Results 151 to 160 of about 258,986 (286)

Correlating single-molecule rupture mechanics with cell population adhesion by yeast display. [PDF]

Biophys Rep (N Y), 2022
Santos MS, Liu H, Schittny V, Vanella R, Nash MA.   +4 more
europepmc   +1 more source

Diversity of Pharmaceuticals Enhances Antibiotic Resistance in the Invertebrate Gut via Biofilm‐Mediated Mechanisms

Advanced Science, EarlyView.
Pharmaceutical diversity acts as an independent driver of antibiotic resistance in soil invertebrates. While bulk soil remains unaffected, the collembolan gut microbiome exhibits significant resistance gene enrichment under complex chemical exposure and diurnal warming.
Yi‐Fei Wang, Ya‐Ning Wang, Da Lin, Jia‐Yang Xu, Feng‐Yuan Qi, Hui‐Ling Cui, Hui‐Jie Lu, Min Qiao, Edward Topp, Dong Zhu, Matthias C. Rillig, Yong‐Guan Zhu   +11 more
wiley   +1 more source

Proteome-Scale Screening to Identify High-Expression Signal Peptides with Minimal N-Terminus Biases via Yeast Display. [PDF]

ACS Synth Biol, 2022
Holec PV, Camacho KV, Breuckman KC, Mou J, Birnbaum ME.   +4 more
europepmc   +1 more source

Humanized and Charge‐Optimized CSPG4‐Specific CAR‐T Cells show Enhanced Efficacy against Head and Neck Squamous Cell Carcinoma

Advanced Science, EarlyView.
CSPG4 is identified as a high‐value, stemness‐associated target in HPV‐negative HNSCC. By implementing rational biophysical engineering, a humanized and charge‐optimized CAR is developed to overcome tonic signaling‐induced exhaustion. This strategy induces a profound transcriptomic shift toward a rejuvenated, stem‐like memory state, significantly ...
Xiang Xu, Shizhen Qiu, Zhitong Wang, Dan Li, Min Chen, Yinying Chu, Guangfei Li, Yi Fang, Changjiang Li, Fang Shi, Peijie He, Haitao Wu, Haopeng Wang, Jian Chen   +13 more
wiley   +1 more source

Measuring the capacity of yeast for surface display of cell wall‐anchored protein isoforms by using β‐lactamase as a reporter enzyme

FEBS Open Bio
Yeast surface display is a promising biotechnological tool that uses genetically modified yeast cell wall proteins as anchors for enzymes of interest, thereby transforming yeast cell wall into a living catalytic material. Here, we present a comprehensive
Tea Martinić Cezar, Antonia Paić, Stefani Prekpalaj, Renata Teparić, Bojan Žunar, Igor Stuparević   +5 more
doaj   +1 more source

A Protein-Engineered, Enhanced Yeast Display Platform for Rapid Evolution of Challenging Targets. [PDF]

ACS Synth Biol, 2021
Zahradník J, Dey D, Marciano S, Kolářová L, Charendoff CI, Subtil A, Schreiber G.   +6 more
europepmc   +1 more source

A Keratinocyte‐Mast Cell NF‐κB2/CXCL2/IL‐6 Amplification Loop Enhances Cutaneous Antifungal Defense Against C. albicans

Advanced Science, EarlyView.
ABSTRACT Mast cells (MCs), key innate immune sentinels at the host–environment interface, serve as primary responders to invading pathogens. However, their specific contribution to host defense against cutaneous Candida albicans (C. albicans) infection and their synergy with other immune and non‐immune cells remain poorly understood. Here, we show that
Yan Yuan, Manyun Mao, Jiaan Zhang, Zhimin Duan, JiaNing Wang, Yujie Chen, Sihan Chen, Chuting Liang, Xvyue Zhou, Songmei Geng, Min Li, Xu Chen, Ni Lian   +12 more
wiley   +1 more source

Selection and Characterization of Antibodies Recognizing Unnatural Base Pairs

Biologics
Background: Introducing unnatural base pairs into a natural, double-stranded DNA construct is a powerful tool within synthetic biology. Accordingly, the ability to detect these unnatural base pairs has many applications, including the study and detection
Antonietta M. Lillo, Nileena Velappan, Ruilian Wu, Madeline R. Bolding   +3 more
doaj   +1 more source

Reduction of therapeutic antibody self-association using yeast-display selections and machine learning. [PDF]

MAbs, 2022
Makowski EK, Chen H, Lambert M, Bennett EM, Eschmann NS, Zhang Y, Zupancic JM, Desai AA, Smith MD, Lou W, Fernando A, Tully T, Gallo CJ, Lin L, Tessier PM.   +14 more
europepmc   +1 more source

ZSL Orchestrates Synaptonemal Complex Assembly as a Central Region Scaffold to Ensure Synapsis Fidelity and Crossover Control in Polyploid Meiosis

Advanced Science, EarlyView.
A central molecular adaptor, ZSL, is identified that links transverse filaments ZYP1 to central element SCEP1/2 to drive synaptonemal complex assembly in Brassica napus. Loss of ZSL abolishes synaptonemal complex formation, disrupts meiotic chromosome segregation, and markedly increases crossovers, providing mechanistic insight into meiotic fidelity ...
Miaowei Geng, Shaochen Jia, Fei Cao, Cuiping Liu, Heshuang Zhang, Gang Xu, Jixin Zhuang, Yashi Zhang, Can Liu, Yuhang Zhao, Zilin Guo, Xinjie Yuan, Jiaqing Yang, Lei Chu, Bowei Cai, Hu Zhao, Chao Yang   +16 more
wiley   +1 more source