Phylogenetic relationships of amphibian families inferred from DNA sequences of mitochondrial 12S and 16S ribosomal RNA genes. [PDF]
Jennifer M. Hay +3 more
openalex +2 more sources
Selection of reference genes for expression analysis using quantitative real-time PCR in the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera, Aphidiae). [PDF]
To facilitate gene expression study and obtain accurate qRT-PCR analysis, normalization relative to stable expressed housekeeping genes is required. In this study, expression profiles of 11 candidate reference genes, including actin (Actin), elongation ...
Chunxiao Yang +3 more
doaj +1 more source
Functional Modification of 16S Ribosomal RNA by Kethoxal [PDF]
Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since ( a ) loss of specific binding of transfer RNA, but not binding of ...
H F, Noller, J B, Chaires
openaire +2 more sources
Role of 16‐S RNA in Ribosome Messenger Recognition [PDF]
The deoxyoctanucleotide (5′‐3′)d(A‐A‐G‐G‐A‐G‐G‐T), which is complementary to the 3′ end of 16‐S RNA, inhibits the formation of the complex between the 30‐S subunit and MS2 RNA described in the preceding paper. If the complex is preformed, the octanucleotide cannot prevent entry of the complex into the ribosome cycle upon supplementation with the ...
C, Backendorf +5 more
openaire +2 more sources
Background Molecular advances have accelerated our understanding of nematode systematics and taxonomy. However, comparative analyzes between various genetic markers have led to discrepancies in nematode phylogenies.
Abigail Hui En Chan +4 more
doaj +1 more source
Erratum in: 16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis. [PDF]
europepmc +3 more sources
A model for the study of ligand binding to the ribosomal RNA helix h44. [PDF]
Oligonucleotide models of ribosomal RNA domains are powerful tools to study the binding and molecular recognition of antibiotics that interfere with bacterial translation.
Dibrov, Sergey M +2 more
core +3 more sources
The structure of a methylated tetraloop in 16S ribosomal RNA [PDF]
Ribosomal RNAs contain many modified nucleotides. The functions of these nucleotides are poorly understood and few of them are strongly conserved. The final stem loop in 16S-like rRNAs is an exception in both regards. In both prokaryotes and eukaryotes, the tetranucleotide loop that caps the 3'-terminal stem contains two N6, N6-dimethyladenosine ...
Rife, Jason P, Moore, Peter B
openaire +2 more sources
Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]
In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini +4 more
core +3 more sources
Location of Ribosomal Protein Binding Sites on 16S Ribosomal RNA [PDF]
The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of individual proteins with RNA fragments purified from partial enzymatic digests. Of the six 30S subunit proteins known to interact directly with
R A, Zimmermann +4 more
openaire +2 more sources

