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Isolation of Human Adipose-Derived Stem Cells
Human adipose-derived stromal/stem cells (hASCs) are a promising source of adult stem cells used in numerous applications in regenerative medicine. We present the protocols from our laboratory for isolating and expanding hASCs. The isolation of hASCs involves the enzymatic digestion of adipose tissue and subsequent culturing of the isolated cells.Elizabeth, Montano +2 more
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Viral Transduction of Adipose-Derived Stem Cells
2010Increasing numbers of regenerative approaches now involve use of adult stem cells, like the bone marrow MSC or the adipose-derived ASC. With their ease of in vitro manipulation and successful tissue integration in vivo, the ASC makes an attractive candidate for gene delivery in vivo using viral-based gene therapy strategies.
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Adipose-Derived Stem Cells for Skin Regeneration
2010Intractable skin ulcers resulting from diabetes, ischemia and collagen diseases represent significant problems with few solutions. Cell-based therapy may hold promise in overcoming such disorders. In order to establish a suitable experimental model for the treatment of such ulcers using stem cells, this chapter describes detailed methods for: (1 ...
Hiroshi, Mizuno, Masaki, Nambu
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Adipose-Derived Stem Cells Secretome and Its Potential Application in “Stem Cell-Free Therapy”
Biomolecules, 2021Anna Sendera +1 more
exaly
Yield of human adipose-derived adult stem cells from liposuction aspirates
Cytotherapy, 2004J M Gimble
exaly

