Macrophage differential genes and their functions in silicosis based on single cell RNA sequencing and spatial transcriptome sequencing [PDF]
, 2022 BackgroundThe rise of single cell RNA sequencing (scRNA-seq) and spatial transcriptome sequencing technologies has allowed for intensive study of lung diseases, but both have been poorly studied in silicosis.
Jie CHAO, Wei ZHANG, Xinbei ZHOU
core +2 more sources
Transcriptome Sequencing Analysis of Chrysomyia Megacephala Pupae in Different Growing Periods [PDF]
, 2021 Objective To study the growth regulation, environmental adaption and epigenetic regulation of Chrysomyia Megacephala pupae, in order to obtain the transcriptome data of Chrysomyia Megacephala in different growing periods, and lay the foundation for ...
WANG Qi-yan, ZHANG Hong-ling, REN Zheng, et al.
core +1 more source
Transcriptomic Analysis and Products of Nutrient Transformation in Hongjundouzha, a Traditional Hakka Fermented Okara Food Fermented by Neurospora crassa LY03, during Fermentation [PDF]
, 2023 In this study, the nutritional components of Hongjundouzha inoculated with Neurospora crassa at different fermentation times were analyzed. RNA sequencing (RNA-seq), quantitative real-time polymerase chain reaction (real-time PCR) and ultra-high ...
LIN Biaosheng, CHEN Guanxi, ZHANG Qingji, LIN Bin, CHEN Xiaohong, LI Ying
core +1 more source
Research Progress of Functional Enzymes inTraditional Fermented Food [PDF]
, 2023 Traditional fermented foods could produce a variety of functional enzymes and plenty of flavor compounds through microbial fermentation. The production process of fermented foods would be constantly enriched with abundant enzymes, and it is the current ...
Hai ZHAO +4 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2023 目的 探讨转录因子E2F3在上皮性卵巢癌(EOC)中的表达及临床意义。方法 在癌症基因组图谱(TCGA)数据库及基因表达数据库(GEO)中分析E2F家族成员在EOC组织与正常组织中的表达差异及临床意义，同时借助生物信息数据库预测E2F3的转录调控靶点。应用RT-qPCR和Western blot检测EOC及对照组中转录因子E2F3及其靶基因的mRNA及蛋白表达水平。结果 TCGA数据信息结合GEO测序结果提示，与对照组相比在EOC中E2F1、E2F3呈现显著高表达 ...
张晶, 孔丽丽
doaj
Genome Assembly and Annotation of a High-Polymalic Acid (PMLA) Producing Strain Aureobasidium melanogenum CGMCC18996 and Analysis of Its Key Proteins Related to PMLA Synthesis [PDF]
, 2023 In this study, we applied PacBio Sequel II and Illumina NovaSeq 6000 sequencing platforms to sequence the genome of a high-polymalic acid (PMLA)-producing strain, Aureobasidium melanogenum CGMCC18996, and used different assemblers to obtain a high ...
WANG Genan, LI Jiaqian, LI Yutong, CHEN Shiwei, WANG Shuxian, ZHAO Tingbin, JIA Shiru, QIAO Changsheng
core +1 more source
基于RNA-Seq技术的野生和圈养塔里木马鹿皮肤组织转录组比较分析
野生动物学报, 2023 为探寻野生和圈养塔里木马鹿（Cervus elaphus yarkandensis）的基因表达谱是否存在差异，并进一步筛选该物种适应干旱环境的相关基因，利用RNA-Seq技术对野生和圈养塔里木马鹿的皮肤组织进行转录组测序，对测序数据质控后比对参考基因组，筛选差异表达基因并对其进行功能注释和富集分析，利用qRT-PCR方法对转录组测序结果进行可靠性检测。结果显示：与圈养塔里木马鹿相比，在野生塔里木马鹿皮肤组织中共有3 303个差异表达基因，包括上调表达基因1 535个，下调表达基因1 768个 ...
布威海丽且姆·阿巴拜科日 +3 more
doaj
The role and mechanism of hnRNP A2/B1 in breast cancer [PDF]
, 2015 核不均一核糖核蛋白A2/B1（hnRNPA2/B1）是hnRNP蛋白家族成员，在肿瘤细胞增殖与凋亡过程中具有重要作用。本实验为了探究hnRNPA2/B1蛋白在乳腺癌组织中的表达及其在乳腺癌细胞中的定位。研究hnRNPA2/B1在乳腺癌细胞中所涉及的主要功能、信号通路以及可变剪接和蛋白融合过程，并探究hnRNPA2/B1对细胞增殖、凋亡、迁移等生物功能的影响。从而阐明hnRNPA2/B1在乳腺癌中的作用机制，为肿瘤的机理研究、临床诊断和治疗提供一个新靶点。本实验用免疫组化方法检测hnRNPA2 ...
韩荣
core
comparative analysis of Litopenaeus vannanmei by transcriptome sequencing and characterization of two immune-relevant genes [PDF]
, 2015 南美白对虾是目前对虾养殖的主要品种，具有巨大的经济价值。然而，病害的发生严重危害其养殖业的发展，研究对虾的先天免疫反应能够给对虾养殖中病害的防治提供重要的理论基础。 转录组测序能够分析某一生理条件下细胞内所有mRNA的表达水平，本研究通过二代测序技术首次比较分析了对WSSV（whitespotsyndromevirus）抗性差异的两种南美白对虾群体的转录组，它们的基因表达水平存在明显差异，具有750个明显差异表达的基因，普通对虾及抗病对虾群体在病毒感染前后分别有479和132个差异表达基因 ...
徐景祥
core
Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress [PDF]
Cryptolestes ferrugineus, a cosmopolitan stored-grain pest, was studied using transcriptome sequencing to investigate its molecular response to low-temperature stress..
FANG Zhi-yi +5 more
core +1 more source