An unfolded protein-induced conformational switch activates mammalian IRE1. [PDF]
, 2017 The unfolded protein response (UPR) adjusts the cell's protein folding capacity in the endoplasmic reticulum (ER) according to need. IRE1 is the most conserved UPR sensor in eukaryotic cells.
Acosta-Alvear, Diego +5 more
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Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time [PDF]
, 2015 We present a single-molecule tool called the CoPro (Concentration of Proteins) method that uses millisecond imaging with convolution analysis, automated image segmentation and super-resolution localization microscopy to generate robust estimates for ...
Leake, Mark C., Wollman, Adam J. M.
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AntibodiesThe optimization of the affinity of monoclonal antibodies is crucial for the development of drug candidates, as it can impact the efficacy of the drug and, thus, the dose and dosing regimen, limit adverse effects, and reduce therapy costs.
Julia Harwardt +5 more
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Specific Targeting of Lymphoma Cells Using Semisynthetic Anti-Idiotype Shark Antibodies
Frontiers in Immunology, 2020 The B-cell receptor (BCR) is a key player of the adaptive immune system. It is a unique part of immunoglobulin (Ig) molecules expressed on the surface of B cells. In case of many B-cell lymphomas, the tumor cells express a tumor-specific and functionally
Arturo Macarrón Palacios +8 more
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EXPLoRA-web: linkage analysis of quantitative trait loci using bulk segregant analysis [PDF]
, 2016 Identification of genomic regions associated with a phenotype of interest is a fundamental step toward solving questions in biology and improving industrial research.
Duitama, Jorge +2 more
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Engineered ipilimumab variants that bind human and mouse CTLA-4
mAbsTesting of candidate monoclonal antibody therapeutics in preclinical models is an essential step in drug development. Identification of antibody therapeutic candidates that bind their human targets and cross-react to mouse orthologs is often challenging,
Brett Robison +12 more
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Intracellular Regulatory Networks are close to Monotone Systems [PDF]
, 2007 Several meso-scale biological intracellular regulatory networks that have specified directionality of interactions have been recently assembled from experimental literature.
Avi Ma' +2 more
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AlphaBind, a domain-specific model to predict and optimize antibody–antigen binding affinity
mAbsAntibodies are versatile therapeutic molecules that use combinatorial sequence diversity to cover a vast fitness landscape. Designing optimal antibody sequences, however, remains a major challenge.
Aditya A. Agarwal +14 more
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The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken [PDF]
, 2005 The replacement histone H2A.Z is variously reported as being linked to gene expression and preventing the spread of heterochromatin in yeast, or concentrated at heterochromatin in mammals.
Bonifer, C. +8 more
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Microbial Cell Factories, 2018 Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role in protein engineering among the high-throughput display methodologies that have been developed to date. The classical yeast display technology relies on tethering
Doreen Könning, Harald Kolmar
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