Results 101 to 110 of about 4,834 (282)
Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum [PDF]
, 2013 2,5-diketo-D-gluconic acid reductase (2,5-DKG reductase) catalyses the reduction of 2,5-diketo-D-gluconic acid (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG), a direct precursor (lactone) of L-ascorbic acid (vitamin C).
Herbert Michlmayr +4 more
core +1 more source
Advanced Science, EarlyView.Antibody–drug conjugates (ADCs) transform breast cancer therapy, yet resistance limits their durability. Emerging evidence reveals that ADC failure is not solely tumor‐intrinsic but shaped by dynamic tumor–microenvironment interactions that alter drug delivery, processing, and response.
Minji Seo, Jangsoon Lee, Naoto T. Ueno
wiley +1 more source
Bioactive phenolic compounds : production and extraction by solid-state fermentation. A review [PDF]
, 2011 Interest in the development of bioprocesses for the production or extraction of bioactive compounds from natural sources has increased in recent years due to the potential applications of these compounds in food, chemical, and pharmaceutical industries ...
Aguilar Gonzalez, Cristobal Noé +5 more
core +1 more source
Advanced Science, EarlyView.TB, a conventional cell viability dye, acts as both a fluorescence modulator (quenching GFP fluorescence upon protein binding and recovering it upon release) and a photosensitizer that triggers endosomal escape via photochemical internalization (PCI) under 590 nm light.
Zhongqi Yao +4 more
wiley +1 more source
Temporal expression dynamics of plant biomass-degrading enzymes by a synthetic bacterial consortium growing on sugarcane bagasse [PDF]
, 2018 Plant biomass (PB) is an important source of sugars useful for biofuel production, whose degradation efficiency depends on synergistic and dynamic interactions of different enzymes.
Chaib De Mares, Maryam +2 more
core +1 more source
Low copy number vectors for expression of fused genes to β-galactosidase inEscherichia coli [PDF]
FEMS Microbiology Letters, 1991 A series of six expression vectors, pXM184Lac.A, B, C, pXM184Z.A, B, C, based on the low copy plasmid pACYC184 that allow for expression of proteins fused to beta-galactosidase in Escherichia coli is described. A level of 50,000 units of beta-galactosidase is routinely observed and is easily identifiable on protein gels.
openaire +2 more sources
Advanced Science, EarlyView.The butterfly unfolded wing in an open form structure of botulinum neurotoxin type A (BoNT/A) at physiological‐state was confirmed at 2.85 Å resolution by cryo‐electron microscopy (cryo‐EM). Structure‐guided protein engineering significantly enhanced the receptor‐binding affinity, therapeutic efficacy, and safety of the engineered toxin variants ...
Wenrui Wang +16 more
wiley +1 more source
Lactobacillus delbrueckii ssp. lactis and ssp. bulgaricus: a chronicle of evolution in action [PDF]
, 2014 Lactobacillus delbrueckii ssp. lactis and ssp. bulgaricus are lactic acid producing bacteria that are largely used in dairy industries, notably in cheese-making and yogurt production.
Benoît Quinquis +12 more
core +4 more sources
Purification and some properties of phospho-β-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201 [PDF]
FEMS Microbiology Letters, 2002 Phospho-beta-galactosidase (P-beta-gal; EC 3.2.1.85) is induced during growth of Leptotrichia buccalis ATCC 14201 on lactose and lactulose. The enzyme has been purified to electrophoretic homogeneity (M(r) approximately 53 kDa, pI approximately 4.8), and kinetic parameters have been determined using the chromogenic analog o-nitrophenyl-beta-D ...
openaire +2 more sources
Self‐Assembling Hybrid Hydrogel Reprograms the Stromal Vascular Fraction to Treat Osteoarthritis
Advanced Science, EarlyView.This study presents a bioinspired injectable hydrogel that enhances the therapeutic potential of stem cell‐rich stromal vascular fraction for treating osteoarthritis. By reprogramming cell behavior through epigenetic modulation, the hydrogel promotes cartilage regeneration and reduces joint damage in a rat model, offering a promising new approach for ...
Waifang Hou +23 more
wiley +1 more source