Results 101 to 110 of about 258,986 (286)

Amyloidogenic Peptide Fragments Designed From Bacterial Collagen‐like Proteins Form Hydrogel

Advanced Functional Materials, EarlyView.
This study identified amyloidogenic sequence motifs in bacterial collagen‐like proteins and exploited these to design peptides that self‐assemble into β‐sheet fibers and form hydrogels. One hydrogel supported healthy fibroblast growth, showing promise for biocompatible materials. Our work demonstrates that bacterial sequences can be harnessed to create
Vamika Sagar, Xuen J Ng, Lukas Lechel, Ashish Chand Rajwar, Hendrik Bargel, Abhishek A. Jalan   +5 more
wiley   +1 more source

Engineered ipilimumab variants that bind human and mouse CTLA-4

mAbs
Testing of candidate monoclonal antibody therapeutics in preclinical models is an essential step in drug development. Identification of antibody therapeutic candidates that bind their human targets and cross-react to mouse orthologs is often challenging,
Brett Robison, SJ Diong, Anusha Kumar, Thomas M. Moon, Olin Chang, Bryant Chau, Christine Bee, Ishita Barman, Arvind Rajpal, Alan J. Korman, Sean M. West, Pavel Strop, Peter S. Lee   +12 more
doaj   +1 more source

Dss1 is a 26S proteasome ubiquitin receptor [PDF]

, 2014
The ubiquitin-proteasome system is the major pathway for protein degradation in eukaryotic cells. Proteins to be degraded are conjugated to ubiquitin chains that act as recognition signals for the 26S proteasome.
Arrigoni, Bohn, Choi, Cornilescu, Deveraux, Dosztányi, Ellisdon, Faza, Finley, Funakoshi, Groll, Hartmann-Petersen, Husnjak, Husnjak, Jossé, Kjaergaard, Kriegenburg, Matsuyama, Mayor, Moreno, Obradovic, Peth, Saeki, Sakata, Schreiner, Seeger, Sone, Spratt, Su, Tomko, Uversky, Verma, Verma, Vogt, Wilkinson, Yang   +35 more
core   +5 more sources

Nanodiamond Quantum Sensors for Probing Free Radical Biology

Advanced Functional Materials, EarlyView.
Free radicals play key roles in cellular signaling and disease but remain difficult to measure in living systems. Nanodiamonds (NDs) with nitrogen‐vacancy (NV) centers enable quantum sensing of local magnetic noise via T₁ relaxometry, providing nondestructive radical detection in living cells.
Qi Lu, Yingke Wu, Tanja Weil
wiley   +1 more source

AlphaBind, a domain-specific model to predict and optimize antibody–antigen binding affinity

mAbs
Antibodies are versatile therapeutic molecules that use combinatorial sequence diversity to cover a vast fitness landscape. Designing optimal antibody sequences, however, remains a major challenge.
Aditya A. Agarwal, James Harrang, David Noble, Kerry L. McGowan, Adrian W. Lange, Emily Engelhart, Miranda C. Lahman, Jeffrey Adamo, Xin Yu, Oliver Serang, Kyle J. Minch, Kimberly Y. Wellman, David A. Younger, Randolph M. Lopez, Ryan O. Emerson   +14 more
doaj   +1 more source

Coating Artificial Spider Silk Fiber with Magnetic FeCo: An Effective Strategy for Creating a Flexible Magneto‐Responsive Material

Advanced Functional Materials, EarlyView.
An original method is presented for producing artificial spider silk fibers with magnetic and magnetomechanical responsiveness, which consists in coating them with a nanometer‐thick layer of FeCo alloy by sputtering deposition. The challenge of combining organic materials and inorganic magnetic nanostructures is addressed, thus taking a step forward ...
Filippo Lanaro, Federico Spizzo, Gabriele Greco, Benjamin Schmuck, Anna Rising, Lucia Del Bianco   +5 more
wiley   +1 more source

Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display

Microbial Cell Factories, 2018
Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role in protein engineering among the high-throughput display methodologies that have been developed to date. The classical yeast display technology relies on tethering
Doreen Könning, Harald Kolmar
doaj   +1 more source

Intracellular Regulatory Networks are close to Monotone Systems [PDF]

, 2007
Several meso-scale biological intracellular regulatory networks that have specified directionality of interactions have been recently assembled from experimental literature.
Avi Ma', Eduardo D. Sontag, Ravi Iyengar   +2 more
core   +1 more source

Lysosome‐Acidifying Nanoparticles Rescue A30P α‐Synuclein Induced Neuronal Death in Cellular and Drosophila Models of Parkinson's Disease

Advanced Healthcare Materials, EarlyView.
Lysosome‐targeted acidic nanoparticles based on a biodegradable poly(ethylene tetrafluorosuccinate‐co‐succinate) copolymer are engineered to restore impaired lysosomal acidification through pH‐responsive intracellular degradation. Localized acid release enhances autophagic proteolysis, reduces α‐synuclein accumulation, and preserves dopaminergic neuron
Chih Hung Lo, Mengda Ren, Gavin Wen Zhao Loi, Eka Norfaishanty Saipuljumri, Jonathan Indajang, Kah Leong Lim, Jialiu Zeng   +6 more
wiley   +1 more source

The cell surface receptor Tartan is a potential in vivo substrate for the receptor tyrosine phosphatase Ptp52F [PDF]

, 2009
Receptor-linked protein-tyrosine phosphatases (RPTPs) are essential regulators of axon guidance and synaptogenesis in Drosophila, but the signaling pathways in which they function are poorly defined.
Bugga, Lakshmi, Ratnaparkhi, Anuradha, Zinn, Kai   +2 more
core   +2 more sources