Results 71 to 80 of about 4,889,916 (279)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

UV-induced reorganization of 3D genome mediates DNA damage response

Nature Communications
While it is well-established that UV radiation threatens genomic integrity, the precise mechanisms by which cells orchestrate DNA damage response and repair within the context of 3D genome architecture remain unclear.
Veysel Oğulcan Kaya, Ogün Adebali
doaj   +1 more source

Cognitive and Neuroimaging Divergence Between Juvenile and Adult FUS Amyotrophic Lateral Sclerosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by progressive motor neuron degeneration. Fused in sarcoma (FUS)‐associated juvenile ALS (jALS) represents a distinct and aggressive subgroup with rapid deterioration and poor prognosis.
Alexandra V. Jürs, Marcel Naumann, Annaliis Lehto, Hanna Schön, Jens Kurth, Johannes Prudlo, Andreas Hermann, Elisabeth Kasper   +7 more
wiley   +1 more source

Interphase chromosomes of the Aedes aegypti mosquito are liquid crystalline and can sense mechanical cues

Nature Communications, 2023
Within the nucleus, the genome of eukaryotes folds into partially organized three-dimensional structures specific to each organism. Here the authors perform physical simulations to study the genome architecture of Aedes aegypti, which reveal an ensemble ...
Vinícius G. Contessoto, Olga Dudchenko, Erez Lieberman Aiden, Peter G. Wolynes, José N. Onuchic, Michele Di Pierro   +5 more
doaj   +1 more source

3D genome of multiple myeloma reveals spatial genome disorganization associated with copy number variations

, 2017
The Hi-C method is widely used to study the functional roles of the three-dimensional (3D) architecture of genomes. Here, we integrate Hi-C, whole-genome sequencing (WGS) and RNA-seq to study the 3D genome architecture of multiple myeloma (MM) and how it
Yifang Liu, Ruifeng Li, Ping Zhu, Tingting Li, Lumeng Jia, Yuezhou Yu, Cheng Li, Pengze Wu, Daiwei Tang, Qing Chen   +9 more
core   +1 more source

Self‐Healing Hydrogel‐Enabled Modular Assembly of Bilayered Skin Construct for Hair Follicle Regeneration

Advanced Healthcare Materials, EarlyView.
Self‐healing assembly of two cell‐laden hydrogel layers creates a biomimetic skin construct that restores both dermal and epidermal functions, resulting in accelerated wound healing and enhanced hair follicle regeneration in a full‐thickness skin wound model. ABSTRACT Alopecia remains a pervasive clinical challenge, largely owing to the limited ability
JaeWook Park, Su Yong Kim, MinSu Jeong, Beom Joon Kim, Shin Hyuk Kang, Won‐Gun Koh   +5 more
wiley   +1 more source

Three-dimensional genome architecture connects chromatin structure and function in a major wheat pathogen

BMC Biology
Background Genome spatial organization plays a fundamental role in biological function across all domains of life. While the principles of nuclear architecture have been well-characterized in animals and plants, their functional relevance in filamentous ...
Ivona Glavincheska, Cecile Lorrain
doaj   +1 more source

3D HOP

, 2020
" 3DHOP (3D Heritage Online Presenter) is an open-source software package for the creation of interactive Web presentations of high-resolution 3D models, oriented to the Cultural Heritage field....
Consortium 3D SHS Team
core  

Atom‐Efficient Cerium‐Based Molecular Cages as Biocompatible Reactive Oxygen Scavengers

Advanced Healthcare Materials, EarlyView.
Discrete mixed‐valent cerium molecular cages organize accessible redox centers within confined coordination environments and scavenge superoxide, hydrogen peroxide, and hydroxyl radicals. Encapsulation of the lead cage in an ROS‐responsive DTP nanoplatform enables lesion‐associated delivery, attenuates macrophage oxidative stress and inflammation, and ...
Chao Li, Chunbai Xiang, Ye Xu, Yu Liu, Keze Hong, Fanchu Zeng, Gelin Xu, Zhao‐Xi Wang, Jonathan L. Sessler, Ping Gong   +9 more
wiley   +1 more source

Structural variation drives enhancer hijacking via 3D genome disruption in ccRCC

npj Digital Medicine
Clear cell renal cell carcinoma (ccRCC) is an aggressive malignancy associated with single-nucleotide variants in VHL, PBRM1, and SETD2. However, the role of structural variation (SV)—which have broader genomic impacts—and 3D genome architecture in ccRCC
Yu Dong, Wenjiao Xia, Zitong Yang, Liangliang Ren, Hongru Wang, Yiyang Zhou, Qinchen Li, Zhi Chen, Zhinan Xia, Yichun Zheng, Feifan Wang, Ning He, Bing Cheng, Dongmei Ma, Wei Shao, Wei Guo, Shuwen Wang, Ziqiao Liu, Junxiao Shen, Yiming Qi, Xuke Gong, Juan Jin, Bo Xie, Guixin Zhu, Cheng Zhang   +24 more
doaj   +1 more source