Results 111 to 120 of about 1,076,866 (305)

Development of a reference data set for assigning Streptococcus and Enterococcus species based on next generation sequencing of the 16S–23S rRNA region

Antimicrobial Resistance and Infection Control, 2019
Background Many members of Streptococcus and Enterococcus genera are clinically relevant opportunistic pathogens warranting accurate and rapid identification for targeted therapy.
Maja Kosecka-Strojek, Artur J. Sabat, Viktoria Akkerboom, Anna M. D. (Mirjam) Kooistra-Smid, Jacek Miedzobrodzki, Alexander W. Friedrich   +5 more
doaj   +1 more source

Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing

The Journal of General and Applied Microbiology, 2015
In this study, the picocyanobacterial species composition of Lake Miyagase was examined by analyzing the 16S rRNA gene in a clone library and by amplicon sequencing using a benchtop next-generation sequencer. Five separate samples were analyzed from different days over a ten-month period. In the picocyanobacterial lineage, 9 and 12 OTUs were identified
Fujimoto, Naoshi, Mizuno, Keigo, Yokoyama, Tomoki, Ohnishi, Akihiro, Suzuki, Masaharu, Watanabe, Satoru, Komatsu, Kenji, Sakata, Yoichi, Kishida, Naohiro, Akiba, Michihiro, Matsukura, Satoko   +10 more
openaire   +3 more sources

Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences [PDF]

, 2001
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Jong, S., de, Kowalchuk, G.A., Stephen, J.R., Speksnijder, A.G.C.L., Jong, S. de, Kline, E., Laanbroek, H.J.   +6 more
core   +1 more source

Culture-enriched 16S rRNA gene sequencing workflow.

, 2023
Cecal samples were collected from horses. One aliquot was submitted for direct 16S rRNA sequencing. Other aliquots were plated onto 6 different media types and incubated anaerobically and anaerobically.
Luis G. Arroyo (14797307), Patrick Boerlin (487065), Diego E. Gomez (5954507), Benjamin B. Caddey (15285404), Luiza S. Zakia (15285401), Michael G. Surette (8603931)   +5 more
core   +1 more source

Lactobacillus‐Loaded Bioadhesive Hydrogel with Prolonged Retention and Microenvironmental Remodeling for Endometrial Repair and Fertility Restoration

Advanced Science, EarlyView.
A bioactive adhesive hydrogel capable of long‐term uterine retention (>14 days) and local microenvironment modulation is developed by straightforward integration of α‐lipoic acid, L‐arginine, tannic acid, and Lactobacillus johnsonii, which can markedly enhance endometrial repair and restore fertility in a rat model of endometrial injury.
Lifa Chen, Hui Zhou, Yifei Li, Yi Liang, Yifan Zhang, Huiyu Huang, Qiuxian Xie, Youchen Tang, Huixia Ye, Yu Zhang   +9 more
wiley   +1 more source

16S rRNA Sequencing FAFSA files

FAFSA files following 16S rRNA bacterial sequencing in fecal samples of adult zebrafish.
Hemaa Sree Kumar (17841158)
core   +1 more source

Isolates 16S rRNA gene sequence

, 2021
16S rRNA gene sequence of isolates from wastewater treated with constructed wetlands obtained with Sanger ...
Camila A. Knecht (11587978)
core   +1 more source

Photobiomodulation‑Engineered Extracellular Vesicles Enhance Neural Differentiation via UFL1‑Mediated UFMylation in Spinal Cord Injury

Advanced Science, EarlyView.
Photobiomodulated microglia release engineered extracellular vesicles that deliver UFL1 to the injured spinal cord. UFL1 competitively binds p53 with MDM2, inhibiting p53 ubiquitination and stabilizing p53 signaling. This dual mechanism simultaneously promotes anti‐inflammatory microglial polarization and directs neural stem cell differentiation toward
Yunxiao Fang, Zuomeng Wu, Ruocheng Guo, Cancan Wang, Yue Qin, Yixiang Dong, Aoyun Li, Ao Liu, Chongyu Jia, Tianyu Han, Peiwen Song, Lei Chen, Cailiang Shen   +12 more
wiley   +1 more source

High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota [PDF]

, 2008
The human gastrointestinal (GI) tract microbiota plays a pivotal role in our health. For more than a decade a major input for describing the diversity of the GI tract microbiota has been derived from the application of small subunit ribosomal RNA (SSU ...
Vos, W.M., de, de Vos, Willem M., Zoetendal, Erwin G., Rajilic-Stojanovic, M., Zoetendal, E.G., Rajilić-Stojanović, Mirjana   +5 more
core   +1 more source

Overcoming Drug Resistance by Paclitaxel Resistance in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
In the murine triple‐negative breast cancer (TNBC) model, chemotherapy effectively increases tumor neoantigen burden (TNB). Here, the study constructs a liposomal nanovaccine using antigens derived from in vitro chemotherapy‐treated paclitaxel‐resistant TNBC 4T1 cells.
Bo Chen, Yangyi Wang, Tingting Li, Changqing Guo, Zhe Xie, Zhixian Zhang, Xinghua Han, Wei Wang, Jian Wang, Xiaopeng Ma, Yuanzeng Min   +10 more
wiley   +1 more source