Results 31 to 40 of about 64,103 (261)

Tissue-specific validation of reference genes for accurate qRT-PCR analysis during sex differentiation in Amphiprion ocellaris [PDF]

Brazilian Journal of Biology
Quantitative real-time PCR (qRT-PCR) is a widely used method for measuring gene expression, but its reliability depends on using stable reference genes for normalization.
C. Boonphakdee, J. Thamnawasolos, T. Boonphakdee, T. Mataroepayotorn   +3 more
doaj   +1 more source

Nematode‐specific PCR primers for the 18S small subunit rRNA gene

Molecular Ecology Notes, 2005
AbstractA set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are
Floyd, R, Rogers, A, Lambshead, P, Smith, C   +3 more
openaire   +3 more sources

Optimal eukaryotic 18S and universal 16S/18S ribosomal RNA primers and their application in a study of symbiosis.

PLoS ONE, 2014
Eukaryotic 18S ribosomal RNA (rRNA) gene primers that feature a wide coverage are critical in detecting the composition of eukaryotic microscopic organisms in ecosystems.
Yong Wang, Ren Mao Tian, Zhao Ming Gao, Salim Bougouffa, Pei-Yuan Qian   +4 more
doaj   +1 more source

Moleculer Detection of Protozoa Trichodina spp. In Gourami (Osphromenus Gourame Lac.) Larvae with The infecting 18S rRNA Gene Marking in Exs. Residence of Banyumas, Central Java

Biosaintifika: Journal of Biology & Biology Education, 2018
Protozoa species of Trichodina spp. may be found in most hatchery centers in Banyumas, Purbalingga, and Banjarnegara. However, the determination of Trichodina spp. types is still based on its body’s morphological variations, not yet molecular. A research
Rokhmani Rokhmani, Endang Ariyani Setyawati, Daniel Joko Wahyono   +2 more
doaj   +1 more source

A phylogenetic framework for the kingdom Fungi based on 18S rRNA gene sequences

Marine Genomics, 2017
The usage of molecular phylogenetic approaches is critical to advance the understanding of systematics and community processes in the kingdom Fungi. Among the possible phylogenetic markers (or combinations of them), the 18S rRNA gene appears currently as the most prominent candidate due to its large availability in public databases and informative ...
Pablo Yarza, Pelin Yilmaz, Katrin Panzer, Frank Oliver Glöckner, Marlis Reich   +4 more
openaire   +3 more sources

The human gut microbiome across the life course

FEBS Letters, EarlyView.
Despite significant individual variation and continuous change throughout life, the human gut microbiome follows some life stage‐specific trends. This article provides a brief overview of how gut microbiome composition shifts across different phases of life. Created in BioRender. Özkurt, E. (2026) https://BioRender.com/8q4nrnc.
Alise J. Ponsero, Basak Bahcivanci, Antonietta Hayhoe, Animesh Acharjee, Ezgi Özkurt   +4 more
wiley   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis [PDF]

BMC Ophthalmology, 2008
Abstract Background The sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis.
Embong Zunaina, Wan Hazabbah Wan Hitam, Chan Yean Yean, Nur Haslindawaty Abdul Rashid, Balqis Kamarudin, Siti Khaironi Zainal Abidin, Sabariah Osman, Zainuddin Zafarina, Manickam Ravichandran   +8 more
openaire   +3 more sources

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Effects of gene therapy on muscle 18S rRNA expression in mouse model of ALS [PDF]

BMC Research Notes, 2010
The efficiency of gene therapy experiments is frequently evaluated by measuring the impact of the treatment on the expression of genes of interest by quantitative real time PCR (qRT-PCR) and by normalizing these values to those of housekeeping (HK) genes constitutively expressed throughout the experiment.
Toivonen Janne M, Calvo Ana C, Oliván Sara, Manzano Raquel, Moreno-Igoa María, Osta Rosario   +5 more
openaire   +3 more sources