Results 91 to 100 of about 1,076,866 (305)

Rab1A Promotes Hepatic Steatosis by Suppressing Mitophagy via the Raf‐1/ERK1/2/PINK1 Signaling Axis

Advanced Science, EarlyView.
Why does fat accumulate in the liver? Our study reveals Rab1A as the molecular switch that silences the cell's mitochondrial cleanup crew. Turning Rab1A off reactivates mitophagy, clears excess fat, and reverses fatty liver disease in mice—offering a promising new therapeutic target for MASLD, a growing global health challenge.
Li Zhang, Jianhua Li, Huilu Zhang, Qi Qin, Yichen Huang, Xiaofan Tian, Chao Sun, Binbin Li, Zhengxin Wang, Xin Zhang   +9 more
wiley   +1 more source

Accurate Identification of Common Pathogenic Nocardia Species: Evaluation of a Multilocus Sequence Analysis Platform and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. [PDF]

PLoS ONE, 2016
Species identification of Nocardia is not straightforward due to rapidly evolving taxonomy, insufficient discriminatory power of conventional phenotypic methods and also of single gene locus analysis including 16S rRNA gene sequencing.
Meng Xiao, Lu Pang, Sharon C-A Chen, Xin Fan, Li Zhang, Hai-Xia Li, Xin Hou, Jing-Wei Cheng, Fanrong Kong, Yu-Pei Zhao, Ying-Chun Xu   +10 more
doaj   +1 more source

16S rRNA Library Preparation and Sequencing

Abstract This chapter describes the targeting of the prokaryotic 16S ribosomal RNA gene (16S rRNA) using both short-read Illumina Sequencing and long-read Nanopore Sequencing. 16S rRNA targets can be present in multiple copies across the genome and are another consideration when trying to quantitatively determine the proportion of ...
Dave Baker, Cara–Jane Moss
openaire   +1 more source

Removing noise from pyrosequenced amplicons [PDF]

, 2011
<b>Background</b><p></p> In many environmental genomics applications a homologous region of DNA from a diverse sample is first amplified by PCR and then sequenced.
Quince, Christopher, Marie Ollier, Decousus, Stephanie, Davenport, Russell J., Christopher Quince, Davenport Russell J, Quince Christopher, Ollier, Marie, Sandrine Viala, Uhrhammer, Nancy, Saghir, Nagi, S El, Turnbaugh, Peter, Davenport, Russell J, Turnbaugh, Peter J., Lanzen Anders, Ponelle, Flora, Nancy Uhrhammer, Russell J Davenport, Lanzen, Anders, Uhrhammer, Nancy A., Axel Poulet, Privat, Maud, Peter J Turnbaugh, Maud Privat, Axel Perin, Lafarge, Laurence, Bidet, Yannick, Laurence Lafarge, Stephanie Decousus, Poulet, Axel, Viala, Sandrine, Yannick Bidet, Nagi S. El Saghir, Yves-Jean Bignon, Bignon, Yves-Jean, Turnbaugh Peter J, El-Saghir, Nagi S., Flora Ponelle, Anders Lanzen, Perin, Axel, Bignon, Yves Jean   +40 more
core   +1 more source

Full-length 16S rRNA gene amplicon analysis using nanopore sequencing

, 2023
Nanopore sequencing reads of 16S rRNA gene amplicons from the 10-species mock bacterial community (ATCC MSA-1000).
Yoshiyuki Matsuo (12493648)
core   +1 more source

Pre‐Regenerative Endothelial Cells Empowered by Antler Blood Exosomes Orchestrate Peripheral Nerve Repair

Advanced Science, EarlyView.
The pre‐regenerative vascular niche (PVN) is essential for nerve repair, yet its endothelial blueprint remains unclear. We identified angiogenic ECs (AECs) as the dominant pre‐regenerative subset and found that antler blood–derived exosomes (AB‐EXO) promote repair via IMP3.
Jinsheng Huang, Qiang Chen, Aihua Jin, Xiaoqi Yang, Hui Zhou, Weiquan Gong, Jin Cheng, Jun Dai, Minjin Wang, Meihua Piao, Yuanyi Wang, Nan Zhou   +11 more
wiley   +1 more source

Rapid and reliable species-level identification from clinical samples using 16S rRNA gene nanopore sequencing analysis

Scientific Reports
The detection and identification of bacterial species in clinical samples are crucial for patient management and antibiotic treatment. When culture-based identification methods fail, 16S rRNA gene next-generation sequencing (NGS) serves as a valuable ...
Marco J. R. Ivens, Sergio Kamminga, Kawtar Benchamach, Chaimae Akile, Els Wessels, Eric C. J. Claas, Stefan A. Boers   +6 more
doaj   +1 more source

Bacterial phylogeny based on 16S and 23S rRNA sequence analysis [PDF]

FEMS Microbiology Reviews, 1994
Molecular phylogeny increasingly supports the understanding of organismal relationships and provides the basis for the classification of microorganisms according to their natural affiliations. Comparative sequence analysis of ribosomal RNAs or the corresponding genes currently is the most widely used approach for the reconstruction of microbial ...
W, Ludwig, K H, Schleifer
openaire   +2 more sources

Lactiplantibacillus Plantarum GUANKE Alleviates Allergic Asthma by Restricting Monocyte‐to‐DC Differentiation Via Microbiota‐Associated IAA

Advanced Science, EarlyView.
Lactiplantibacillus plantarum GUANKE remodels the indigenous gut microbiota and is associated with microbiota‐dependent restoration of circulating IAA. IAA treatment attenuates JAK3‐STAT3 and Jag1‐Notch4‐Hey1 signaling and reduces CX3CR1+Ly6C− monocytic precursor generation and pulmonary CD11b+ DC accumulation, together with reduced Th2 inflammation ...
Yujia He, Jielan Mi, Zhiping Zhao, Xiao Zhang, Zhihan Yang, Kun Yue, Yuanming Huang, Liqiong Song, Xiaoting Wen, Jianguo Xu, Zhihong Ren   +10 more
wiley   +1 more source

Vibrio 16S rRNA gene sequences

This database includes 16S rRNA gene sequences of complete Vibrio genomes from RefSeq (51 species, 317 strains - including 22 V. vulnificus strains), 41 draft V. vulnificus genomes from clinical isolates from the Baltic Sea region, and 84 draft V. vulnificus genomes from environmental Baltic Sea isolates.
Delgado, Luis Fernando, Labrenz, Matthias, Andersson, Anders F.   +2 more
openaire   +2 more sources