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腺病毒介导血管内皮细胞生长因子基因体外转染骨髓间充质干细胞 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】探讨腺病毒介导血管内皮细胞生长因子(VEGF165)基因转染大鼠骨髓间充质干细胞(MSCs)后目的基因表达情况及对MSCs增殖分化的影响。【方法】构建含VEGF基因的腺病毒表达载体,利用贴壁法分离培养MSCs后,用荧光显微镜和流式细胞仪检测转染效果和转染率,用免疫组化、Western-Blot和ELISA方法分别检测VEGF基因转染MSCs后VEGF的表达情况。【结果】腺病毒介导的VEGF基因对于MSCs具有很高的转染效率,转染效率与病毒感染复制数(multiplicyties of ...
华平 +6 more
doaj +2 more sources
Zhongshan Daxue xuebao. Yixue kexue ban, 2004 【目的】 用流式细胞仪(FCM)快速检测外源性血管内皮生长因子基因(VEGF基因)在大鼠原代培养肝细胞转染表达,根据结果优化其转染表达条件?【方法】 以加强型黄色荧光素蛋白(EYFP)为标记,用FCM快速检测重组质粒pIRES-EYFP/VEGF121在大鼠原代培养肝细胞中转染表达,根据结果优化pIRES-EYFP/VEGF121转染表达条件?【结果】 pIRES-EYFP/VEGF121得以成功构建,并转染大鼠原代培养肝细胞;优化的转染表达条件:在细胞数密度0.1×106/mL,质粒孵育时间30 ...
doaj +2 more sources
Ultrasound in Obstetrics &Gynecology, Volume 60, Issue 4, Page 487-493, October 2022., 2022 ABSTRACT Objective To investigate the diagnostic yield of clinical whole‐genome sequencing (WGS) in prenatally diagnosed non‐immune hydrops fetalis (NIHF). Methods This was a retrospective study of 23 fetuses with prenatally diagnosed NIHF, negative for trisomies and copy‐number variants, referred for analysis by WGS with an in‐silico panel of 281 ...
E. Westenius +4 more
wiley +1 more source
Journal of Diabetes, Volume 14, Issue 4, Page 236-246, April 2022., 2022 Highlights Diabetic kidney disease is a progressive disease with the pathology of glomerular damage and loss of podocytes. Sodium glucose cotransporter 2 inhibitors (SGLT2i) can be expected to decrease the amount of podocyte excretion. Male patients receiving SGLT2i have better diabetic kidney disease‐related parameters and podocyturia levels. Abstract
Emre Durcan +13 more
wiley +1 more source
Veterinary Dermatology, Volume 32, Issue 4, Page 331-e92, August 2021., 2021 Background MicroRNAs (miRNA) are short, single‐stranded RNA molecules that regulate gene expression in a post‐transcriptional manner. Their expression is proposed to be tissue‐specific and alterations in miRNA expression have been detected in many diseases. Objective To compare miRNA expression in the skin of healthy Labrador and golden retrievers, and
Marie Isabel Morlang +3 more
wiley +1 more source
Journal of Diabetes, Volume 12, Issue 3, Page 224-236, March 2020., 2020 Highlights Diabetic conditions induce compositional dysbiosis of the gut microbiota and impair gut barrier integrity. Diabetic conditions markedly downregulate the abundance of Faecalibacterium prausnitzii in the gut. Under diabetic conditions, microbial anti‐inflammatory molecules from Faecalibacterium prausnitzii restore the gut barrier and ZO‐1 ...
Jihao Xu +7 more
wiley +1 more source
p38MAPK induces apoptosis of glioma cell [PDF]
, 2001 目的 研究 p38MAPK基因转染大鼠胶质瘤细胞系C6后对其生物学特性的影响 .方法 利用脂质体介导法将p38MAPK基因导入大鼠胶质瘤细胞系 C6中 ,用免疫细胞化学染色检测其在细胞转染前后的表达情况 ,用 HE染色、流式细胞仪等方法研究其对细胞形态、粘着状况和生长周期的影响 .结果 转染 p CMV5 - p38MAPK质粒组 p38MAPK蛋白表达阳性 ,细胞形态发生变化 ,贴壁性降低 ,出现大量凋亡细胞 .结论 转染 ...
叶菁(第四军医大学西京医院病理科) +6 more
core +3 more sources
Developmental Medicine &Child Neurology, Volume 61, Issue 3, Page E1-E35, March 2019., 2019 目的 本国际临床指南由欧洲残疾儿童学会（the European Academy of Childhood Disability，EACD）牵头制定，旨在解决发育性协调障碍（developmental coordination disorder，DCD）的定义、诊断、评估、干预以及与社会心理方面的临床应用关键问题。 方法 本指南针对五个领域的关键问题，通过文献综述和专家团队的正式讨论达成共识。为保证指南的循证基础，以“机制”、“评估”和“干预”为检索词, 对2012年更新以来提出的最新建议以及新增的“社会心理问题”和“青少年/成人”为检索词进行检索。根据牛津大学循证医学中心证据等级 (证据水平 [level of evidence, LOE]1–4) 将结果进行分类，最终转化为指南建议。并由国际 ...
Jing Hua +6 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2023 目的 通过基因沉默技术构建质粒成功转染原代星形胶质细胞(Ast)抑制IL-17表达，探讨IL-17的功能。方法 在制备原代Ast基础上，应用IL-17基因沉默质粒pGenesil-1A和B,用Lipofectamine 2000转染Ast,通过荧光分析法、RT-PCR法、Western Blotting法观察IL-17基因沉默后效果。结果 携带pGenesil-1B和A的IL-17基因沉默质粒制备成功后，免疫荧光携带pGenesil-1B转染效果明显高于pGenesil-1A,其转染率可高达56.72%;
沈笑然 +4 more
doaj
Comparative Proteomics Study on Human High-metastatic Large Cell Lung Cancer Cell Lines Before and After Transfecting with nm23-H1 Gene [PDF]
, 2010 Background and objective As a tumor metastasis suppressor gene, the functions of nm23-H1 gene are still unclear. The aim of this study is to better understand the mechanism of lung cancer metastasis and to find new biomarkers for early diagnosis and new ...
Li MA +6 more
core