Results 21 to 30 of about 64,103 (261)

Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA gene [PDF]

FEMS Microbiology Ecology, 2005
A quantitative PCR (QPCR) assay based on the use of SYBR Green I was developed to assess the abundance of specific groups of picoeukaryotes in marine waters. Six primer sets were designed targeting four different taxonomic levels: domain (Eukaryota), division (Chlorophyta), order (Mamiellales) and genus (Bathycoccus, Micromonas, and Ostreococcus ...
Zhu, F., Massana, R., Not, F., Marie, D., Vaulot, D.   +4 more
openaire   +4 more sources

Prevalence and molecular characterization of trypanosoma spp in domestic geese (anser anser domesticus) from the north-east anatolia region of turkey [PDF]

Kafkas Universitesi Veteriner Fakültesi Dergisi, 2018
Avian Trypanosomiosis is a protozoan disease transmitted by blood-sucking arthropods belonging to the Simuliidae, Culicidae, Ceratopogonidae, Hippoboscidae, and Dermanyssidae. Trypanosoma spp.
Gencay Taşkın TAŞÇI, Zati VATANSEVER, Nilgün PARMAKSIZOĞLU AYDIN, Neslihan ÖLMEZ, Atila AKÇA, Mükremin Özkan ARSLAN, Neriman MOR, Barış SARI   +7 more
doaj   +1 more source

Characterization of the 18S rRNA Gene for Designing Universal Eukaryote Specific Primers

PLoS ONE, 2014
High throughput sequencing technology has great promise for biodiversity studies. However, an underlying assumption is that the primers used in these studies are universal for the prokaryotic or eukaryotic groups of interest. Full primer universality is difficult or impossible to achieve and studies using different primer sets make biodiversity ...
Hadziavdic, Kenan, Lekang, Katrine, Lanzén, Anders, Jonassen, Inge, Thompson, Eric, Troedsson, Christofer   +5 more
openaire   +6 more sources

16S rRNA gene and 18S rRNA gene diversity in microbial mat communities in meltwater ponds on the McMurdo Ice Shelf, Antarctica [PDF]

Polar Biology, 2021
AbstractThe undulating ice of the McMurdo Ice Shelf, Southern Victoria Land, supports one of the largest networks of ice-based, multiyear meltwater pond habitats in Antarctica, where microbial mats are abundant and contribute most of the biomass and biodiversity.
Eleanor E. Jackson, Ian Hawes, Anne D. Jungblut   +2 more
openaire   +1 more source

Preferential suppression of Anopheles gambiae host sequences allows detection of the mosquito eukaryotic microbiome

Scientific Reports, 2017
Anopheles mosquitoes are vectors of the human malaria parasite, Plasmodium falciparum. The vector microbiota is a likely factor influencing parasite transmission.
Eugeni Belda, Boubacar Coulibaly, Abdrahamane Fofana, Abdoul H. Beavogui, Sekou F. Traore, Daryl M. Gohl, Kenneth D. Vernick, Michelle M. Riehle   +7 more
doaj   +1 more source

PCR-based detection of Plasmodium falciparum in saliva using mitochondrial cox3 and varATS primers

Tropical Medicine and Health, 2018
Background Sampling of saliva for diagnosing Plasmodium falciparum infections is a safe, non-invasive alternative to sampling of blood. However, the use of saliva presents a challenge because lower concentrations of parasite DNA are present in saliva ...
Yukie M. Lloyd, Livo F. Esemu, Jovikka Antallan, Bradley Thomas, Samuel Tassi Yunga, Bekindaka Obase, Nana Christine, Rose G. F. Leke, Richard Culleton, Kenji Obadiah Mfuh, Vivek R. Nerurkar, Diane Wallace Taylor   +11 more
doaj   +1 more source

Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing

Microbiome, 2018
Background Targeted amplicon deep sequencing (TADS) of the 16S rRNA gene is commonly used to explore and characterize bacterial microbiomes. Meanwhile, attempts to apply TADS to the detection and characterization of entire parasitic communities have been
Briana R. Flaherty, Eldin Talundzic, Joel Barratt, Kristine J. Kines, Christian Olsen, Meredith Lane, Mili Sheth, Richard S. Bradbury   +7 more
doaj   +1 more source

Estimation of 18S Gene Copy Number in Marine Eukaryotic Plankton Using a Next-Generation Sequencing Approach

Frontiers in Marine Science, 2019
The small subunit 18S rRNA (18S) gene is the most commonly used marker for taxonomic identification in eukaryotes. However, protists may harbor substantial variation in their 18S gene copy number, which can lead to a rapid decline in concordance between ...
Weida Gong, Adrian Marchetti
doaj   +1 more source

Unusual Intraindividual Variation of the Nuclear 18S rRNA Gene is Widespread Within the Acipenseridae [PDF]

Journal of Heredity, 2006
Significant intraindividual variation in the sequence of the 18S rRNA gene is unusual in animal genomes. In a previous study, multiple 18S rRNA gene sequences were observed within individuals of eight species of sturgeon from North America but not in the North American paddlefish, Polyodon spathula, in two species of Polypterus (Polypterus delhezi and ...
Jeannette, Krieger, Anne Kathrin, Hett, Paul A, Fuerst, Vadim J, Birstein, Arne, Ludwig   +4 more
openaire   +2 more sources

Characterization of Hydrocortisone Biometabolites and 18S rRNA Gene in Chlamydomonas reinhardtii Cultures

Molecules, 2008
A unicellular microalga, Chlamydomonas reinhardtii, was isolated from rice paddy-field soil and water samples and used in the biotransformation of hydrocortisone (1). This strain has not been previously tested for steroid bioconversion. Fermentation was carried out in BG-11 medium supplemented with 0.05% substrate at 25ºC for 14 days of incubation. The
Ghasemi, Younes, Rasoul-Amini, Sara, Morowvat, Mohammad Hossein, Raee, Mohammad Javad, Ghoshoon, Mohammad Bagher, Nouri, Fatemeh, Negintaji, Narges, Parvizi, Rezvan, Mosavi-Azam, Seyed Bagher   +8 more
openaire   +3 more sources