Results 81 to 90 of about 401,347 (255)

Electron Cryomicroscopy Structure of a Membrane-anchored Mitochondrial AAA Protease [PDF]

Journal of Biological Chemistry, 2011
FtsH-related AAA proteases are conserved membrane-anchored, ATP-dependent molecular machines, which mediate the processing and turnover of soluble and membrane-embedded proteins in eubacteria, mitochondria, and chloroplasts. Homo- and hetero-oligomeric proteolytic complexes exist, which are composed of homologous subunits harboring an ATPase domain of ...
Lee, S., Augustin, S., Tatsuta, T., Gerdes, F., Langer, T., Tsai, F.   +5 more
openaire   +3 more sources

Multistep substrate binding and engagement by the AAA+ ClpXP protease [PDF]

Proceedings of the National Academy of Sciences, 2020
Significance AAA+ proteases play key regulatory and quality control roles in all domains of life. These destructive enzymes recognize damaged, unneeded, or regulatory proteins via specific degrons and unfold them prior to processive degradation. Here, we show that Escherichia coli
Reuben A. Saunders, Benjamin M. Stinson, Tania A. Baker, Robert T. Sauer   +3 more
openaire   +2 more sources

Insertion Defects of Mitochondrially Encoded Proteins Burden the Mitochondrial Quality Control System

Cells, 2018
The mitochondrial proteome contains proteins from two different genetic systems. Proteins are either synthesized in the cytosol and imported into the different compartments of the organelle or directly produced in the mitochondrial matrix.
Braulio Vargas Möller-Hergt, Andreas Carlström, Tamara Suhm, Martin Ott   +3 more
doaj   +1 more source

ALIX binds a YPX(3)L motif of the GPCR PAR1 and mediates ubiquitin-independent ESCRT-III/MVB sorting. [PDF]

, 2012
The sorting of signaling receptors to lysosomes is an essential regulatory process in mammalian cells. During degradation, receptors are modified with ubiquitin and sorted by endosomal sorting complex required for transport (ESCRT)-0, -I, -II, and -III ...
Chen, Buxin, Dores, Michael R, Lin, Huilan, Meerloo, Timo, Montagne, William A, Paing, May M, Soh, Unice JK, Trejo, JoAnn   +7 more
core   +3 more sources

Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli

Bio-Protocol, 2020
Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the protein
Ryan Cupo, James Shorter
doaj   +1 more source

Fluorescence Methods Applied to the Description of Urea-Dependent YME1L Protease Unfolding

Biomolecules, 2020
ATP-dependent proteases are ubiquitous across all kingdoms of life and are critical to the maintenance of intracellular protein quality control. The enzymatic function of these enzymes requires structural stability under conditions that may drive ...
Sydney Moore, Alyssa Pickens, Jessica L. Rodriguez, Justin D. Marsee, Justin M. Miller   +4 more
doaj   +1 more source

Regulation of the Essential Transmembrane AAA+ Protease FtsH by HflK/C Oligomeric Assembly

Structural Dynamics
Membrane-anchored AAA+ proteases, such as FtsH, degrade membrane-bound and soluble substrates to maintain protein homeostasis and regulate cellular functions across a diverse array of organisms and organelles, including eubacteria, chloroplasts ...
Alireza Ghanbarpour, Jia Jia Zhang, Bertina Telusma, B. Powell, Isabella Bolstad, T. Baker, Sandro Keller, Robert T. Sauer, Joseph H. Davis   +8 more
semanticscholar   +1 more source

Toxic Activation of an AAA+ Protease by the Antibacterial Drug Cyclomarin A.

Cell Chemical Biology, 2019
ATP-driven bacterial AAA+ proteases have been recognized as drug targets. They possess an AAA+ protein (e.g., ClpC), which threads substrate proteins into an associated peptidase (e.g., ClpP).
M. Maurer, D. Linder, Kamila B. Franke, Jasmin Jäger, G. Taylor, Felix Gloge, Sebastian Gremer, L. Le Breton, M. Mayer, E. Weber-Ban, M. Carroni, B. Bukau, A. Mogk   +12 more
semanticscholar   +1 more source

Rapid enzymatic test for phenotypic HIV protease drug resistance [PDF]

, 2003
A phenotypic resistance test based on recombinant expression of the active HIV protease in E. coli from patient blood samples was developed. The protease is purified in a rapid onestep procedure as active enzyme and tested for inhibition by five selected
Assfalg-Machleidt, Irmgard, Helm, K. von der, Hoffmann, D., Koszinowski, U., Machleidt, Werner, Nitschko, H.   +5 more
core   +1 more source

Mechanical Protein Unfolding and Translocation by AAA+ Proteases [PDF]

Biophysical Journal, 2014
In all cells, protein degradation regulates key processes and maintains protein quality control. The AAA+ protease ClpXP is one of the best-studied examples of the ATP-dependent machinery responsible for intracellular proteolysis. A ring-shaped hexamer of the ClpX ATPase first recognizes and denatures folded protein substrate, then translocates the ...
Olivares, Adrian O., Cordvoa, Juan Carlos, Calmat, Stephane, Lang, Matthew J., Sauer, Robert T., Baker, Tania A.   +5 more
openaire   +1 more source