Results 61 to 70 of about 4,843 (174)

Systematic Metabolic Engineering and Model‐Guided Optimization for High‐Level Production of L‐Theanine from Xylose in Escherichia coli

Advanced Science, Volume 13, Issue 17, 23 March 2026.
This study is pioneering in constructing the shortest known synthetic pathway for L‐theanine production from xylose within E coli. Through comprehensive metabolic engineering strategies, our engineered strain achieved the highest reported L‐theanine titer from xylose, with a titer of 95.42 g/L, and a yield of 0.55 g/g.
Haolin Han, Boyuan Xue, Guangqi Shan, Meng Meng, Shaojie Wang, Haijia Su   +5 more
wiley   +1 more source

Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose

Nature Communications
To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE).
Pavel Dvořák, Barbora Burýšková, Barbora Popelářová, Birgitta E. Ebert, Tibor Botka, Dalimil Bujdoš, Alberto Sánchez-Pascuala, Hannah Schöttler, Heiko Hayen, Víctor de Lorenzo, Lars M. Blank, Martin Benešík   +11 more
doaj   +1 more source

Biomanufacturing and Scale‐Up: Pathways to Biochemicals, Biofuels, and Biomaterials

Microbial Biotechnology, Volume 19, Issue 8, August 2026.
Carolina A. Barcelos, Pavel Dvořák, Charles J. Foster, Juan Nogales, Juan L. Ramos, Shamlan M. S. Reshamwala, Davinia Salvachúa   +6 more
wiley   +1 more source

Neutron Crystallography Study of Host–Pathogen Recognition Enhanced by Hydrogen/Deuterium Exchange on Carbohydrates

ChemistryEurope, Volume 4, Issue 3, March 2026.
Neutron macromolecular crystallography enabled by deuteration of biomolecules reveals how the opportunistic pathogen Pseudomonas aeruginosa utilizes calcium‐dependent lectin for binding to human sugars. Neutron diffraction on the complex highlights hydrogen bond network, protonation of amino acids and role of calcium ion.
Theodore Arnaud, Chloé Tatol, Juliette M. Devos, Lukas Gajdos, Sébastien Vidal, Marek Korsak, Annabelle Varrot, Michaela Wimmerova, Sophie Feuillastre, Matthew P. Blakeley, Anne Imberty   +10 more
wiley   +1 more source

Reassessing Transketolase Assays: Methodological Considerations for Detecting Functional Thiamine Deficiency

Annals of the New York Academy of Sciences, Volume 1557, Issue 1, March 2026.
Transketolase (TKT) activity provides a functional measure of thiamine status by integrating cofactor availability, enzyme abundance, and catalytic capacity. TKT catalyzes thiamine diphosphate (TDP)‐dependent reactions in the nonoxidative pentose‐phosphate pathway and requires magnesium for optimal activity.
Katie A. Edwards
wiley   +1 more source

Chemical Profiling, In Silico and In Vitro Studies to Identify Potential CDK2 and mTOR Inhibitor From Selaginella inaequalifolia (Hook. & Grev.) Spring Ethanolic Extracts

Chemistry &Biodiversity, Volume 23, Issue 2, February 2026.
ABSTRACT The current study is aimed to reveal the phytoprofile of Selaginella inaequalifolia (Hook. & Grev.) Spring using GC–MS and predict the drug properties, toxicity, biological properties of S. inaequalifolia ethanolic extracts (SiEE) using in silico methods and in vitro toxicity assays, namely, MTT and BSLB assay.
Johnson Marimuthu Alias Antonysamy, Sivaraman Arumugam, Robin Charles Antony Arulraj, Anne Wincy Jacob Thomas, Henrique D. M. Coutinho   +4 more
wiley   +1 more source

Xylose Isomerase Depletion Enhances Virulence of Xanthomonas citri subsp. citri in Citrus aurantifolia. [PDF]

Int J Mol Sci, 2023
Alexandrino AV, Prieto EL, Nicolela NCS, da Silva Marin TG, Dos Santos TA, de Oliveira da Silva JPM, da Cunha AF, Behlau F, Novo-Mansur MTM.   +8 more
europepmc   +1 more source

Development of Efficient Xylose Fermentation in Saccharomyces cerevisiae: Xylose Isomerase as a Key Component

, 2007
Metabolic engineering of Saccharomyces cerevisiae for ethanol production from D-xylose, an abundant sugar in plant biomass hydrolysates, has been pursued vigorously for the past 15 years. Whereas wild-type S. cerevisiae cannot ferment D-xylose, the keto-isomer D-xylulose can be metabolised slowly.
Van Maris, A.J.A. (author), Winkler, A.A. (author), Kuyper, M. (author), De Laat, W.T. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)   +5 more
openaire   +4 more sources

Heterologous expression of Spathaspora passalidarum xylose reductase and xylitol dehydrogenase genes improved xylose fermentation ability of Aureobasidium pullulans

Microbial Cell Factories, 2018
Background Aureobasidium pullulans is a yeast-like fungus that can ferment xylose to generate high-value-added products, such as pullulan, heavy oil, and melanin.
Jian Guo, Siyao Huang, Yefu Chen, Xuewu Guo, Dongguang Xiao   +4 more
doaj   +1 more source

Comparative xylose metabolism among the Ascomycetes C. albicans, S. stipitis and S. cerevisiae.

PLoS ONE, 2013
The ascomycetes Candida albicans, Saccharomyces cerevisiae and Scheffersomyces stipitis metabolize the pentose sugar xylose very differently. S. cerevisiae fails to grow on xylose, while C. albicans can grow, and S.
Doreen Harcus, Daniel Dignard, Guylaine Lépine, Chris Askew, Martine Raymond, Malcolm Whiteway, Cunle Wu   +6 more
doaj   +1 more source