Results 21 to 30 of about 29,069 (201)

Limited Substrate Specificity of PS/γ-Secretase Is Supported by Novel Multiplexed FRET Analysis in Live Cells

Biosensors, 2021
Presenilin (PS)/γ-secretase is an aspartyl protease that processes a wide range of transmembrane proteins such as the amyloid precursor protein (APP) and Notch1, playing essential roles in normal biological events and diseases.
Mei C. Q. Houser, Yuliia Turchyna, Florian Perrin, Lori Chibnik, Oksana Berezovska, Masato Maesako   +5 more
doaj   +1 more source

Visualization of Alzheimer’s Disease Related α-/β-/γ-Secretase Ternary Complex by Bimolecular Fluorescence Complementation Based Fluorescence Resonance Energy Transfer

Frontiers in Molecular Neuroscience, 2018
The competitive ectodomain shedding of amyloid-β precursor protein (APP) by α-secretase and β-secretase, and the subsequent regulated intramembrane proteolysis by γ-secretase are the key processes in amyloid-β peptides (Aβ) generation.
Xin Wang, Gang Pei, Gang Pei
doaj   +1 more source

Targeting Amyloidogenic Processing of APP in Alzheimer’s Disease

Frontiers in Molecular Neuroscience, 2020
Alzheimer’s disease (AD) is the most common type of senile dementia, characterized by neurofibrillary tangle and amyloid plaque in brain pathology. Major efforts in AD drug were devoted to the interference with the production and accumulation of amyloid ...
Jing Zhao, Xinyue Liu, Weiming Xia, Weiming Xia, Yingkai Zhang, Chunyu Wang, Chunyu Wang, Chunyu Wang   +7 more
doaj   +1 more source

Caveolin‐1 inhibition mediates the opposing effects of alcohol on γ‐secretase activity in arterial endothelial and smooth muscle cells

Physiological Reports, 2023
Notch is important to vessel homeostasis. We investigated the mechanistic role of caveolin‐1 (Cav‐1) in mediating the effects of alcohol (Ethanol/EtOH) on the γ‐secretase proteolytic activity necessary for Notch signaling in vascular cells.
Naresh K. Rajendran, Weimin Liu, Paul A. Cahill, Eileen M. Redmond   +3 more
doaj   +1 more source

Molecular mechanism of the intramembrane cleavage of the β-carboxyl terminal fragment of amyloid precursor protein by γ-secretase [PDF]

Frontiers in Physiology, 2014
Amyloid β-protein (Aβ) plays a central role in the pathogenesis of Alzheimer's disease, the most common age-associated neurodegenerative disorder. Aβ is generated through intramembrane proteolysis of the β-carboxyl terminal fragment (βCTF) of β-amyloid precursor protein (APP) by γ-secretase.
openaire   +3 more sources

Determination of the Proteolytic Cleavage Sites of the Amyloid Precursor-Like Protein 2 by the Proteases ADAM10, BACE1 and γ-Secretase

PLoS ONE, 2011
Regulated intramembrane proteolysis of the amyloid precursor protein (APP) by the protease activities α-, β- and γ-secretase controls the generation of the neurotoxic amyloid β peptide. APLP2, the amyloid precursor-like protein 2, is a homolog of APP, which shows functional overlap with APP, but lacks an amyloid β domain.
Hogl, Sebastian, Kuhn, Peer-Hendrik, Colombo, Alessio, Lichtenthaler, Stefan F.   +3 more
openaire   +5 more sources

In vitro validation of γ-secretase inhibitors alone or in combination with other anti-cancer drugs for the treatment of T-cell acute lymphoblastic leukemia

Haematologica, 2008
Background Activating NOTCH1 mutations are common in T-cell acute lymphoblastic leukemia. Inhibition of NOTCH1 signaling with γ-secretase inhibitors causes cell cycle block, but only after treatment for several days.
Kim De Keersmaecker, Idoya Lahortiga, Nicole Mentens, Cedric Folens, Leander Van Neste, Sofie Bekaert, Peter Vandenberghe, Maria D. Odero, Peter Marynen, Jan Cools   +9 more
doaj   +1 more source

Cholesterol-Dependent γ-Secretase Activity in Buoyant Cholesterol-Rich Membrane Microdomains

Neurobiology of Disease, 2002
Buoyant membrane fractions containing presenilin 1 (PS1), an essential component of the γ-secretase complex, and APP CTFβ, a γ-secretase substrate, can be isolated from cultured cells and brain by several different fractionation procedures that are ...
Suzanne Wahrle, Pritam Das, Andrew C. Nyborg, Chris McLendon, Mikio Shoji, Takeshi Kawarabayashi, Linda H. Younkin, Steven G. Younkin, Todd E. Golde   +8 more
doaj   +1 more source

A Novel NIR-FRET Biosensor for Reporting PS/γ-Secretase Activity in Live Cells

Sensors, 2020
Presenilin (PS)/γ-secretase plays a pivotal role in essential cellular events via proteolytic processing of transmembrane proteins that include APP and Notch receptors. However, how PS/γ-secretase activity is spatiotemporally regulated by other molecular
Mei CQ Houser, Steven S Hou, Florian Perrin, Yuliia Turchyna, Brian J Bacskai, Oksana Berezovska, Masato Maesako   +6 more
doaj   +1 more source

Functional γ-secretase complex assembly in Golgi/trans-Golgi network: interactions among presenilin, nicastrin, Aph1, Pen-2, and γ-secretase substrates

Neurobiology of Disease, 2003
γ-Secretase is a proteolytic complex whose substrates include Notch, β-amyloid precursor protein (APP), and several other type I transmembrane proteins. Presenilin (PS) and nicastrin are known components of this high-molecular-weight complex, and recent ...
Stephanie Baulac, Matthew J LaVoie, W.Taylor Kimberly, Jennifer Strahle, Michael S Wolfe, Dennis J Selkoe, Weiming Xia   +6 more
doaj   +1 more source