Results 21 to 30 of about 916,340 (306)

3-Dimensional mesothelioma spheroids provide closer to natural pathophysiological tumor microenvironment for drug response studies

Frontiers in Oncology, 2022
Traditional studies using cancer cell lines are often performed on a two-dimensional (2D) cell culture model with a low success rate of translating to Phase I or Phase II clinical studies. In comparison, with the advent of developments three-dimensional (
Huaikai Shi, Emma M. Rath, Ruby C. Y. Lin, Ruby C. Y. Lin, Ruby C. Y. Lin, Kadir Harun Sarun, Candice Julie Clarke, Brian C. McCaughan, Brian C. McCaughan, Helen Ke, Helen Ke, Anthony Linton, Anthony Linton, Anthony Linton, Kenneth Lee, Kenneth Lee, Sonja Klebe, Sonja Klebe, Joanneke Maitz, Joanneke Maitz, Joanneke Maitz, Kedong Song, Yiwei Wang, Yiwei Wang, Steven Kao, Steven Kao, Yuen Yee Cheng   +26 more
doaj   +1 more source

Quantitative volumetric Raman imaging of three dimensional cell cultures [PDF]

, 2017
The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions.
Bergholt, MS, Kallepitis, C, Leonardo, V, Maynard, SA, Mazo, MM, Skaalure, SC, Stevens, MM   +6 more
core   +1 more source

Magnetic Stiffening in 3D Cell Culture Matrices [PDF]

Nano Letters, 2021
The mechanical environment of a cell is not constant. This dynamic behavior is exceedingly difficult to capture in (synthetic) in vitro matrices. This paper describes a novel, highly adaptive hybrid hydrogel composed of magnetically sensitive magnetite nanorods and a stress-responsive synthetic matrix. Nanorod rearrangement after application of (small)
Wen Chen, Ying Zhang, Jyoti Kumari, Hans Engelkamp, Paul H. J. Kouwer   +4 more
openaire   +4 more sources

Muscle injury-induced hypoxia alters the proliferation and differentiation potentials of muscle resident stromal cells

Skeletal Muscle, 2019
Background Trauma-induced heterotopic ossification (HO) is a complication that develops under three conditions: the presence of an osteogenic progenitor cell, an inducing factor, and a permissive environment. We previously showed that a mouse multipotent
Geneviève Drouin, Vanessa Couture, Marc-Antoine Lauzon, Frédéric Balg, Nathalie Faucheux, Guillaume Grenier   +5 more
doaj   +1 more source

Current Advances in 3D Dynamic Cell Culture Systems

Gels, 2022
The traditional two-dimensional (2D) cell culture methods have a long history of mimicking in vivo cell growth. However, these methods cannot fully represent physiological conditions, which lack two major indexes of the in vivo environment; one is a ...
Xin Huang, Zhengxiang Huang, Weidong Gao, Wendong Gao, Ruiying He, Yulin Li, Ross Crawford, Yinghong Zhou, Lan Xiao, Yin Xiao   +9 more
doaj   +1 more source

Dorsal and ventral stimuli in cell–material interactions: effect on cell morphology [PDF]

, 2012
Cells behave differently between bidimensional (2D) and tridimensional (3D) environments. While most of the in vitro cultures are 2D, most of the in vivo extracellular matrices are 3D, which encourages the development of more relevant culture conditions,
Curtis ASG, Dunn JC, Hlatky L, Hlatky L, José Ballester-Beltrán, Lewis WH, Manuel Salmerón-Sánchez, Myriam Lebourg, Patricia Rico, Ryan CM   +9 more
core   +2 more sources

Mesenchymal stem cells lose the senescent phenotype under 3D cultivation

Stem Cell Research & Therapy, 2023
Background Three-dimensional (3D) cell culture is widely used in various fields of cell biology. In comparison to conventional two-dimensional (2D) cell culture, 3D cell culture facilitates a more accurate replication of the in vivo microenvironment ...
O. Krasnova, A. Kovaleva, A. Saveleva, K. Kulakova, O. Bystrova, M. Martynova, A. Domnina, J. Sopova, I. Neganova   +8 more
doaj   +1 more source

Adult Multipotent Cardiac Progenitor-Derived Spheroids: A Reproducible Model of In Vitro Cardiomyocyte Commitment and Specification

Cells, 2023
Background: Three-dimensional cell culture systems hold great promise for bridging the gap between in vitro cell-based model systems and small animal models to study tissue biology and disease.
Mariangela Scalise, Fabiola Marino, Luca Salerno, Nunzia Amato, Claudia Quercia, Chiara Siracusa, Andrea Filardo, Antonio Chiefalo, Loredana Pagano, Giuseppe Misdea, Nadia Salerno, Antonella De Angelis, Konrad Urbanek, Giuseppe Viglietto, Daniele Torella, Eleonora Cianflone   +15 more
doaj   +1 more source

Stromal upregulation of lateral epithelial adhesions: gene expression analysis of signalling pathways in prostate epithelium [PDF]

, 2011
Background: Stromal signalling increases the lateral cell adhesions of prostate epithelial cells grown in 3D culture. The aim of this study was to use microarray analysis to identify significant epithelial signalling pathways and genes in this process ...
Chambers, Karen F., Pearson, Joanna F., Pellacani, Davide, Aziz, Naveed, Gužvić, Miodrag, Klein, Christoph A., Lang, Shona H.   +6 more
core   +4 more sources

3D Cardiac Cell Culture: A Critical Review of Current Technologies and Applications

Frontiers in Cardiovascular Medicine, 2019
Three-dimensional (3D) cell culture is often mentioned in the context of regenerative medicine, for example, for the replacement of ischemic myocardium with tissue-engineered muscle constructs.
Christian Zuppinger
doaj   +1 more source