Results 41 to 50 of about 4,298,356 (197)

Highly sensitive quantification of key regulatory oxysterols in biological samples by LC-ESI-MS/MSs⃞

Journal of Lipid Research, 2009
We describe a highly sensitive and specific method for the quantification of key regulatory oxysterols in biological samples. This method is based upon a stable isotope dilution technique by liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
Akira Honda, Kouwa Yamashita, Takashi Hara, Tadashi Ikegami, Teruo Miyazaki, Mutsumi Shirai, Guorong Xu, Mitsuteru Numazawa, Yasushi Matsuzaki   +8 more
doaj   +1 more source

24S-hydroxycholesterol and 25-hydroxycholesterol differentially impact hippocampal neuronal survival following oxygen-glucose deprivation. [PDF]

PLoS ONE, 2017
N-methyl-D-aspartate receptors (NMDARs), a major subtype of glutamate receptor mediating excitatory transmission throughout the CNS, participate in ischemia-induced neuronal death.
Min-Yu Sun, Amanda Taylor, Charles F Zorumski, Steven Mennerick   +3 more
doaj   +1 more source

Regulation of endoplasmic reticulum cholesterol by plasma membrane cholesterol

Journal of Lipid Research, 1999
The abundance of cell cholesterol is governed by multiple regulatory proteins in the endoplasmic reticulum (ER) which, in turn, are under the control of the cholesterol in that organelle.
Yvonne Lange, Jin Ye, Mike Rigney, Theodore L. Steck   +3 more
doaj   +1 more source

DataSheet1_Miconazole Suppresses 27-Hydroxycholesterol-induced Inflammation by Regulating Activation of Monocytic Cells to a Proinflammatory Phenotype.docx

, 2021
Miconazole is effective in treating inflammatory skin conditions and has well-established antifungal effects. To elucidate the underlying mechanisms mediating its additional beneficial effects, we assessed whether miconazole influences the inflammation ...
Seong-Kug Eo (3850609), Koanhoi Kim (625698), Yonghae Son (3850612), Dongjun Lee (256900), Bo-Young Kim (516332), Hyok-rae Cho (3850606)   +5 more
core   +1 more source

Spermine synthesis inhibitor blocks 25-hydroxycholesterol-induced- apoptosis via SREBP2 upregulation in DLD-1 cell spheroids

Biochemistry and Biophysics Reports, 2020
The oxysterol 25-hydroxycholesterol (25-HC) has diverse physiological activities, including the ability to inhibit anchorage-independent growth of colorectal cancer cells. Here, we found that a polyamine synthesis inhibitor, DFMO, prevented 25-HC-induced
Miku Kakimoto, Hideya Yamamoto, Arowu R. Tanaka   +2 more
doaj   +1 more source

NCOA4 Accelerates Abdominal Aortic Aneurysm Formation by Regulating Macrophage Activation and Ferroptosis via STAT1–CH25H Axis

Advanced Science, EarlyView.
Under pathological stimulation, NCOA4 in macrophages interacts with STAT1 to promote CH25H transcription. The subsequent increase in 25‐HC production activates macrophages, triggers inflammatory responses and ferroptosis, and accelerates the progression of AAA.
Zhinan Wu, Yuxuan Qian, Wenyi Xu, Fei Liao, Xueliang Liu, Xiao Kuang, Guqing Luo, Xinjie He, Lixian Jiang, Song Ding   +9 more
wiley   +1 more source

Effect of 25-hydroxycholesterol, 26-hydroxycholesterol and its analogue, 26-aminocholesterol, on protein content, protein synthesis and LDH leakage in mouse epidermal cells

, 1995
The cytotoxicity of 25-hydroxycholesterol, 26-hydroxycholesterol and its analogue, 26-aminocholesterol was investigated in murine epidermal cell line, HEL-30.
R. Paoletti, C.L. Galli, F. Ghilardi, M. Guizzetti, M. Marinovich   +4 more
core   +1 more source

Ca2+ channel blockers verapamil and nifedipine inhibit apoptosis induced by 25-hydroxycholesterol in human aortic smooth muscle cells

Journal of Lipid Research, 1997
We have characterized the death of human aortic smooth muscle cells induced by 25-hydroxycholesterol, an oxidation product of cholesterol. Chromatin condensation characteristic of apoptosis was observed by enzymatic (TUNEL) staining of chromatin, and by ...
M P Ares, M I Pörn-Ares, J Thyberg, L Juntti-Berggren, P O Berggren, U Diczfalusy, B Kallin, I Björkhem, S Orrenius, J Nilsson   +9 more
doaj   +1 more source

Aggregation of 25-hydroxycholesterol in a complex biomembrane. Differences with cholesterol

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2020
25-Hydroxycholesterol (25HC), one of the most important oxysterol molecules, can be used by cells to fight bacterial and viral infections but the mechanism that defines its biological effects are unknown. Using molecular dynamics, we have aimed to describe the orientation and location of 25HC in the membrane as well as the interactions it might have ...
Galiano, Vicente, Villalaín, José
openaire   +3 more sources

Binding of 25-hydroxycholesterol and cholesterol to different cytoplasmic proteins [PDF]

Proceedings of the National Academy of Sciences, 1977
Studies were carried out to determine whether or not oxygenated derivatives of cholesterol (e.g., 25-hydroxycholesterol) that specifically suppress the activity of 3-hydroxy-3-methylglutaryl-CoA reductase [mevalonate:NADP + oxidoreductase (CoA-acylating), EC 1.1.1.34], bind to a soluble ...
Kandutsch, A A, Chen, H W, Shown, E P
openaire   +2 more sources