Results 11 to 20 of about 214,689 (295)

Auxin‐mediated induction of GAL promoters by conditional degradation of Mig1p improves sesquiterpene production in Saccharomyces cerevisiae with engineered acetyl‐CoA synthesis

Microbial Biotechnology, 2021
Summary The yeast Saccharomyces cerevisiae uses the pyruvate dehydrogenase‐bypass for acetyl‐CoA biosynthesis. This relatively inefficient pathway limits production potential for acetyl‐CoA‐derived biochemical due to carbon loss and the cost of two high ...
Irfan Farabi Hayat, Manuel Plan, Birgitta E. Ebert, Geoff Dumsday, Claudia E. Vickers, Bingyin Peng   +5 more
doaj   +2 more sources

Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds

ACS Synthetic Biology, 2023
Acetyl-CoA is an essential central metabolite in living organisms and a key precursor for various value-added products as well. However, the intracellular availability of acetyl-CoA limits the efficient production of these target products due to complex and strict regulation. Here, we proposed a new acetyl-CoA pathway, relying on two enzymes, threonine
Mengzhen Nie, Jingyu Wang, Kechun Zhang
openaire   +3 more sources

Chemical Genetics of Acetyl-CoA Carboxylases [PDF]

Molecules, 2013
Chemical genetic studies on acetyl-CoA carboxylases (ACCs), rate-limiting enzymes in long chain fatty acid biosynthesis, have greatly advanced the understanding of their biochemistry and molecular biology and promoted the use of ACCs as targets for herbicides in agriculture and for development of drugs for diabetes, obesity and cancers.
Xuyu Zu, Jing Zhong, Dixian Luo, Jingjing Tan, Qinghai Zhang, Ying Wu, Jianghua Liu, Renxian Cao, Gebo Wen, Deliang Cao   +9 more
openaire   +5 more sources

Multi-subunit acetyl-CoA carboxylases

Progress in Lipid Research, 2002
Acetyl-CoA carboxylase (ACC) catalyses the first committed step of fatty acid synthesis, the carboxylation of acetyl-CoA to malonyl-CoA. Two physically distinct types of enzymes are found in nature. Bacterial and most plant chloroplasts contain a multi-subunit ACC (MS-ACC) enzyme that is readily dissociated into its component proteins.
John E, Cronan, Grover L, Waldrop
openaire   +3 more sources

Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases by alternative pathways for cytosolic acetyl-CoA synthesis

Metabolic Engineering, 2014
Cytosolic acetyl-coenzyme A is a precursor for many biotechnologically relevant compounds produced by Saccharomyces cerevisiae. In this yeast, cytosolic acetyl-CoA synthesis and growth strictly depend on expression of either the Acs1 or Acs2 isoenzyme of acetyl-CoA synthetase (ACS).
Kozak, B.U. (author), Van Rossum, H.M. (author), Benjamin, K.R. (author), Wu, L. (author), Daran, J.G. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)   +6 more
openaire   +5 more sources

Interaction of acetyl‐CoA fragments with rat liver acetyl‐CoA carboxylase [PDF]

European Journal of Biochemistry, 1990
The interaction of acetyl‐CoA fragments with rat liver acetyl‐CoA carboxylase has been studied. Dephosphorylated acetyl‐CoA did not actually differ from acetyl‐CoA in its substrate properties. Non‐nucleotide analogues of the substrate, S‐acetylpantatheine and it's 4′‐phosphate, also possess substrate properties (Vmax= 1.5% and 15% of the maximal rate ...
A G, Rabinkov, V V, Velikodvorskaya, V M, Kopelevich, E A, Tolosa, V I, Gunar   +4 more
openaire   +2 more sources

Chemoproteomics Yields a Selective Molecular Host for Acetyl-CoA

, 2023
Chemoproteomic profiling is a powerful approach to define the selectivity of small molecules and endogenous metabolites with the human proteome. In addition to mechanistic studies, proteome specificity profiling also has the potential to identify new ...
Zachary A. Brown (16639828), Yihang Jing, Emily Megill (16639834), McKenna C. Crawford (10966446), Jordan L. Meier, Daniel S. Kantner, Carissa Grose, Whitney K. Lieberman (16639825), Nya D. Evans, Jane E. Jones (16639840), Isita Jhulki, Jordan L. Meier (1280976), Zachary A. Brown, Daniel S. Kantner (16639831), Yihang Jing (9173722), Nathaniel W. Snyder (1282137), Emily Megill, Nya D. Evans (16639837), Nathaniel W. Snyder, McKenna C. Crawford, Isita Jhulki (2855828), Carissa Grose (4774509), Whitney K. Lieberman, Jane E. Jones   +23 more
core   +2 more sources

Acetyl group coordinated progression through the catalytic cycle of an arylalkylamine N-acetyltransferase. [PDF]

PLoS ONE, 2017
The transfer of an acetyl group from acetyl-CoA to an acceptor amine is a ubiquitous biochemical transformation catalyzed by Gcn5-related N-acetyltransferases (GNATs).
Adam A Aboalroub, Ashleigh B Bachman, Ziming Zhang, Dimitra Keramisanou, David J Merkler, Ioannis Gelis   +5 more
doaj   +1 more source

Dynamic-tuning yeast storage carbohydrate improves the production of acetyl-CoA-derived chemicals

iScience, 2023
Summary: Acetyl-coenzyme A (Acetyl-CoA) and malonyl-coenzyme A (malonyl-CoA) are important precursors for producing various chemicals, and their availability affects the production of their downstream chemicals.
Chenxi Qiu, Huilin Tao, Yu Shen, Qingsheng Qi, Jin Hou   +4 more
doaj   +1 more source

Metabolic engineering for efficient supply of acetyl-CoA from different carbon sources in Escherichia coli

Microbial Cell Factories, 2019
Background Acetyl-CoA is an important metabolic intermediate and serves as an acetylation precursor for the biosynthesis of various value-added acetyl-chemicals.
Shasha Zhang, Wei Yang, Hao Chen, Bo Liu, Baixue Lin, Yong Tao   +5 more
doaj   +1 more source