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Positive selection vectors based on xylose utilization suppression
Gene, 1987High levels of xylose isomerase activity in wild-type Escherichia coli strains results in a Xyl- phenotype. This phenomenon was exploited for the development of a versatile positive selection system. The xylA promoter was deleted with the exonuclease BAL 31 and the resulting structural gene was inserted into the SmaI site of pUC9, yielding the ...
P E, Stevis, N W, Ho
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A method for determination of xylose utilizing glucose dehydrogenase
Clinical Biochemistry, 1994A method is described for xylose determination on the Ciba-Corning 550 Express that utilizes the slower enzymatic action of glucose dehydrogenase (GDH) on D-xylose, after prior removal of glucose. Glucose oxidase is added to serum or urine and incubated for 120 min at 37 degrees C.
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Isolation and characterization of xylose- and xylan-utilizing anaerobic bacteria
Antonie van Leeuwenhoek, 1989By enrichment with xylose, nine mesophilic strains of anaerobic bacteria were obtained from various sources. Two isolates appear to belong to the genus Eubacterium. Six other strains belong to the genus Clostridium. Three of the isolated strains utilized larch wood xylan.
M V, Murty, T S, Chandra
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Utilization of xylose by bacteria, yeasts, and fungi
1983Hemicellulosic sugars, especially d-xylose, are relatively abundant in agricultural and forestry residues. Moreover, they can be recovered from the hemicelluloses by acid hydrolysis more readily and in better yields than can d-glucose from cellulose. These factors favor hemicellulosic sugars as a feedstock for production of ethanol and other chemicals.
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Applied Microbiology and Biotechnology, 2009
During growth of Saccharomyces cerevisiae on glucose, the redox cofactors NADH and NADPH are predominantly involved in catabolism and biosynthesis, respectively. A deviation from the optimal level of these cofactors often results in major changes in the substrate uptake and biomass formation.
Jin, Hou +3 more
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During growth of Saccharomyces cerevisiae on glucose, the redox cofactors NADH and NADPH are predominantly involved in catabolism and biosynthesis, respectively. A deviation from the optimal level of these cofactors often results in major changes in the substrate uptake and biomass formation.
Jin, Hou +3 more
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Utilization of Xylose for Growth by the Eukaryotic Alga, Chlorella
Current Microbiology, 1999A green alga, Chlorella, was found to be capable of utilizing xylose or other pentose sugars (xylitol, arabinose) for enhanced growth rates when grown in the light, but not when grown heterotrophically in the dark. With selection for growth in xylose-containing medium, it was possible to improve dramatically the ability of selected Chlorella strains to
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Applied Microbiology and Biotechnology, 2009
Previously, a Saccharomyces cerevisiae strain was engineered for xylose assimilation by the constitutive overexpression of the Orpinomyces xylose isomerase, the S. cerevisiae xylulokinase, and the Pichia stipitis SUT1 sugar transporter genes. The recombinant strain exhibited growth on xylose, under aerobic conditions, with a specific growth rate of 0 ...
Anjali, Madhavan +5 more
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Previously, a Saccharomyces cerevisiae strain was engineered for xylose assimilation by the constitutive overexpression of the Orpinomyces xylose isomerase, the S. cerevisiae xylulokinase, and the Pichia stipitis SUT1 sugar transporter genes. The recombinant strain exhibited growth on xylose, under aerobic conditions, with a specific growth rate of 0 ...
Anjali, Madhavan +5 more
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Applied Microbiology and Biotechnology, 2007
In the present study, we modified xylose uptake properties of a recombinant xylose-utilizing yeast Saccharomyces cerevisiae by expression of heterologous and homologous permease-encoding genes. In a mutant yeast strain with the main seven hexose transporter genes deleted, and engineered for xylose utilization, we screened an expression cDNA library of ...
Saloheimo, Anu +5 more
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In the present study, we modified xylose uptake properties of a recombinant xylose-utilizing yeast Saccharomyces cerevisiae by expression of heterologous and homologous permease-encoding genes. In a mutant yeast strain with the main seven hexose transporter genes deleted, and engineered for xylose utilization, we screened an expression cDNA library of ...
Saloheimo, Anu +5 more
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Evolutionary adaptation of recombinant shochu yeast for improved xylose utilization
Journal of Bioscience and Bioengineering, 2010We examined the evolutionary adaptation of recombinant shochu yeast by serial anaerobic cultivation in xylose-based minimal medium. Compared with the parental strain, the adapted strain MA-S4-M1 (M1) markedly improved the growth on xylose and the anaerobic xylose consumption rate.
Akinori Matsushika, Shigeki Sawayama
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Xylose utilization in ethanol production: a patent landscape
Biofuels, Bioproducts and Biorefining, 2016Bioethanol is considered as one of the best alternatives to fossil fuels as unwanted biomass is converted into fuel ethanol. As there is increasing demand for fuel ethanol, researchers have started looking for some better ways to produce ethanol. In light of the present scenario, we have attempted to develop a global patent landscape view of xylose ...
Devarapalli, Pratap +2 more
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