Results 91 to 100 of about 1,473 (143)

[Design and synthesis of molecularly imprinted polymers and their applications in medical diagnosis and biocatalysis]. [PDF]

Se Pu
Song Q, Li X, Lyu Y.
europepmc   +1 more source

[Progresses on temporal interference electromagnetic stimulation for non-invasive deep brain function modulation]. [PDF]

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
Zhou T, Yu P, Xu Y.
europepmc   +1 more source

[Application and advances of exosome-hydrogel system in wound healing]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Qiu Y, Tan Q.
europepmc   +1 more source

[Establishment of an Epstein-Barr virus infection model using human nasal organoids]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Wei J, Zheng H, Zhao Y, Yu Y, Xu Y, Li G.   +5 more
europepmc   +1 more source

BPIFC基因真核表达载体的构建

Zhongguo shiyan zhenduanxue
目的 构建带有FLAG标签的BPIFC基因真核表达载体，建立稳定转染BPIFC基因的HaCaT细胞系。方法 设计引物通过PCR扩增出BPIFC基因片段，利用DNA重组技术将该基因片段定向插入pcDNA3.1-flag-N真核表达载体中，并对重组质粒进行双酶切及DNA测序鉴定。验证正确的重组质粒通过PI Max线性转染的方法转染至HaCaT细胞，通过G418筛选建立BPIFC稳定转染的HaCaT细胞系，并通过RT-PCR法和Western-blot法分别测定BPIFC mRNA和蛋白的表达量。结果 ...
陆娇娇, 郑娴, 董文婿, 杨菁, 曹罗元, 富显果   +5 more
doaj  

[Gandou Bushen decoction ameliorates ovarian injury in murine hepatolenticular degeneration via the AGE/RAGE/NF-κB signaling pathway]. [PDF]

Zhejiang Da Xue Xue Bao Yi Xue Ban
Jiang P, Wu L, Han H.
europepmc   +1 more source

[Transcriptomic characteristics of cervical endplate cartilage in cervical spondylosis and effects of acupotomy on the FGF18/Akt axis with cervical spondylosis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Liu F, Khaliunaa T, Cao Q, Yang Y, Ren C, Zhu J, Zhao X, Cao L, Deng B, Wang X.   +9 more
europepmc   +1 more source

[Retrospect and Prospect of Immune Caries Prevention Research in China]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Xu Q, Fan M.
europepmc   +1 more source

人神经生长因子基因真核细胞表达载体的构建与鉴定

Zhongshan Daxue xuebao. Yixue kexue ban, 1999
目的: 构建携带人神经生长因子( hNGF)基因的真核细胞表达载体, 为应用 NGF 进行老年性痴呆(AD)等疾病基 因治疗打基础。方法: 应用基因重组技术将 hNGF cDNA 克隆到逆转录病毒载体 pLXSN 中 ,用限制性内切酶酶切分析重组质粒 pLNGFSN 中 hNGF 基因的插入方向,用 PCR、斑点杂交和 Southern 杂交对重组质粒 pLNGFSN 作进一步鉴定。结果: hNGF cDNA 已正确地克隆到逆转录病毒载体 pLXSN 中,而构建成重组逆转录病毒载体 pLNGFSN ...
doaj  

[Efficient separation of gossypol from cotton kernels with copper mediated magnetic molecularly imprinted polymer]. [PDF]

Se Pu
Yang SL, Cao Y, He KL, Feng S, Zhang CG, Shan LH.   +5 more
europepmc   +1 more source