The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability [PDF]
Myc-induced SUN domain–containing protein (Misu or NSun2) is a nucleolar RNA methyltransferase important for c-Myc–induced proliferation in skin, but the mechanisms by which Misu contributes to cell cycle progression are unknown.
Shobbir Hussain +49 more
core +1 more source
Phylogeny as a proxy for ecology in seagrass amphipods: which traits are most conserved? [PDF]
Increasingly, studies of community assembly and ecosystem function combine trait data and phylogenetic relationships to gain novel insight into the ecological and evolutionary constraints on community dynamics.
Best, Rebecca J, Stachowicz, John J
core +6 more sources
Ecological assessment of phytoplankton community via microscopic method and 18S rRNA gene sequencing in Pearl River Estuary [PDF]
Monitoring phytoplankton community underpins our understanding of water quality and ecological functions. In this study, we approached phytoplankton abundance, community composition, and diversity by both microscopy and 18S rRNA gene sequencing ...
Peng Bo +5 more
doaj +1 more source
Although bats can serve as reservoirs for several viruses and bacteria, there is limited knowledge regarding the diversity of apicomplexan protozoan belonging to the Piroplasmida and Haemosporida orders within this group of mammals.
Victória Valente Califre de Mello +7 more
doaj +1 more source
Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction [PDF]
The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania ...
Barroso, Paola Andrea +11 more
core +1 more source
Validation of suitable internal control genes for expression studies in aging. [PDF]
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA +9 more
core +1 more source
Novel Cryptosporidium genotype in wild Australian mice (Mus domesticus) [PDF]
A total of 250 mouse fecal specimens collected from crop farms in Queensland, Australia, were screened for the presence of Cryptosporidium spp. using PCR.
Boxell, A. +4 more
core +3 more sources
Two Species of Canine Babesia in Australia: Detection and Characterization by PCR [PDF]
The haemoprotozoan Babesia canis has been recognized in Australia for many years, and a second, smaller species has recently been discovered. Amplification and sequencing of a partial region of the 18S small subunit ribosomal RNA (rRNA) gene enabled ...
Irwin, P.J. +3 more
core +2 more sources
A Complete Expression Profile of Matrix-Degrading Metalloproteinases in Dupuytren’s Disease [PDF]
Dupuytren’s disease (DD) is a common fibrotic condition of the palmar fascia, leading to deposition of collagen-rich cords and finger contractions. The metzincin superfamily contains key enzymes in the turnover of collagen and other extracellular matrix ...
Adrian J. Chojnowski +48 more
core +1 more source
Nematode‐specific PCR primers for the 18S small subunit rRNA gene
AbstractA set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are
Floyd, R +3 more
openaire +3 more sources

