Results 71 to 80 of about 44,474 (235)

A cost‐effective DNA extraction protocol for long‐read sequencing in non‐model plants

Applications in Plant Sciences, EarlyView.
Abstract Premise Third‐generation sequencing has revolutionized genomics, enabling in‐depth analysis of genome sequence, structure, and epigenetic features. Yet, extracting high‐quality DNA for long‐read sequencing remains a bottleneck—particularly in non‐model plants, such as mature trees growing in natural environments, which often contain abundant ...
Sofia Gaischuk, Cintia F. Marchetti, Ezequiel Petrillo, Fernando A. Rabanal, Detlef Weigel, Nicolás Bellora, M. Verónica Arana   +6 more
wiley   +1 more source

Insights into the role of DNA methylation in diatoms by genome-wide profiling in Phaeodactylum tricornutum [PDF]

, 2013
DNA cytosine methylation is a widely conserved epigenetic mark in eukaryotes that appears to have critical roles in the regulation of genome structure and transcription.
Ahmed, Ikhlak, Allen, Andrew, Bhavsar, Jaysheel, Bondurant, Sandra Splinter, Bowler, Chris, Creasy, Todd, Crom, Stéphane Le, Farrant, Gregory K., Lin, Xin, Maumus, Florian, O'Brien, Kimberly, Olson, Sue A., Rabinowicz, Pablo D., Rayko, Edda, Rivarola, Maximo Lisandro, Sengamalay, Naomi A., Sgro, Jean-Yves, Smith, Andrew D., Sussman, Michael R., Tallon, Luke J., Tirichine, Leïla, Veluchamy, Alaguraj   +21 more
core   +3 more sources

5‐Methylcytosine Analysis of miRNAs in Minimal Change Disease

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Minimal change disease (MCD) is a glomerular disorder, which is the most common cause of nephrotic syndrome in children. Additionally, the prevalence of MCD in adults has been increasing in recent years. During protein synthesis, noncoding RNAs can be regulated through a variety of modifications, which helps preserve biological diversity and ...
Huiyi Zeng, Dandan Li, Yunyi Li, Chen Yun, Xinzhou Zhang, Wenyu Gong, Mengyun Xiao, Haitao Li, Zhipeng Zeng, Ruqi Tan, Yishen Yang, Shaodong Luan, Yong Dai, Lianghong Yin, Donge Tang   +14 more
wiley   +1 more source

Epigenetic Regulation in the Pathogenesis of Renal Inflammation: Insights and Therapeutic Potentials

iNew Medicine, EarlyView.
ABSTRACT Renal inflammation is a common pathological process in various kidney diseases, often initiated by factors such as toxins, ischemia, or autoimmune reactions. This inflammatory response can result in structural damage and a rapid decline in renal function.
Yu‐Hang Dong, Xiao‐Yu Shen, Han‐Cheng Jin, Xiang‐Yu Li, Hao He, Xiao‐Ming Meng   +5 more
wiley   +1 more source

Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. [PDF]

PLoS ONE, 2018
5-methylcytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization ...
Aditi Singh, Adrienne Vancura, Rafal K Woycicki, Daniel J Hogan, Alan G Hendrick, Mariusz Nowacki   +5 more
doaj   +1 more source

5-Hydroxymethylcytosine Is Not Present in Appreciable Quantities in Arabidopsis DNA [PDF]

, 2014
5-Hydroxymethylcytosine (5-hmC) is an intermediate in active demethylation in metazoans, as well as a potentially stable epigenetic mark. Previous reports investigating 5-hydroxymethylcytosine in plants have reached conflicting conclusions.
Clish, Clary, Erdmann, Robert Michael, Gehring, Mary, Souza, Amanda L.   +3 more
core   +1 more source

Direct Sensing of 5‐Methylcytosine by Polymerase Chain Reaction [PDF]

Angewandte Chemie International Edition, 2014
AbstractThe epigenetic control of genes by the methylation of cytosine resulting in 5‐methylcytosine (5mC) has fundamental implications for human development and disease. Analysis of alterations in DNA methylation patterns is an emerging tool for cancer diagnostics and prognostics.
Aschenbrenner, Joos, Drum, Matthias, Topal, Hüsnü, Wieland, Markus, Marx, Andreas   +4 more
openaire   +3 more sources

Noncanonical Nucleotides in the Genome Around the Maternal‐Zygotic Transition

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
In this paper, Kazzazi et al. provide a comprehensive review of the dynamics of nonconventional nucleotides in the genome during early developmental stages, hypothesizing a potential role for these nucleotides in the activation of the zygotic genome. ABSTRACT From the very moment of fertilization and throughout development, the cells of animal embryos ...
Latifa Kazzazy, Dávid Mező, Kinga K. Nagy, Viktória Perey‐Simon, Judit Tóth, Angéla Békési, Beáta Vértessy, Máté Varga   +7 more
wiley   +1 more source

Regional differences in mitochondrial DNA methylation in human post-mortem brain tissue

Clinical Epigenetics, 2017
Background DNA methylation is an important epigenetic mechanism involved in gene regulation, with alterations in DNA methylation in the nuclear genome being linked to numerous complex diseases.
Matthew Devall, Rebecca G. Smith, Aaron Jeffries, Eilis Hannon, Matthew N. Davies, Leonard Schalkwyk, Jonathan Mill, Michael Weedon, Katie Lunnon   +8 more
doaj   +1 more source

African Trypanosomes Contain 5-Methylcytosine in Nuclear DNA [PDF]

Eukaryotic Cell, 2008
ABSTRACT It is currently unclear if there are modified DNA bases in Trypanosoma brucei other than J-base. We identify herein a cytosine-5 DNA methyltransferase gene and report the presence and location of 5-methylcytosine in genomic DNA.
Kevin T, Militello, Ping, Wang, Sangeeta K, Jayakar, Rebecca L, Pietrasik, Christopher D, Dupont, Kristi, Dodd, Anthony M, King, Paul R, Valenti   +7 more
openaire   +2 more sources