Results 11 to 20 of about 107,983 (304)

Estimation of 18S Gene Copy Number in Marine Eukaryotic Plankton Using a Next-Generation Sequencing Approach

open access: yesFrontiers in Marine Science, 2019
The small subunit 18S rRNA (18S) gene is the most commonly used marker for taxonomic identification in eukaryotes. However, protists may harbor substantial variation in their 18S gene copy number, which can lead to a rapid decline in concordance between ...
Weida Gong, Adrian Marchetti
doaj   +3 more sources

Reference Gene Selection for Expression Analyses by qRT-PCR in Dendroctonus valens

open access: yesInsects, 2020
Dendroctonus valens is the main pest of the genus Pinus. To facilitate gene expression analyses, suitable reference genes for adults and mature larvae of D. valens under different temperature conditions were determined.
Chunchun Zheng   +5 more
doaj   +1 more source

Molecular epidemiological study of Trichomonas gallinae focusing on central and southeastern Europe

open access: yesFrontiers in Veterinary Science, 2022
Trichomonas gallinae is a geographically widespread protozoan parasite of birds. In this study, oropharyngeal swab samples were collected in Hungary and Romania from 99 columbiform birds, including 76 feral pigeons (Columba livia domestica: 42 kept for ...
Barbara Tuska-Szalay   +15 more
doaj   +1 more source

Theileria terrestris nov. sp.: A Novel Theileria in Lowland Tapirs (Tapirus terrestris) from Two Different Biomes in Brazil

open access: yesMicroorganisms, 2022
The low-land tapir (Tapirus terrestris) is the largest wild terrestrial mammal found in Brazil. Although T. terrestris has been already reported as a host of hemoparasites, the occurrence and genetic identity of Piroplasmida agents in this species is ...
Anna Claudia Baumel Mongruel   +8 more
doaj   +1 more source

Characterization of the two intra-individual sequence variants in the 18S rRNA gene in the plant parasitic nematode, Rotylenchulus reniformis. [PDF]

open access: yesPLoS ONE, 2013
The 18S rRNA gene is fundamental to cellular and organismal protein synthesis and because of its stable persistence through generations it is also used in phylogenetic analysis among taxa.
Seloame T Nyaku   +5 more
doaj   +1 more source

Molecular differentiation of Thysaniezia (Helictometra) giardi and Moniezia species based on 18s rRNA gene in small ruminants [PDF]

open access: yesIraqi Journal of Veterinary Sciences, 2021
This study was conducted to investigate Anoplocephalidia Cestoda in sheep and goat and evaluate the 18s rRNA to genetically differentiate the genera of this family. Sixty sample tapeworms were collected from small intestines of 30 sheep and 30 goats from
Monyer A. Alfatlawi   +4 more
doaj   +1 more source

Validation of a PNA Clamping Method for Reducing Host DNA Amplification and Increasing Eukaryotic Diversity in Rhizosphere Microbiome Studies

open access: yesPhytobiomes Journal, 2020
Protists and microscopic animals are important but poorly understood determinants of plant health. Plant-associated eukaryotes could be surveyed by high-throughput sequencing of 18S ribosomal RNA (rRNA) genes but the abundance of plant DNA in rhizosphere
Stephen J. Taerum   +3 more
doaj   +1 more source

Parasites and blood-meal hosts of the tsetse fly in Tanzania: a metagenomics study

open access: yesParasites & Vectors, 2022
Background Tsetse flies can transmit various Trypanosoma spp. that cause trypanosomiasis in humans, wild animals, and domestic animals. Amplicon deep sequencing of the 12S ribosomal RNA (rRNA) gene can be used to detect mammalian tsetse hosts, and the ...
Ju Yeong Kim   +4 more
doaj   +1 more source

Free-living protozoa in two unchlorinated drinking water supplies identified by phylogenic analysis of 18S rRNA gene sequences [PDF]

open access: yes, 2009
Free-living protozoan communities in water supplies may include hosts for Legionella pneumophila and other undesired bacteria and also pathogens. This study aimed at identifying free-living protozoa in two unchlorinated groundwater supplies using ...
Valster, R.M.   +4 more
core   +1 more source

Reference genes for quantitative real-time PCR analysis and quantitative expression of P5CS in Agropyron mongolicum under drought stress

open access: yesJournal of Integrative Agriculture, 2016
Reference genes, stably expressing in different tissues and cells, are commonly used as the references in expression analysis. Selecting the optimum reference gene is crucial to the success of experiments.
Qing-song TIAN   +5 more
doaj   +1 more source

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