Results 61 to 70 of about 63,350 (232)

Mechanical Protein Unfolding and Translocation by AAA+ Proteases [PDF]

Biophysical Journal, 2014
In all cells, protein degradation regulates key processes and maintains protein quality control. The AAA+ protease ClpXP is one of the best-studied examples of the ATP-dependent machinery responsible for intracellular proteolysis. A ring-shaped hexamer of the ClpX ATPase first recognizes and denatures folded protein substrate, then translocates the ...
Olivares, Adrian O., Cordvoa, Juan Carlos, Calmat, Stephane, Lang, Matthew J., Sauer, Robert T., Baker, Tania A.   +5 more
openaire   +1 more source

Molecular Characterization of the ClpC AAA+ ATPase in the Biology of Chlamydia trachomatis

mBio, 2023
Bacterial AAA+ unfoldases are crucial for bacterial physiology by recognizing specific substrates and, typically, unfolding them for degradation by a proteolytic component. The caseinolytic protease (Clp) system is one example where a hexameric unfoldase
Stefan Pan, Aaron A. Jensen, Nicholas A. Wood, Beate Henrichfreise, Heike Brötz-Oesterhelt, Derek J. Fisher, Peter Sass, Scot P. Ouellette   +7 more
doaj   +1 more source

Oma1 Links Mitochondrial Protein Quality Control and TOR Signaling To Modulate Physiological Plasticity and Cellular Stress Responses [PDF]

, 2016
ACKNOWLEDGMENTS We thank Dennis Winge (University of Utah) and the members of the Khalimonchuk laboratory for critical comments. We also thank Christoph Schuller (University of Natural Resources, Austria) and Paul Herman (Ohio State University) for ...
Barrientos, Antoni, Bohovych, Iryna, Brown, Alistair J.P., Christianson, Sara, Fangman, Teresa, Kastora, Stavroula, Khalimonchuk, Oleh, Kim, Heejeong, Lee, Jaekwon, Topil, Danelle, Zhou, You J.   +10 more
core   +2 more sources

Regulation of mitochondrial plasticity by the i-AAA protease YME1L [PDF]

Biological Chemistry, 2020
Abstract Mitochondria are multifaceted metabolic organelles and adapt dynamically to various developmental transitions and environmental challenges. The metabolic flexibility of mitochondria is provided by alterations in the mitochondrial proteome and is tightly coupled to changes in the shape of mitochondria. Mitochondrial proteases are
Yohsuke Ohba, Thomas MacVicar, Thomas Langer   +2 more
openaire   +3 more sources

Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations

eLife, 2020
Cells have evolved specialized protein disaggregases to reverse toxic protein aggregation and restore protein functionality. In nonmetazoan eukaryotes, the AAA+ disaggregase Hsp78 resolubilizes and reactivates proteins in mitochondria. Curiously, metazoa
Ryan R Cupo, James Shorter
doaj   +1 more source

Protein Processing in Plant Mitochondria Compared to Yeast and Mammals

Frontiers in Plant Science, 2022
Limited proteolysis, called protein processing, is an essential post-translational mechanism that controls protein localization, activity, and in consequence, function. This process is prevalent for mitochondrial proteins, mainly synthesized as precursor
Malgorzata Heidorn-Czarna, Agata Maziak, Hanna Janska   +2 more
doaj   +1 more source

Funktion der m-AAA-Protease bei der Regulation mitochondrialer Translation [PDF]

, 2005
Proteolytische Prozesse spielen bei der Qualitätskontrolle mitochondrialer Proteine und der Biogenese von Mitochondrien eine wichtige Rolle. So führt eine Inaktivierung der ATP-abhängigen m-AAA-Protease in verschiedenen Organismen zu einer gestörten ...
Nolden, Mark
core  

Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK–TssFG subcomplex [PDF]

, 2014
Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However, the mechanism of the T6SS is incompletely understood. In the present study, we investigated a conserved component of
Alan R. Prescott, Aschtgen, Aschtgen, Aschtgen, Aschtgen, Ballister, Basler, Basler, Bonemann, Bonemann, Boyer, Burtnick, Casabona, Casabona, Cascales, Coulthurst, Dong, English, Felisberto-Rodrigues, Francesca R. Cianfanelli, Fritsch, Gerlach, Grant English, Holland, Hood, Jack, Kaniga, Kapitein, Kapitein, Laue, Leiman, Leiman, Lossi, Ma, Ma, Ma, MacIntyre, Mougous, Murdoch, Olwyn Byron, Pell, Potvin, Pukatzki, Rao, Rickman, Russell, Russell, Sambrook, Sarah J. Coulthurst, Schell, Schuck, Schwarz, Shneider, Silverman, Srikannathasan, Suarez, Vistica, Whitney, Zheng, Zheng, Zoued   +60 more
core   +3 more sources

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Proteomics of Cytochrome c Oxidase-Negative versus -Positive Muscle Fiber Sections in Mitochondrial Myopathy [PDF]

, 2019
The mosaic distribution of cytochrome c oxidase(+) (COX+) and COX - muscle fibers in mitochondrial disorders allows the sampling of fibers with compensated and decompensated mitochondrial function from the same individual.
Doll, S., Geyer, P., Klopstock, T., Mann, M., Murgia, M., Tan, J.   +5 more
core   +3 more sources