Results 101 to 110 of about 3,034 (205)

Scale‐Down Platforms for Process‐Oriented Enzyme Screening Using Microfluidics

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Enzyme screening platforms are typically evaluated based on their throughput and analytical performance rather than their ability to mimic the conditions encountered at large scale. In bioreactors, enzymes experience gradients in substrate concentration, oxygen availability, pH, temperature, and interfacial exposure, caused by imperfect mixing
Maria I. Rodriguez‐Torres, Elif Erdem, Junhyeong Wang, Ulrich Krühne, Michael Daniele, John M. Woodley   +5 more
wiley   +1 more source

Cryo‐EM Analysis of Adenovirus Vector Reveals Purification Conditions Leading to Loss of Viral Fiber Proteins and Reduced Infectivity

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Vaccines and gene therapies require efficient delivery of genetic material to host cells. Viral vectors leverage viruses' ability to enter host cells to achieve this. Following the SARS‐CoV‐2 vaccine and novel gene therapies that utilize adenovirus vectors, there has been an increase in the demand for large‐scale production of vectors.
Betsy Widdicombe, Joseph D. Barritt, Nichakorn Pipatpadungsin, Braulio Carrillo Sanchez, Dale Stibbs, Lisa Cooper, Sarah L. Rouse, Daniel G. Bracewell   +7 more
wiley   +1 more source

Tackling cancer stemness with nanotechnology in the era of precision medicine

BMEMat, EarlyView.
Precise customization of nanoparticles (NPs) enables active targeting of cancer stem cells (CSCs), thereby improving drug delivery and therapeutic efficacy. NP‐based probing enhances CSC detection through imaging and liquid biopsy, whereas diverse therapeutic payloads improve therapeutic outcomes.
Shaolei Guo, Shun Yao, Lihui Meng, Chunyuan Chen, Yuechu Lucinda Lu, Rong Lu, Kashif Rafiq Zahid, Umar Raza, Qingzhi Wu, Wenli Chen   +9 more
wiley   +1 more source

β-galactosidases from cowpea stems: properties and gene expression under conditions of salt stress

Revista Ciência Agronômica, 2014
The aim of this work was to evaluate the properties and gene expression of isoforms of β-galactosidase, isolated from the stems of seedlings of the cowpea cv. Pitiuba, at different stages of development and sown in the absence and presence of NaCl. Seeds
Fabrício Bonfim Sudério, Enéas Gomes Filho, José Hélio Costa, Joaquim Enéas Filho   +3 more
doaj  

Immobilization of β-Galactosidases on the <i>Lactobacillus</i> Cell Surface Using the Peptidoglycan-Binding Motif LysM. [PDF]

Catalysts, 2019
Pham ML, Tran AM, Kittibunchakul S, Nguyen TT, Mathiesen G, Nguyen TH.   +5 more
europepmc   +1 more source

Increased Insulin Action, Glucose Metabolism and Muscle Function in Supervillin‐Knockout and Supervillin‐Mutant Mice

Cytoskeleton, EarlyView.
ABSTRACT We here describe mouse models with complementary homozygous Svil mutations. In skeletal muscle, Svil‐Mut mice express the Svil‐encoded N‐terminus fused to the βgal‐neo gene‐trap tag and lack the highly conserved archvillin C‐terminus; Svil‐KO mice lack expression of all known Svil‐encoded proteins; and Svil‐LoxP mice contain loxP sites for ...
Tara C. Smith, Lauren A. Tauer, Randall H. Friedline, Xiaodi Hu, Duy Tran, Yangyi E. Luo, Nacima Hadjout‐Rabi, Elisabeth R. Barton, Jason K. Kim, Elizabeth J. Luna   +9 more
wiley   +1 more source

Bacterial Species Associated With Chronic Inflammation and Oral Cancer. A Comparative Study Between Patients With HNC and Patients With Chronic Periodontitis

Cancer Nexus, EarlyView.
ABSTRACT Objective To compare the presence and quantification of selected periodontopathogenic bacterial species and salivary parameters between patients with head and neck cancer (HNC) treated with radiotherapy and patients with chronic periodontitis, using chronic periodontitis as a reference model of oral dysbiosis.
Tanya Pereira‐Riveros, José López‐López, Alicia Lozano‐Borbalas, Julio Paciello‐Coronel, Teresa Vinuesa   +4 more
wiley   +1 more source

Fluorinated Iminosugar Glycomimetics: Synthesis and Activity as GCase Ligands

European Journal of Organic Chemistry, EarlyView.
DAST‐mediated deoxyfluorination of suitably functionalized piperidines provided regio‐ and stereoselective access to fluorinated piperidine and pyrrolidine glycomimetics, the latter arising from an unusual ring‐contraction process. Fluorination generally enhanced β‐glucocerebrosidase (GCase) inhibitory potency, but lost PC activity toward L444P/L444P ...
Francesca Mangiavacchi, Alessio Morano, Cosimo Margheri, Cristina Faggi, Amelia Morrone, Camilla Matassini, Francesca Clemente, Andrea Goti, Martina Cacciarini, Francesca Cardona   +9 more
wiley   +1 more source

Recombinant Expression and Functional Analysis of GH2 β-Galactosidases Derived from <i>Lactobacillus delbrueckii</i> subsp. <i>bulgaricus</i> NSP1 and <i>Streptococcus thermophilus</i> RB16. [PDF]

Int J Mol Sci
Shamsiyeva Y, Kostanova A, Tursunbekova A, Khassenov B, Akishev Z.   +4 more
europepmc   +1 more source

From Enrichment to Insight: Leveraging Preparative cIEF Enrichments and Characterization to Support Data‐Driven Integration Practices for cIEF‐based Analytics

ELECTROPHORESIS, EarlyView.
Preparative cIEF enrichment enabled isolation of a low‐abundance, poorly resolved basic charge variant identified during routine cIEF analysis. Orthogonal characterization by peptide mapping and SCX‐UV–MS revealed incomplete N‐terminal pyroglutamate formation and Fc methionine oxidation.
Ramya Rao, Dominik Herrmann, Seamus Kelley, Victoria Cotham, Logan Figular, Youmi Moon, Deanna Di Grandi, Kathir Muthusamy, Yue Fu, Nisha Palackal, Shunhai Wang, Ning Li, Jennifer B. Nguyen, Michael P. Rosconi, Erica A. Pyles   +14 more
wiley   +1 more source