Results 61 to 70 of about 15,062 (116)

用Psi2STK/GCV对鼠C6神经胶质瘤细胞作用的初步研究

Zhongguo shiyan zhenduanxue, 2005
目的研究利用基因工程包装细胞进行基因转导和药物治疗相结合来治疗脑胶质瘤的可行性。方法利用分子生物学方法构建了逆转录病毒载体STK及基因工程包装细胞psi2STK,用psi2STK细胞和丙氧鸟苷（GCV）结合对胶质瘤细胞进行了基因治疗研究。结果C6胶质瘤细胞和基因工程包装细胞与不同浓度的丙氧鸟苷（GCV）一起培养,未感染的细胞比感染的细胞对丙氧鸟苷（GCV）更为敏感。结论通过体外实验证实:逆转录病毒转导HSV-TK基因在培养C6胶质瘤细胞中,提高了其对GCV的敏感性。
梁淑媛, 于晓春, 周劲松, 于淑梅   +3 more
doaj  

[A case of neonatal fatty acid oxidation disorder]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Deng LL, Zou HM, Cheng TT, Yuan Y, Zhang HY.   +4 more
europepmc   +1 more source

IRAK-4基因沉默对人成骨样细胞凋亡的影响

Zhongshan Daxue xuebao. Yixue kexue ban, 2013
【目的】研究siRNA沉默白细胞介素-1受体相关激酶-4(IRAK-4)基因对人成骨样细胞株MG63凋亡及凋亡相关基因Bcl-2、Bax表达的影响。【方法】 以Lipofectamine 2000为载体将IRAK-4-siRNA转染入MG63细胞(CON组:不加入任何转染试剂;SC组:以scrambled siRNA序列进行转染;KD组:以特异性IRAK-4-siRNA序列进行转染),应用流式细胞术及TUNEL法检测细胞凋亡水平,western blotting检测Bcl-2、Bax基因的表达。【结果】
doaj  

[Expression of Rho-related BTB domain-containing protein 1 in tongue squamous cell carcinoma and its effect on cell proliferation, invasion, and migration]. [PDF]

Hua Xi Kou Qiang Yi Xue Za Zhi
Shao Y, Wang Y, Yu W, Xu Y, Xu J, Wang X, Chen Z.   +6 more
europepmc   +1 more source

[Efficacy of CD4/TGF-β bispecific antibody in a mouse model of peritoneal metastasis of malignant melanoma]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Jiang Q, Liu T, Wang Z, Jiang S, Wu H, Lin Y, Shiying LU, Chen L, Zhang J, Tang L, Raut RK, Xu Z.   +11 more
europepmc   +1 more source

Snail基因慢病毒载体的构建及其促进结肠癌细胞上皮间质化的研究

Zhongshan Daxue xuebao. Yixue kexue ban, 2012
【目的】 构建Snail基因慢病毒载体,研究Snail基因的表达与结肠癌细胞上皮间质化的关系。【方法】 利用Gateway Technology法构建Snail基因表达质粒和空白对照质粒,行慢病毒包装,成功构建Snail基因慢病毒载体后,在结肠癌细胞系caco2中转染Snail基因质粒及空白载体对照,构建稳转细胞株caco2- Snail和caco2-vc,并在LoVo细胞中瞬时转染上述质粒,对Snail及E-钙黏蛋白(E-cadherin)的表达进行检测,并用划痕 ...
doaj  

[Molecular characteristics for poor prognosis related renal cell carcinoma with lymph metastases]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Shu F, Ge L, Deng H, Yin H, Ou J, Deng S, Hao Y, Lu M, Zhang Z, Duan P, Zhang S.   +10 more
europepmc   +1 more source

[ZEB2 promotes glioblastoma progression by reprogramming lipid metabolism through the CYLD/FASN axis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Chen Z, Chai P, Mao Y, Liu R, Song Y.
europepmc   +1 more source

[Report of 24 cases of primary ciliary dyskinesia in children]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Feng XQ, Zhong LL, Peng L, Xu L, Zhou P.
europepmc   +1 more source

[Analysis of familial intrahepatic cholestasis caused by ubiquitin-specific peptidase 53 gene mutation]. [PDF]

Zhonghua Gan Zang Bing Za Zhi
Wang H, Zheng X, Yu MM, Yu ZD, Wang YS.
europepmc   +1 more source