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Sugarcane Biorefinery from Component Separation to High-Value Outputs: Technical Progress and Future Perspectives. [PDF]

Foods
Dai J, Chen J, Lin B, Lu L, Zheng F, Verma KK, Chen G.   +6 more
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Repression of xylose utilization by glucose in xylose-fermenting yeasts

Canadian Journal of Microbiology, 1988
The xylose-fermenting yeasts Pichia stipitis, Candida steatolytica, and Candida shehatae were subjected to fermentations in synthetic media containing mixtures of glucose and xylose. In all cases, repression of xylose uptake by glucose was observed, although the extent of repression was different with each yeast.
Chandra J. Panchal, Lynda Bast, Inge Russell, Graham G. Stewart   +3 more
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Arabinose utilization by xylose-fermenting yeasts and fungi

Applied Biochemistry and Biotechnology, 1994
Various wild-type yeasts and fungi were screened to evaluate their ability to ferment L-arabinose under oxygen-limited conditions when grown in defined minimal media containing mixtures of L-arabinose, D-xylose, and D-glucose. Although all of the yeasts and some of the fungi consumed arabinose, arabinose was not fermented to ethanol by any of the ...
J D, McMillan, B L, Boynton
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Directed evolution of xylose specific transporters to facilitate glucose‐xylose co‐utilization

Biotechnology and Bioengineering, 2015
ABSTRACTA highly active xylose specific transporter without glucose inhibition is highly desirable in cost‐effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a directed evolution strategy to
Meng, Wang, Chenzhao, Yu, Huimin, Zhao
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Effect of glucose on xylose utilization in Saccharomyces cerevisiae harboring the xylose reductase gene

Archives of Microbiology, 2011
We have constructed recombinant Saccharomyces cerevisiae JH1 harboring a xylose reductase gene (xyl1) isolated from Pichia stipitis. However, JH1 still utilizes glucose more easily than xylose. Therefore, in this study, we characterized the effect of a glucose supplement on xylose utilization, the expression level of xylose reductase as a recombinant ...
Ji-Hye, Han, Ju-Yong, Park, Kye Sang, Yoo, Hyun Woo, Kang, Gi-Wook, Choi, Bong-Woo, Chung, Jiho, Min   +6 more
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Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose improves aerobic xylose consumption

Applied Microbiology and Biotechnology, 2008
The goal of this investigation was to determine the effect of a xylose transport system on glucose and xylose co-consumption as well as total xylose consumption in Saccharomyces cerevisiae. We expressed two heterologous transporters from Arabidopsis thaliana in recombinant xylose-utilizing S. cerevisiae cells.
Ronald E, Hector, Nasib, Qureshi, Stephen R, Hughes, Michael A, Cotta   +3 more
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Deletion of d-ribulose-5-phosphate 3-epimerase (RPE1) induces simultaneous utilization of xylose and glucose in xylose-utilizing Saccharomyces cerevisiae

Biotechnology Letters, 2014
Simultaneous co-utilization of xylose and glucose is a key issue in engineering microbes for cellulosic ethanol production. We coupled xylose utilization with glucose metabolism by deletion of D-ribulose-5-phosphate 3-epimerase (RPE1) through pentose phosphate pathway flux.
Hao Song, Bing-Zhi Li, Ying-Jin Yuan
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Metabolic Engineering of Saccharomyces cerevisiae for Xylose Utilization

2001
Metabolic engineering of Saccharomyces cerevisiae for ethanolic fermentation of xylose is summarized with emphasis on progress made during the last decade. Advances in xylose transport, initial xylose metabolism, selection of host strains, transformation and classical breeding techniques applied to industrial polyploid strains as well as modeling of ...
B, Hahn-Hägerdal, C F, Wahlbom, M, Gárdonyi, W H, van Zyl, R R, Cordero Otero, L J, Jönsson   +5 more
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