Results 91 to 100 of about 1,252 (143)

[Transcriptomic characteristics of cervical endplate cartilage in cervical spondylosis and effects of acupotomy on the FGF18/Akt axis with cervical spondylosis]. [PDF]

open access: yesNan Fang Yi Ke Da Xue Xue Bao
Liu F   +9 more
europepmc   +1 more source

[Therapeutic potential of extracellular vesicles in neurological diseases]. [PDF]

open access: yesNan Fang Yi Ke Da Xue Xue Bao
DU Q, Yang C, Xia X, Wang T.
europepmc   +1 more source

BPIFC基因真核表达载体的构建

open access: yesZhongguo shiyan zhenduanxue
目的 构建带有FLAG标签的BPIFC基因真核表达载体,建立稳定转染BPIFC基因的HaCaT细胞系。方法 设计引物通过PCR扩增出BPIFC基因片段,利用DNA重组技术将该基因片段定向插入pcDNA3.1-flag-N真核表达载体中,并对重组质粒进行双酶切及DNA测序鉴定。验证正确的重组质粒通过PI Max线性转染的方法转染至HaCaT细胞,通过G418筛选建立BPIFC稳定转染的HaCaT细胞系,并通过RT-PCR法和Western-blot法分别测定BPIFC mRNA和蛋白的表达量。结果 ...
陆娇娇   +5 more
doaj  

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