Results 51 to 60 of about 1,109,073 (341)

Treatment with surfactants enables quantification of translational activity by O-propargyl-puromycin labelling in yeast

BMC Microbiology, 2021
Background Translation is an important point of regulation in protein synthesis. However, there is a limited number of methods available to measure global translation activity in yeast. Recently, O-propargyl-puromycin (OPP) labelling has been established
Jennifer Staudacher, Corinna Rebnegger, Brigitte Gasser   +2 more
doaj   +1 more source

Specific Functional Features of the Cell Integrity MAP Kinase Pathway in the Dimorphic Fission Yeast Schizosaccharomyces japonicus

Journal of Fungi, 2021
Mitogen activated protein kinase (MAPK) signaling pathways execute essential functions in eukaryotic organisms by transducing extracellular stimuli into adaptive cellular responses.
Elisa Gómez-Gil, Alejandro Franco, Beatriz Vázquez-Marín, Francisco Prieto-Ruiz, Armando Pérez-Díaz, Jero Vicente-Soler, Marisa Madrid, Teresa Soto, José Cansado   +8 more
doaj   +1 more source

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate‐binding pocket

FEBS Letters, EarlyView.
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley   +1 more source

The Fission Yeast Cell Integrity Pathway: A Functional Hub for Cell Survival upon Stress and Beyond

Journal of Fungi, 2021
The survival of eukaryotic organisms during environmental changes is largely dependent on the adaptive responses elicited by signal transduction cascades, including those regulated by the Mitogen-Activated Protein Kinase (MAPK) pathways.
José Cansado, Teresa Soto, Alejandro Franco, Jero Vicente-Soler, Marisa Madrid   +4 more
doaj   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Glutathione and astaxanthin: Both enhance antioxidant capacity and regulate lipid metabolism in Pacific white shrimp (Litopenaeus vannamei) fed with high-lipid diets

Food Chemistry: Molecular Sciences
Glutathione (GSH) and astaxanthin (AX) have the function of helping lipid metabolism and antioxidant capacity; which one is better? The feeding experiment of an 8 weeks was conducted to investigate the advantages of these two working on Litopenaeus ...
Jiandong Chen, Tao Cheng, Xiang Si, Fan Yang, Jianhua Yi, Zhilong Yang, Zhaowen Li, Beiping Tan, Chunfeng Yao, Shuyan Chi   +9 more
doaj   +1 more source

The protein kinase Cmk2 negatively regulates the calcium/calcineurin signalling pathway and expression of calcium pump genes PMR1 and PMC1 in budding yeast

Cell Communication and Signaling, 2019
Through a genome-wide screen we have identified calcium-tolerant deletion mutants for five genes in the budding yeast Saccharomyces cerevisiae. In addition to CNB1 and RCN1 that are known to play a role in the calcium signalling pathway, the protein ...
Huihui Xu, Tianshu Fang, Hongbo Yan, Linghuo Jiang   +3 more
doaj   +1 more source

Streamlined and sensitive mono- and di-ribosome profiling in yeast and human cells [PDF]

, 2023
Lucas Ferguson, Heather E. Upton, Sydney C. Pimentel, Amanda Mok, Liana F. Lareau, Kathleen L. Collins, Nicholas T. Ingolia   +6 more
openalex   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

PICALM::MLLT10 translocated leukemia

FEBS Letters, EarlyView.
This comprehensive review of PICALM::MLLT10 translocated acute leukemia provides an in‐depth review of the structure and function of CALM, AF10, and the fusion oncoprotein (1). The multifaceted molecular mechanisms of oncogenesis, including nucleocytoplasmic shuttling (2), epigenetic modifications (3), and disruption of endocytosis (4), are then ...
John M. Cullen, Antonia C. Nakatsugawa, Natalie Barton, Henry Haines, Gary S. Stein, Janet L. Stein, Daniel S. Wechsler, Jessica L. Heath   +7 more
wiley   +1 more source