Results 1 to 10 of about 6,100 (106)

Single-cell RNA-sequencing-guided reactive oxygen species-scavenging hydrogel design for regeneration of osteoporotic bone. [PDF]

J Zhejiang Univ Sci B
Hu W, Fei T, Liu Z, Ding Y, Yu M, Yang F, Zhao F.   +6 more
europepmc   +1 more source

[Revealing research progress for immunological characteristics of chronic hepatitis B applying single-cell RNA sequencing technology]. [PDF]

Zhonghua Gan Zang Bing Za Zhi
Shu C, Zhang DZ.
europepmc   +1 more source

高原低氧环境抑制小鼠脾脏组织碳代谢的分子机制

Zhongshan Daxue xuebao. Yixue kexue ban
目的探究低氧暴露对小鼠脾脏组织碳代谢通路的调控机制。方法将C57BL/6小鼠分别在海拔400 m和4 200 m饲养，每组5只，30 d后无菌取出脾脏组织，通过转录组测序，蛋白质组学和非靶向代谢组学分析差异表达基因、差异蛋白以及差异代谢物，利用GO和KEGG富集分析，挖掘关键通路，并通过RT-qPCR和Western blot对通路中关键基因和蛋白进行验证。结果转录组测序发现，低氧暴露下有4 213个基因出现显著性差异表达，其中1 947个基因表达上调，2 266个基因表达下调 ...
陈晓晨, 胡英, 许玉珍, 龙启福, 马茹雪, 永胜   +5 more
doaj  

[Detection of molecular affecting sensitivity to local glucocorticoid therapy in oral lichen planus through transcriptome sequencing]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Ren X, Li K, Li C.
europepmc   +1 more source

马鹿MORF4L2组织表达、基因克隆及生物信息学分析

野生动物学报
MORF4L2是一种转录因子，通过形成NuA4组蛋白乙酰转移酶复合物来参与异染色质组装和组蛋白修饰，对细胞生长、增殖和凋亡起重要作用。为了探究马鹿（Cervus elaphus）鹿茸中MORF4L2所发挥的功能，使用qPCR技术检测鹿茸不同组织中MORF4L2基因的表达水平，采用PCR克隆马鹿MORF4L2基因的CDS序列，通过多物种比对MORF4L2基因mRNA序列进行相似性分析并构建系统进化树，利用生物信息学方法预测分析MORF4L2编码蛋白的结构与理化性质。结果显示 ...
韩青, 高仰, 吴玄烨, 索婧媛, 金庆梅, 刘学东   +5 more
doaj  

Anti-inflammatory effects and related mechanisms of naringenin in human periodontal ligament stem cells under lipopolysaccharide stimulation based on RNA sequencing. [PDF]

Hua Xi Kou Qiang Yi Xue Za Zhi
Li J, Xu X, Liu X, Zeng T, Zhang L, Zheng Q.   +5 more
europepmc   +1 more source

PGC-1α辅助激活人PEPCK基因转录

Zhongshan Daxue xuebao. Yixue kexue ban, 2011
【目的】构建人磷酸烯醇式丙酮酸羧激酶(PEPCK)基因启动子荧光素酶报告质粒PGL3-hPCK-luc，并探讨过氧化物酶体增殖物活化受体γ辅助激活因子1α(PGC-1α)在转录因子肝细胞核因子4α(HNF4α)介导下对人PEPCK基因启动子活性的影响【方法】从人全血基因组DNA中克隆PEPCK启动子基因片段，并重组进荧光素酶报告载体PGL3-basic，将pcDNA3.1-PGC-1αpcDNA3.0-HNF4α表达质粒与PGL3-hPCK ...
doaj  

[ZEB2 promotes glioblastoma progression by reprogramming lipid metabolism through the CYLD/FASN axis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Chen Z, Chai P, Mao Y, Liu R, Song Y.
europepmc   +1 more source

[Expression profile of intervertebral disc degeneration-specific genes: a transcriptome sequencing-based analysis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao, 2021
Xia B, Xing J, Ai Q, Li H, Xu M, Hou T.
europepmc   +1 more source

[Effect of LncRNA DANCR on the immune microenvironment of glioma cells by regulating the miR-656/BMPR1A axis]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Wang O, Zhu P, Lin J, Wu H.
europepmc   +1 more source