Results 91 to 100 of about 70,698 (266)

Cell and Gene Therapies Manufacturing Challenges and Integrated Good Manufacturing Practices Solutions: A Lifecycle Perspective

Biotechnology and Bioengineering, EarlyView.
Lifecycle perspective on cell and gene therapy manufacturing challenges and enabling GMP solutions. ABSTRACT Cell and gene therapies (CGTs) are revolutionizing modern medicine; however, making these advanced medicines scalable and readily available to commercial manufacturers worldwide is a major challenge. The number of approved CGT products continues
Rajath Samaga, Udisha Singh, Sachin Dattram Pawar   +2 more
wiley   +1 more source

Adenoviral vectors for gene therapy

Adenoviral Vectors for Gene Therapy, Third Edition, provides detailed, comprehensive coverage of gene delivery vehicles based on the adenovirus that is emerging as an important tool in gene therapy.
San Martín, Carmen, Gallardo, José, Martínez, Marta, Condezo, Gabriela N.   +3 more
core   +1 more source

Adenoviral-mediated gene transfer to the filtering bleb in rabbits

, 2001
Purpose: To determine if genes can be transferred to fibroblasts in the filtering bleb using adenoviral vectors. Materials and Methods: Twelve New Zealand albino rabbits underwent bilateral full-thickness sclerostomies.
Skaf, Mirian [UNIFESP], Mello, Paulo Augusto de Arruda [UNIFESP], di Martino, D. S., Varma, R.   +3 more
core   +1 more source

Mechanistic Digital Model for Real‐Time Monitoring and Optimization of Adenovirus Production

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adenoviral vectors are a critical vaccine platform, with billions of vaccine doses administered during the COVID‐19 pandemic. In addition, they are promising therapeutics for cancer treatment. To advance their manufacturing, real‐time monitoring of adenovirus production is essential for process optimization, yield improvement, and product ...
Xingge Xu, Daniel Rüdiger, Jeongbin Shin, Shawninder Chahal, Amine Kamen   +4 more
wiley   +1 more source

Heterologous prime-boost vaccination drives stromal activation and adaptive immunity against SARS-CoV-2 variants

Frontiers in Immunology
Heterologous vaccination strategies have shown superior efficacy over homologous regimens in clinical studies, but the underlying immunological mechanisms remain incompletely understood.
Ji Hyang Jeon, Ji Hyang Jeon, Seongryong Kim, Seo-Yeon Kim, Kwang-Soo Shin, Bongju Park, Soojeong Chang, Chang-Yuil Kang, You-Jin Kim, Jong-Eun Park, Sungsu Youk, Sungsu Youk, Dokeun Kim, Jinah Yeo   +13 more
doaj   +1 more source

Cryo‐EM Analysis of Adenovirus Vector Reveals Purification Conditions Leading to Loss of Viral Fiber Proteins and Reduced Infectivity

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Vaccines and gene therapies require efficient delivery of genetic material to host cells. Viral vectors leverage viruses' ability to enter host cells to achieve this. Following the SARS‐CoV‐2 vaccine and novel gene therapies that utilize adenovirus vectors, there has been an increase in the demand for large‐scale production of vectors.
Betsy Widdicombe, Joseph D. Barritt, Nichakorn Pipatpadungsin, Braulio Carrillo Sanchez, Dale Stibbs, Lisa Cooper, Sarah L. Rouse, Daniel G. Bracewell   +7 more
wiley   +1 more source

Serotype‐Tailored Midstream and Downstream Operations to Advance the Manufacturing of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viruses (AAVs) are the leading vectors for in vivo gene therapy, fueling a growing demand for scalable and robust biomanufacturing processes. Yet, the impact of cell lysis conditions on the performance of midstream and downstream unit operations across serotypes remains poorly characterized.
Arianna Minzoni, Shriarjun Shastry, Eduardo Barbieri, Morgan R. Hurst, Kelvin Idanwekhai, Yuxuan Wu, Katherine Ernst, Wai‐Keen Chung, Christopher Bombeck, Saad A. Khan, Sushmita Koley, Gabriella Kiss, Oliver Rammo, Alexander Tropsha, Cristiana Boi, Michael A. Daniele, Steven Cramer, Barry Byrne, Stephen Kaminsky, Angie Snell Bennett, Stefano Menegatti   +20 more
wiley   +1 more source

Adeno‐associated virus‐mediated restoration of NaV1.1 in targeted brain regions ameliorates epilepsy in symptomatic Dravet syndrome mice

Epilepsia, EarlyView.
Abstract Objective Dravet syndrome (DS) is a developmental and epileptic encephalopathy primarily caused by haploinsufficiency of the SCN1A gene, which encodes the alpha subunit of NaV1.1 voltage‐gated sodium channel. The disease manifests with febrile and spontaneous seizures, developmental delay, cognitive impairment, and increased risk of sudden ...
Martina Mainardi, Simone Brusco, Serena Gea Giannelli, Vania Broccoli, Gaia Colasante   +4 more
wiley   +1 more source

Determination of Prevalence, Sensitivity and Specificity of Clinical Signs and Symptoms of Adenoviral Keratoconjunctivitis

مجله دانشکده پزشکی اصفهان, 2013
Background: Adenoviral keratoconjunctivitis is a prevalent form of eye infection which involves the conjunctiva and less commonly the cornea. The signs and symptoms of the disease are more often overlapped with other causes of conjunctivitis.
Masoud Mokhtari, Amir Reza Laripour Tehranfar, Sharareh Moghim, Ali Reza Dehghani, Ziba Farajzadegan, Nafiseh Sadat Hosseini, Seyyed Ali Razavinasab   +6 more
doaj  

The Use of Adenoviral Vectors and Ex Vivo Transduced Neurotransplants: Towards Promotion of Neuroregeneration

Cell Transplantation, 2000
Regeneration of injured axons following injury depends on a delicate balance between growth-promoting and growth-inhibiting factors. Overexpression of neurotrophin genes seems a promising strategy to promote regeneration.
Bas Blits, Paul A. Dijkhuizen, Wim T. J. M. C. Hermens, Lisette K. E. Van Esseveldt, Gerard J. Boer, Joost Verhaagen   +5 more
doaj   +1 more source