Results 61 to 70 of about 4,838 (170)

Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology

Scientifica, 2018
Glucose isomerase is an enzyme widely used in food industry for producing high-fructose corn syrup. Many microbes, including Bacillus megaterium, have been found to be able to produce glucose isomerase. However, the number of studies of glucose isomerase
Hoang-Yen Thi Nguyen, Gia-Buu Tran
doaj   +1 more source

Reassessing Transketolase Assays: Methodological Considerations for Detecting Functional Thiamine Deficiency

Annals of the New York Academy of Sciences, Volume 1557, Issue 1, March 2026.
Transketolase (TKT) activity provides a functional measure of thiamine status by integrating cofactor availability, enzyme abundance, and catalytic capacity. TKT catalyzes thiamine diphosphate (TDP)‐dependent reactions in the nonoxidative pentose‐phosphate pathway and requires magnesium for optimal activity.
Katie A. Edwards
wiley   +1 more source

Chemical Profiling, In Silico and In Vitro Studies to Identify Potential CDK2 and mTOR Inhibitor From Selaginella inaequalifolia (Hook. & Grev.) Spring Ethanolic Extracts

Chemistry &Biodiversity, Volume 23, Issue 2, February 2026.
ABSTRACT The current study is aimed to reveal the phytoprofile of Selaginella inaequalifolia (Hook. & Grev.) Spring using GC–MS and predict the drug properties, toxicity, biological properties of S. inaequalifolia ethanolic extracts (SiEE) using in silico methods and in vitro toxicity assays, namely, MTT and BSLB assay.
Johnson Marimuthu Alias Antonysamy, Sivaraman Arumugam, Robin Charles Antony Arulraj, Anne Wincy Jacob Thomas, Henrique D. M. Coutinho   +4 more
wiley   +1 more source

Contribution of a Novel TetR/AcrR Family Transcriptional Regulator RalT of Ralstonia pseudosolanacearum Strain OE1‐1 to the Fine‐Tuning of Its Virulence

MicrobiologyOpen, Volume 15, Issue 1, February 2026.
Predicted regulation of quorum sensing‐, ralfuranone‐, and RalT‐dependent genes (QS/Ral/p0599‐dependent genes) via a novel TetR/AcrR family transcriptional regulator RalT in Ralstonia pseudosolanacearum strain OE1‐1 was shown here. The regulation of ralT is dependent on ralfuranone, and contribution of RalT is popular with the regulation of some quorum
Tatsuya Ueyama, Masayuki Tsuzuki, Sora Tateda, Yuki Terazawa, Aoi Ikeuchi, Akinori Kiba, Kouhei Ohnishi, Yasufumi Hikichi   +7 more
wiley   +1 more source

Genome Analysis Reveals Diversity and Functional Potential of Novel Janthinobacterium Species From Subarctic Soils

MicrobiologyOpen, Volume 15, Issue 1, February 2026.
The Pseudomonadota are abundant and diverse members of high‐latitude soils. Here, we describe two novel species, Janthinobacterium silvisoli and Janthinobacterium saanense, of the order Burkholderiales, isolated from tundra heath and northern boreal forest soils.
Anil Kumar, Minna K. Männistö, Lee J. Kerkhof, Max M. Häggblom   +3 more
wiley   +1 more source

The role of a xylose isomerase pathway in the conversion of xylose to lipid in Mucor circinelloides

RSC Advances, 2016
The xylose isomerase (XI) pathway, which converts xylose in lignocellulosic materials into intermediate metabolites, is characterized for the first time in M. circinelloides.
Linfang Chu, Xinyi Zan, Xin Tang, Lina Zhao, Haiqin Chen, Yong Q. Chen, Wei Chen, Yuanda Song   +7 more
openaire   +1 more source

Development of a Tool for High‐Efficiency, Markerless and Iterative Genome Editing in Shouchella clausii

Microbial Biotechnology, Volume 19, Issue 2, February 2026.
Shouchella clausii, a spore‐forming Gram‐positive bacterium with a long‐standing use as probiotic, has long been considered genetically intractable. This study introduces a high‐efficiency electroporation protocol and a versatile Golden Gate‐compatible shuttle vector (pM4B522), enabling precise, scarless and iterative genome editing in both S.
Claudia Cappella, Carsten Jers, Lorenzo Ninivaggi, Maurizio Bettiga, Ivan Mijakovic, Gennaro Agrimi, Pasquale Scarcia   +6 more
wiley   +1 more source

Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose

Nature Communications
To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE).
Pavel Dvořák, Barbora Burýšková, Barbora Popelářová, Birgitta E. Ebert, Tibor Botka, Dalimil Bujdoš, Alberto Sánchez-Pascuala, Hannah Schöttler, Heiko Hayen, Víctor de Lorenzo, Lars M. Blank, Martin Benešík   +11 more
doaj   +1 more source

Heterogeneous Catalytic Transformations of Saccharides by Sn Lewis Acid: Recent Progress

ChemCatChem, Volume 18, Issue 2, 28 January 2026.
The review addresses recent advances in heterogeneous catalytic transformations of lignocellulosic saccharides by tin‐containing materials. ABSTRACT Carbohydrates have been considered as important renewable substrates which are readily available in large quantities from waste streams.
Irina Delidovich
wiley   +1 more source

Structural and Anti‐Inflammatory Activity of Ultrasonic Treating Polysaccharide Extracted From Tremella fuciformis Stem Byproducts

Food Frontiers, Volume 7, Issue 1, January 2026.
This illustration shows the extraction and purification of TFP‐1 from Tremella fuciformis stem byproducts. It highlights TFP‐1's anti‐inflammatory effects by demonstrating its ability to inhibit the NF‐kB complex and decrease the levels of pro‐inflammatory cytokines TNF‐α, IL‐1β, and IL‐6. ABSTRACT This study investigated the structural characteristics
Luqiang Huang, Liting Wu, Lan Zhao, Kaiying Chen, Ruojin Lin, Li Li, Yan Liu, Huibin Chen   +7 more
wiley   +1 more source