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Ligand Engineering Using Yeast Surface Display
2014The genotype-phenotype linkage provided by display technologies enables efficient synthesis, analysis, and selection of combinatorial protein libraries. This approach tremendously expands the protein sequence space that can be efficiently evaluated for a selectable function.
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Protein Engineering and Selection Using Yeast Surface Display
2015Yeast surface display is a powerful technology for engineering a broad range of protein scaffolds. This protocol describes the process for de novo isolation of protein binders from large combinatorial libraries displayed on yeast by using magnetic bead separation followed by flow cytometry-based selection.
ANGELINI, Alessandro +8 more
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Analysis of Selective Gene Activation in Yeast by Differential Display
Methods, 1998TATA box binding protein-associated factors (TAF(II)s) are dispensable for transcription of most genes in yeast. To further investigate the in vivo functions of TAF(II)s, differential display was used to identify a small subset of yeast genes whose transcription is dependent on yeast TAF(II)145, the core TAF(II) component that contacts TATA box-binding
W C, Shen, M R, Green
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Simultaneous Yeast Display for Medical Biosensors
2022Biosensors play an important role in medical diagnostics, industrial biotechnology, and environmental monitoring. A key challenge in biosensor design is developing complementary ligand binding domains which bind to the small molecule in a sandwich like fashion, causing fused biosensor output domains to co-localise and transduce a signal.
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Yeast surface display for screening combinatorial polypeptide libraries
Nature Biotechnology, 1997Display on the yeast cell wall is well suited for engineering mammalian cell-surface and secreted proteins (e.g., antibodies, receptors, cytokines) that require endoplasmic reticulum-specific post-translational processing for efficient folding and activity.
E T, Boder, K D, Wittrup
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Identification of Yeast Meiosis-Specific Genes by Differential Display
Methods, 1998Meiosis, a specialized cell division process, occurs in all sexually reproducing organisms. During this process a diploid cell undergoes a single round of DNA replication followed by two rounds of nuclear division to produce four haploid gametes. In yeast, the meiotic products are packaged into four spores that are enclosed in a sac known as an ascus ...
D K, Nag, J, Axelrod
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A facile yeast-display approach for antibody mask discovery
Protein Engineering, Design and SelectionAbstract Tuning in vivo activity of protein therapeutics can improve their safety. In this vein, it is possible to add a ‘mask’ moiety to a protein therapeutic such that its ability to bind its target is prevented until the mask has been proteolytically removed, for instance by a tumor-associated protease. As such, new methods to isolate
Nithya M Badarinath +13 more
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Yeast surface display technology: Mechanisms, applications, and perspectives
Biotechnology AdvancesMicrobial cell surface display technology, which relies on genetically fusing heterologous target proteins to the cell wall through fusion with cell wall anchor proteins, has emerged as a promising and powerful method with diverse applications in biotechnology and biomedicine.
Yibo, Li +3 more
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Surface display of proteins in yeasts
2014Surface display of proteins in bacteria and yeasts has proved to be an interesting method of protein immobilization for different purposes. Generally, proteins of interest are genetically engineered by fusing them to autochthonous cell wall proteins, or parts of autochthonous proteins directing them to the wall and anchoring them at the cell surface ...
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