Results 241 to 250 of about 258,986 (286)

Engineering antibodies by yeast display

Archives of Biochemistry and Biophysics, 2012
Since its first application to antibody engineering 15 years ago, yeast display technology has been developed into a highly potent tool for both affinity maturing lead molecules and isolating novel antibodies and antibody-like species. Robust approaches to the creation of diversity, construction of yeast libraries, and library screening or selection ...
Eric T, Boder, Maryam, Raeeszadeh-Sarmazdeh, J Vincent, Price   +2 more
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Yeast cell-surface display?applications of molecular display

Applied Microbiology and Biotechnology, 2004
In a cell-surface engineering system established using the yeast Saccharomyces cerevisiae, novel, so-called arming yeasts are constructed that are armed with biocatalysts in the form of enzymes, functional proteins, antibodies, and combinatorial protein libraries.
A, Kondo, M, Ueda
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Yeast surface display for screening combinatorial polypeptide libraries

Nature Biotechnology, 1997
Display on the yeast cell wall is well suited for engineering mammalian cell-surface and secreted proteins (e.g., antibodies, receptors, cytokines) that require endoplasmic reticulum-specific post-translational processing for efficient folding and activity.
E T, Boder, K D, Wittrup
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Yeast surface display technology: Mechanisms, applications, and perspectives

Biotechnology Advances
Microbial cell surface display technology, which relies on genetically fusing heterologous target proteins to the cell wall through fusion with cell wall anchor proteins, has emerged as a promising and powerful method with diverse applications in biotechnology and biomedicine.
Yibo, Li, Xu, Wang, Ning-Yi, Zhou, Junmei, Ding   +3 more
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Protein selection using yeast surface display

Methods, 2013
Binding proteins are typically isolated from combinatorial libraries of scaffold proteins using one of the many library screening tools available, such as phage display, yeast surface display or mRNA display. A key principle underlying these screening technologies is the establishment of a link between each unique mutant protein and its corresponding ...
Nimish, Gera, Mahmud, Hussain, Balaji M, Rao   +2 more
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Quantification analysis of yeast-displayed lipase

Analytical Biochemistry, 2014
We describe a method for quantification of displayed lipase on yeast cell surface. The strategy uses an organophosphonate ester to irreversibly inhibit the active lipase and release a detectable fluorescent group. The amount of displayed lipase can be represented as "g/g cell" or "molecules/cell".
Zi, Jin, Kun, Zhang, Li, Zhang, Sui-Ping, Zheng, Shuang-Yan, Han, Ying, Lin   +5 more
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Protein Engineering by Yeast Surface Display

Journal of Visualized Experiments
Protein engineering enables the improvement of existing functions of a given protein or the generation of novel functions. One of the most widely used and versatile tools in the protein engineering field is yeast surface display, where a pool of randomized proteins is expressed on the surface of yeast.
Charlotte U, Zajc, Elise, Sylvander, Magdalena, Teufl, Michael W, Traxlmayr   +3 more
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Optimized protocol for antibody yeast display

2023
Yeast display is an established technology used to discover proteins that have increased binding affinities to their targets. There are many steps within the process that affect the final binding affinity between proteins. The Maynard Lab regularly utilizes display technologies to discover antibodies that bind strongly to targets of interest prior to ...
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