Results 31 to 40 of about 937,622 (274)

Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a. [PDF]

, 2014
Surfactant-mediated removal of proteins from biomembranes invariably results in partial or complete loss of function and disassembly of multi-protein complexes. We determined the capacity of styrene-co-maleic acid (SMA) co-polymer to remove components of
Taylor, Peter W, Paulin, S, Roper, David I, Garcia-Lara, J, Roper, DI, Taylor, PW, Galley, Nicola F., Paulin, Sarah, Rosado, H, Foster, SJ, Taylor, Peter W., Dafforn, TR, Dafforn, Tim, Dafforn, Timothy R, Galley, Nicola F, Jamshad, Mohammed, Rosado, Helena, Foster, Simon J, Garcia-Lara, Jorge, Jamshad, M, Galley, NF, Foster, Simon J., Roper, David I.   +22 more
core   +1 more source

Contributions of β-lactamase substrate specificity and outer membrane permeability to the antibiotic sheltering of β-lactam-susceptible bacteria [PDF]

RSC Chemical Biology
The use of β-lactam antibiotics is threatened by antibiotic resistant bacteria that produce β-lactamases. These enzymes not only protect the bacteria that produce them but also shelter other bacteria in the same environment that would otherwise be ...
Montserrat Mora-Ochomogo, Mitchell A. Jeffs, Josephine L. Liu, Christopher T. Lohans   +3 more
doaj   +1 more source

Detection of an IS21 insertion sequence in the mexR gene of Pseudomonas aeruginosa increasing β-lactam resistance [PDF]

FEMS Microbiology Letters, 2004
To understand the regulation of the MexAB OprM efflux system in a clinical strain of Pseudomonas aeruginosa presenting a decreased susceptibility to ticarcillin and aztreonam, the mexR repressor gene was amplified by polymerase chain reaction (PCR) and was shown to be disrupted by an insertion sequence of more than 2 kb, with characteristic direct and ...
Boutoille, David, Corvec, Stéphane, Caroff, Nathalie, Giraudeau, Cécile, Espaze, Eric, Caillon, Jocelyne, Plesiat, Patrick, Reynaud, Alain   +7 more
openaire   +2 more sources

Thefiblocus inStreptococcus pneumoniaeis required for peptidoglycan crosslinking and PBP-mediated β-lactam resistance [PDF]

FEMS Microbiology Letters, 2000
Penicillin resistance in pneumococci is mediated by modified penicillin-binding proteins (PBPs) that have decreased affinity to beta-lactams. In high-level penicillin-resistant transformants of the laboratory strain Streptococcus pneumoniae R6 containing various combinations of low-affinity PBPs, disruption of the fib locus results in a collapse of PBP-
B, Weber, K, Ehlert, A, Diehl, P, Reichmann, H, Labischinski, R, Hakenbeck   +5 more
openaire   +2 more sources

Reversible inactivation of a peptidoglycan transpeptidase by a β-lactam antibiotic mediated by β-lactam-ring recyclization in the enzyme active site

Scientific Reports, 2017
β-lactam antibiotics act as suicide substrates of transpeptidases responsible for the last cross-linking step of peptidoglycan synthesis in the bacterial cell wall.
Zainab Edoo, Michel Arthur, Jean-Emmanuel Hugonnet   +2 more
doaj   +1 more source

Characterization of the membrane sensor PenJ for β-lactam antibodies fromBacillus licheniformisby amino acid substitution [PDF]

FEMS Microbiology Letters, 1993
The PenJ protein of the penicillinase gene (penP) expression system from Bacillus licheniformis is an antirepressor and membrane receptor for beta-lactam antibiotics. A putative beta-lactam antibiotic binding site including Ser402 and Lys405, which are homologous to the conserved sequence for the beta-lactam binding site (Ser-X-X-Lys) is present.
M, Takagi, T, Ohta, S, Johki, T, Imanaka
openaire   +2 more sources

An update on the synthesis and reactivity of spiro-fused β-lactams

Arkivoc, 2019
Beta-Lactam ring-containing compounds play a pivotal role in drug design and synthetic chemistry. Spirocyclic beta-lactams, representing an important beta-lactam subclass, have recently attracted considerable interest with respect to new synthetic methodologies and pharmacological applications.
Dao Thi, Hang, D'hooghe, Matthias
openaire   +2 more sources

Restoration of effectiveness of β-lactams on methicillin-resistantStaphylococcus aureusby tellimagrandin I from rose red [PDF]

FEMS Microbiology Letters, 2000
We found that extract from petals of Rosa canina L. (rose red) strikingly reduced the minimum inhibitory concentration of beta-lactams in methicillin-resistant Staphylococcus aureus. We isolated two compounds that reduced the minimum inhibitory concentrations of beta-lactams from the extract, tellimagrandin I and rugosin B.
S, Shiota, M, Shimizu, T, Mizusima, H, Ito, T, Hatano, T, Yoshida, T, Tsuchiya   +6 more
openaire   +2 more sources

Decreased membrane permeability in a polymyxin B-resistantEscherichia colimutant exhibiting multiple resistance to β-lactams as well as aminoglycosides [PDF]

FEMS Microbiology Letters, 1998
A laboratory mutant of Escherichia coli stably resistant to more than 36,000 U ml-1 of polymyxin B was isolated. The mutant exhibited moderate increases in minimum inhibitory concentration to fluoroquinolones and bacitracin but high levels of cross-resistance to beta-lactams and aminoglycosides.
S O, Rahaman, J, Mukherjee, A, Chakrabarti, S, Pal   +3 more
openaire   +2 more sources

Ethane-β-Sultam Modifies the Activation of the Innate Immune System Induced by Intermittent Ethanol Administration in Female Adolescent Rats [PDF]

, 2014
Intermittent ethanol abuse or ‘binge drinking’ during adolescence induces neuronal damage, which may be associated with cognitive dysfunction. To investigate the neurochemical processes involved, rats were administered either 1 g/kg or 2 g/kg ethanol in ...
Colivicchi Ma, Stefanini C, Hemmings, K., Colivicchi, M.A., Stefanini, C., Della Corte, L., Dexter, D T, De Witte, P, Colivicchi, Stefanini C, Nayak, K., de Witte, Philippe, Pitard, A., Page, M.I., Corte, Della, Pittard, A., Lallemand, f, Ward, Roberta, Ward, Roberta J., Hemmings, Karl, Hemming, Karl, Lallemand, Frederic, Page, Michael I., Dexter, D. T., Dexter, David T., Page, Michael, Nayak, K, Corte, Laura Della   +25 more
core   +1 more source