Results 31 to 40 of about 134,692 (313)
Selection of reference genes for expression analysis using quantitative real-time PCR in the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera, Aphidiae). [PDF]
PLoS ONE, 2014 To facilitate gene expression study and obtain accurate qRT-PCR analysis, normalization relative to stable expressed housekeeping genes is required. In this study, expression profiles of 11 candidate reference genes, including actin (Actin), elongation ...
Chunxiao Yang +3 more
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The structure of a methylated tetraloop in 16S ribosomal RNA [PDF]
Structure, 1998 Ribosomal RNAs contain many modified nucleotides. The functions of these nucleotides are poorly understood and few of them are strongly conserved. The final stem loop in 16S-like rRNAs is an exception in both regards. In both prokaryotes and eukaryotes, the tetranucleotide loop that caps the 3'-terminal stem contains two N6, N6-dimethyladenosine ...
Jason P. Rife, Peter B. Moore
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Parasites & Vectors, 2020 Background Molecular advances have accelerated our understanding of nematode systematics and taxonomy. However, comparative analyzes between various genetic markers have led to discrepancies in nematode phylogenies.
Abigail Hui En Chan +4 more
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Functional Modification of 16S Ribosomal RNA by Kethoxal [PDF]
Proceedings of the National Academy of Sciences, 1972 Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since ( a ) loss of specific binding of transfer RNA, but not binding of ...
Jonathan B. Chaires, Harry F. Noller
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Role of 16‐S RNA in Ribosome Messenger Recognition [PDF]
European Journal of Biochemistry, 1980 The deoxyoctanucleotide (5′‐3′)d(A‐A‐G‐G‐A‐G‐G‐T), which is complementary to the 3′ end of 16‐S RNA, inhibits the formation of the complex between the 30‐S subunit and MS2 RNA described in the preceding paper. If the complex is preformed, the octanucleotide cannot prevent entry of the complex into the ribosome cycle upon supplementation with the ...
Jan van Duin +5 more
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Nucleic Acids Research, 2012 16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
A. Klindworth +6 more
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Ribosomal Protein S12 Hastens Nucleation of Co-Transcriptional Ribosome Assembly
Biomolecules, 2023 Ribosomal subunits begin assembly during transcription of the ribosomal RNA (rRNA), when the rRNA begins to fold and associate with ribosomal proteins (RPs). In bacteria, the first steps of ribosome assembly depend upon recognition of the properly folded
Margaret L. Rodgers +2 more
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Mutations in 16S ribosomal RNA disrupt antibiotic-RNA interactions. [PDF]
The EMBO Journal, 1989 Two of six mutations at a base-paired site in Escherichia coli 16S rRNA confer resistance to nine different aminoglycoside antibiotics in vivo. Chemical probing of mutant and wild-type ribosomes in the presence of paromomycin indicates that interactions between the antibiotic and 16S rRNA in mutant ribosomes are disrupted.
Danesh Moazed +3 more
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Action spectra for UV-light induced RNA–RNA crosslinking in 16S ribosomal RNA in the ribosome [PDF]
Photochemical & Photobiological Sciences, 2003 UV irradiation induces intramolecular crosslinks in ribosomal RNA in the ribosome. These crosslinks occur between nucleotides distant in primary sequence and they are specific, limited in number and have crosslinking efficiencies sufficient to allow their use in monitoring conformational changes.
Oksana V. Zhirnov, Paul Wollenzien
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The 16S ribosomal RNA mutation database (16SMDB) [PDF]
Nucleic Acids Research, 1994 The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenotype has been detected by in vivo or ...
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