6 more
core +1 more source

