Results 31 to 40 of about 991,523 (156)
Rare Metals, Volume 45, Issue 3, March 2026.ABSTRACT Platinum (Pt)‐based catalysts are crucial for the commercialization of hydrogen/metal air batteries, but they suffer from Pt scarcity, high cost, and nanoparticle dissolution/agglomeration, mainly leading to performance degradation. We address this by synthesizing a PtFe alloy catalyst (PtFe–PA–NC) via a phytic acid (PA) assisted pyrolysis ...
Chenyang Shu +9 more
wiley +1 more source
靶向人类免疫缺陷病毒1型(HIV-1)整合酶多肽药物表达载体的构建 [PDF]
, 2019 目的构建人类免疫缺陷病毒1型(HIV-1)整合酶(IN)表达载体用于双分子荧光互补技术筛选多肽药物。方法PCR扩增HIV-1 IN的全长基因序列,将目的片段插入至pBiFc-VN173载体中,并对重组质粒pBiFc-VN173-IN进行双酶切及测序鉴定。将重组质粒pBiFc-VN173-IN和对照组质粒pBiFc-VN173分别转染HEK293T细胞,采用Western blot法和免疫荧光细胞化学染色法检测IN的表达。结果通过高保真PCR、载体构建和鉴定,成功获得IN表达载体pBiFc-VN173 ...
魏碧娜 +5 more
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Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT The poor Zn chemistry of Zn metal anode and sluggish reaction kinetics of cathodes at high mass loadings are two crucial issues for hindering the application of aqueous Zn‐ion batteries (AZIBs). Herein, the hierarchical porous N‐doped carbon fibers (HPNCFs) were proposed to simultaneously optimize the reversibility of Zn plating/stripping of ...
Song Yang +6 more
wiley +1 more source
Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT Intervertebral disc degeneration (IDD) is a predominant cause of low back pain, significantly impacting quality of life. Mitochondrial fitness is essential in the progression of IDD, as it regulates cellular senescence and pyroptosis. This study investigates the role of mitochondrial caseinolytic protease P (ClpP) in alleviating senescence and
Kun Wang +7 more
wiley +1 more source
Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT Photodetectors, based on GaAs nanowires (NWs), hold significant promise in the fields of high integration micro‐ and nano‐optoelectronics applications because of their outstanding electronic and optical properties. To date, significant efforts have been directed toward enhancing the performance of photodetectors, aiming for high detectivity ...
Yu Hao +11 more
wiley +1 more source
Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT The sluggish kinetics of the oxygen reduction reaction (ORR) necessitate developing oxygen electrodes with enhanced electrocatalytic activity for practical zinc–air battery applications. In this study, an iron‐doped copper sulfide electrocatalyst (CuFeNS‐PNC) anchored on metal–organic framework‐derived carbon substrates was designed, featuring
Taichong Chitboonyakasem +4 more
wiley +1 more source
Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT Regulating negative parameters of metacomposites is mainly limited by their unclear mechanisms, which affects their practical applications in new sensing, communication, electromagnetic (EM) protection and other fields. This work proposes a dual‐functional competitive design strategy based on the waste corncob‐derived carbon/polyaniline ...
Qiuyun Yang +4 more
wiley +1 more source
, 2005 从籼稻川75A(OryzasativaL.)黄化苗叶片中直接提取基因组总DNA.根据文献报道的水稻花药绒毡层特异启动子Osg6B序列设计引物,采用TouchdownPCR技术获得水稻花药绒毡层特异表达的启动子Tsp1,将该片段克隆在载体pMD18-TVector上转化到大肠杆菌JM109,并酶切和PCR验证.序列分析发现,该片段与Osg6B启动子同源性为96%,且具有真核生物启动子的多种特征序列,表明启动子Tsp1为来源于籼稻川75A中的水稻花药绒毡层特异表达的启动子 ...
陈荣军 +4 more
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Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT Harnessing solar energy for self‐heating presents an effective strategy to suppress surface ice formation. In this paper, we fabricated nano‐silver‐modified multilayer graphene sheets (Ag@MGs) that integrate photothermal and superhydrophobic properties for anti‐icing applications.
Yihan Zhang +7 more
wiley +1 more source
, 2003 目的 运用植物转基因技术 ,探索构建表达人类 β-防御素 - 2 ( h BD- 2 )的植物生物反应器的可能性及技术路线。方法 将 C端带 myc和 6x His双标记的重组 h BD- 2基因插入植物真核表达载体 p CAMBIA13 0 4中 ,构建 C端带 m yc和 6x His双标记的重组 h BD- 2植物真核表达载体 rp CAMBIA13 0 4/ h BD- 2 / His。利用此载体转化根癌农杆菌 L BA44 0 4,经卡那霉素进行抗性基因筛选和 PCR检测 ...
杜军 +5 more
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