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Protein selection using yeast surface display
Methods, 2013Binding proteins are typically isolated from combinatorial libraries of scaffold proteins using one of the many library screening tools available, such as phage display, yeast surface display or mRNA display. A key principle underlying these screening technologies is the establishment of a link between each unique mutant protein and its corresponding ...
Nimish, Gera +2 more
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Differential display analysis of gene expression in yeast
Cellular and Molecular Life Sciences (CMLS), 2002RNA differential display (DD) is a powerful and straightforward method that employs random reverse-transcription polymerase chain reaction amplification of mRNA species with electrophoresis for comparative analysis of two or more transcriptomes. The small yeast genome represents a convenient model for studying basic functions of the eukaryotic genome ...
A V, Ivanova, S V, Ivanov
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Protein Engineering by Yeast Surface Display
Journal of Visualized ExperimentsProtein engineering enables the improvement of existing functions of a given protein or the generation of novel functions. One of the most widely used and versatile tools in the protein engineering field is yeast surface display, where a pool of randomized proteins is expressed on the surface of yeast.
Charlotte U, Zajc +3 more
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The Use of Yeast Surface Display in Biofuel Cells
2015Biofuel cells are electrochemical devices which convert chemical energy to electricity using biochemical pathways and redox enzymes. In enzymatic fuel cells purified redox enzymes catalyze the reactions in the anode and cathode compartments whereas in microbial fuel cells (MFCs) the entire metabolism of the microorganisms is exploited.
Alon Szczupak, Lital Alfonta
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Selection of Antibody Fragments by Yeast Display
2012The critical need for renewable, high-quality affinity reagents in biological research, as well as for diagnostic and therapeutic applications, has required the development of new platforms of discovery. Yeast display is one of the main methods of in vitro display technology with phage display. Yeast display has been chosen by numerous groups to refine
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Engineering Antibody Affinity by Yeast Surface Display
2004Publisher Summary This chapter elaborates the engineering antibody affinity by yeast surface display (YSD). YSD is a powerful tool for engineering the affinity, specificity, and stability of antibodies, as well as other proteins. The methods for displaying an antibody on yeast, creating mutant libraries and sorting libraries for improved clones are ...
David W, Colby +5 more
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Humanization of Chicken-Derived Antibodies by Yeast Surface Display
2022Chicken-derived antibodies emerged as a promising tool for diagnostic and therapeutic usage. Due to the phylogenetic distance between birds and mammals, chicken immunization campaigns with human antigens result in a chicken antibody (IgY) repertoire targeting epitopes not addressed by rodent-derived antibodies.
Jan P, Bogen +4 more
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Enhancement of display efficiency in yeast display system by vector engineering and gene disruption
Applied Microbiology and Biotechnology, 2009Vector engineering and gene disruption in host cells were attempted for the enhancement of alpha-agglutinin-based display of proteins on the cell surface in yeast. To evaluate the display efficiency by flow cytometric analysis, DsRed-monomer fused with FLAG-tag was displayed and immunostained as a model protein.
Kouichi, Kuroda +6 more
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Optimized protocol for antibody yeast display
2023Yeast display is an established technology used to discover proteins that have increased binding affinities to their targets. There are many steps within the process that affect the final binding affinity between proteins. The Maynard Lab regularly utilizes display technologies to discover antibodies that bind strongly to targets of interest prior to ...
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Yeast Display Guided Selection of pH-Dependent Binders
2022pH-dependent antigen binding has proven useful in engineering next-generation therapeutics specifically via antibody recycling technology. This technology allows for half-life extension, thereby lowering the amount and frequency of dosing of therapeutics. Cell sorting, coupled with display techniques, has been used extensively for the selection of high-
Jenna N, Meanor +3 more
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