Results 21 to 30 of about 14,404 (166)
Integrative Conservation, Volume 4, Issue 4, Page 665-674, December 2025.Using a diagnostic mitochondrial marker gene, we document the presence of furry‐eared dwarf lemurs, a species known from Madagascar's eastern montane rainforests, in several forest fragments across the island's central highlands. These findings raise new questions about when this species colonized these sites, how they tolerate these changing and ...
Marina B. Blanco +27 more
wiley +1 more source
Environmental DNA, Volume 7, Issue 4, July–August 2025.This study evaluates the effectiveness of three environmental DNA sampling approaches–water eDNA, air eDNA, and leech‐derived iDNA–for surveying vertebrate diversity in the Gaoligong Mountains of southwest China. Using five metabarcoding primer sets, we detected 235 vertebrate species.
Yunyu Wang +6 more
wiley +1 more source
TetraDENSITY 2.0—A Database of Population Density Estimates in Tetrapods
Global Ecology and Biogeography, Volume 33, Issue 12, December 2024.ABSTRACT Motivation Population density is a fundamental parameter in ecology and conservation, and taxonomic and geographic patterns of population density have been an important focus of macroecological research. However, population density data are time‐consuming and costly to collect, so their availability is limited.
L. Santini +10 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2019 目的为探讨mTOR信号通路在骨骼发育过程中的机制作用,建立骨骼发育相关的TSC1转基因小鼠,提供稳定的动物模型。方法取8周龄健康清洁级TSC1flox/flox小鼠分别与肢芽干细胞特异性重组酶(Prx-1-Cre)小鼠、软骨细胞特异性重组酶(Col2al-Cre)小鼠及成骨细胞特异性重组酶(Osx-Cre)小鼠进行杂交。将繁殖出的小鼠继续与TSC1flox/flox小鼠回交,并对其子代小鼠的基因型进行鉴定及mTOR活性检测。结果杂交后分别获得肢芽干细胞特异性TSC1敲除小鼠 ...
王红 +7 more
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Transgenic overexpression of miR-466a significantly disturbs OREBP/NFAT5 renal expression and induces phenotypes that resemble diabetes insipidus in human [PDF]
, 2012 目的miR-466(a/b/c/e)-5p/3pmiRNAs是两组高度保守的miRNAs，其中-5p组间和-3p组间miRNAs的序列几乎完全一样。生物信息学分析和我们的前期研究显示，miR-466miRNAs可能可以在肾组织中对渗透应激调控转录因子OREBP/NFAT5进行转录后表达调控。我们因此希望研究miR-466a在转基因小鼠中的过表达对小鼠肾组织OREBP/NFAT5的表达和小鼠尿液浓缩机制的影响。 方法通过PCR从小鼠基因组中扩增miR-466a基因序列，将片段插入鸡 ...
刘颖
core
An assessment of potential interventions to reduce the totoaba illegal trade market
Conservation Biology, Volume 38, Issue 5, October 2024.Abstract The illegal trade in totoaba (Totoaba macdonaldi) is causing adverse social, ecological, and economic impacts. This illegal activity is accelerating the overexploitation of totoaba and pushing the critically endangered vaquita (Phocoena sinus) closer to extinction.
Rodrigo Oyanedel +3 more
wiley +1 more source
2mol/L尿素溶血试验在UT-B基因敲除小鼠核型鉴定中的应用
Zhongguo shiyan zhenduanxue, 2007 目的探讨2 mol/L尿素溶血试验在UT-B基因敲除小鼠核型鉴定中的作用。方法（1）按SP级动物饲养标准进行饲养和繁殖;以UT-B基因敲除纯合子（UT-B-/-）成年雄鼠与C57/bl雌鼠合笼,繁殖出UT-B基因敲除杂合型小鼠（UT-B+/-,F1代）,F1代UT-B+/-雄、雌鼠交配获F2代小鼠。（2）取F2代小鼠剪尾提取基因组DNA进行核型鉴定筛选纯合子小鼠（UT-B-/-）和野生型小鼠（UT-B+/+）;（3）应用2 mol/L尿素溶血试验和肾脏RT-PCR进行UT-B-/-（JKnull ...
赵华山 +8 more
doaj
Toxic Effects and Mechanisms of Polychlorinated Biphenyls(Aroclor 1254) on Glucose Homeostasis in Male Mice [PDF]
, 2015 多氯联苯(PolychlorinatedBiphenyls,PCBs)作为最难以降解的持久性有机污染物之一，其引起的环境问题已经受到了极大的关注。有关环境污染物导致糖尿病的机制已经开展了较多的研究，但是关于环境水平的PCBs长期暴露能否导致糖尿病的发生以及其毒性效应机制还不清楚。本研究以C57BL/6品系青春期雄性小鼠为研究对象，研究环境水平的PCBs长期暴露导致糖尿病发生发展的毒性效应机制。采用浓度分别为0.5、5、50和500μg/kg的Aroclor1254，通过灌胃法对小鼠进行染毒 ...
张诗琦
core
Wildlife Letters, Volume 2, Issue 3, Page 131-139, September 2024.Southern flying squirrels (flying squirrel; Glaucomys volans) frequently displace endangered red‐cockaded woodpeckers (RCW; Dryobates borealis) within forest partitions managed specifically for RCW cavities (“cluster partitions”). We found little to no evidence of differences in home range size and composition between flying squirrels within or outside
Robert T. Meyer +2 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2016 目的观察肝原位瘤切除术对小鼠一般状态和免疫功能的影响。方法对小鼠进行肝原位瘤切除术,观察手术对小鼠术后不同时间节点自发活动度,淋巴细胞增殖能力,CD4+/CD8+比例等指标的影响。结果与正常小鼠相比,术后第1天小鼠自发活动度减小,差异有统计学意义 ...
赵彤 +8 more
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