Role of 16‐S RNA in Ribosome Messenger Recognition [PDF]
The deoxyoctanucleotide (5′‐3′)d(A‐A‐G‐G‐A‐G‐G‐T), which is complementary to the 3′ end of 16‐S RNA, inhibits the formation of the complex between the 30‐S subunit and MS2 RNA described in the preceding paper. If the complex is preformed, the octanucleotide cannot prevent entry of the complex into the ribosome cycle upon supplementation with the ...
Jan van Duin+5 more
openaire +3 more sources
A model for the study of ligand binding to the ribosomal RNA helix h44. [PDF]
Oligonucleotide models of ribosomal RNA domains are powerful tools to study the binding and molecular recognition of antibiotics that interfere with bacterial translation.
Dibrov, Sergey M+2 more
core +3 more sources
Recognition of Ribosomal RNA Sites in DNA, I. Analysis of the E. coli System [PDF]
The evidence recently presented of specific hybridization between bacterial ribosomal RNA and homologous DNA1-3 has indicated the possibility of a biochemical approach to the problem of the identification of ribosomal RNA sites in DNA.
Attardi, Giuseppe+2 more
core +1 more source
Ribosomal Protein S12 Hastens Nucleation of Co-Transcriptional Ribosome Assembly
Ribosomal subunits begin assembly during transcription of the ribosomal RNA (rRNA), when the rRNA begins to fold and associate with ribosomal proteins (RPs). In bacteria, the first steps of ribosome assembly depend upon recognition of the properly folded
Margaret L. Rodgers+2 more
doaj +1 more source
Location of Ribosomal Protein Binding Sites on 16S Ribosomal RNA [PDF]
The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of individual proteins with RNA fragments purified from partial enzymatic digests. Of the six 30S subunit proteins known to interact directly with
Chantal Ehresmann+4 more
openaire +3 more sources
Aptazyme-Mediated Regulation of 16S Ribosomal RNA
Developing artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli.
Wieland, Markus+3 more
openaire +4 more sources
Mutations in 16S ribosomal RNA disrupt antibiotic-RNA interactions. [PDF]
Two of six mutations at a base-paired site in Escherichia coli 16S rRNA confer resistance to nine different aminoglycoside antibiotics in vivo. Chemical probing of mutant and wild-type ribosomes in the presence of paromomycin indicates that interactions between the antibiotic and 16S rRNA in mutant ribosomes are disrupted.
Danesh Moazed+3 more
openaire +2 more sources
Action spectra for UV-light induced RNA–RNA crosslinking in 16S ribosomal RNA in the ribosome [PDF]
UV irradiation induces intramolecular crosslinks in ribosomal RNA in the ribosome. These crosslinks occur between nucleotides distant in primary sequence and they are specific, limited in number and have crosslinking efficiencies sufficient to allow their use in monitoring conformational changes.
Oksana V. Zhirnov, Paul Wollenzien
openaire +3 more sources
Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]
In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini+4 more
core +3 more sources
The 16S ribosomal RNA mutation database (16SMDB) [PDF]
The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenotype has been detected by in vivo or ...
openaire +5 more sources