Results 11 to 20 of about 133,679 (301)

Determination of ADP‐ribose and Poly(ADP‐ribose) by a New Radioimmunoassay [PDF]

European Journal of Biochemistry, 1978
A specific and sensitive radioimmunoassay for ADP‐ribose has been developed on the basis of the selective conversion of ADP‐ribose to 5′‐AMP by alkaline treatment. Antibodies highly specific against 5′‐AMP allowed quantification of ADP‐ribose converted to 5′‐AMP in the range of 1–40 pmol, and in the presence of large quantities of nucleic acids or 3 ...
R, Bredehorst, A M, Ferro, H, Hilz
openaire   +2 more sources

Analysis of O-Acetyl-ADP-ribose as a Target for Nudix ADP-ribose Hydrolases [PDF]

Journal of Biological Chemistry, 2002
The Sir2 family of NAD(+)-dependent histone/protein deacetylases has been implicated in a wide range of biological activities, including gene silencing, life span extension, and chromosomal stability. Recent evidence has indicated that these proteins produce a novel metabolite O-acetyl-ADP-ribose (OAADPr) during deacetylation.
Louise A, Rafty, Manning T, Schmidt, Anne-Laure, Perraud, Andrew M, Scharenberg, John M, Denu   +4 more
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The XRCC1 phosphate-binding pocket binds poly (ADP-ribose) and is required for XRCC1 function [PDF]

, 2015
Poly (ADP-ribose) is synthesized at DNA single-strand breaks and can promote the recruitment of the scaffold protein, XRCC1. However, the mechanism and importance of this process has been challenged.
Breslin, Claire, Caldecott, Keith W, Hanzlikova, Hana, Hornyak, Peter, Oliver, Antony W, Ridley, Andrew, Rulten, Stuart L   +6 more
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Chromosomal instability syndromes are sensitive to poly ADP-ribose polymerase inhibitors

Haematologica, 2008
Poly ADP-ribose polymerase inhibitors have been shown to target cells with homologous recombination DNA repair defects. We report that poly ADP-ribose polymerase inhibitors induces apoptosis in cells deficient in other key DNA repair components ...
Terry J. Gaymes, Sydney Shall, Farzin Farzaneh, Ghulam J. Mufti   +3 more
doaj   +1 more source

Cyclic ADP-Ribose and Hydrogen Peroxide Synergize with ADP-Ribose in the Activation of TRPM2 Channels [PDF]

Molecular Cell, 2005
The melastatin-related transient receptor potential channel TRPM2 is a plasma membrane Ca2+-permeable cation channel that is activated by intracellular adenosine diphosphoribose (ADPR) binding to the channel's enzymatic Nudix domain. Channel activity is also seen with nicotinamide dinucleotide (NAD+) and hydrogen peroxide (H2O2), but their mechanisms ...
Kolisek, Martin, Beck, Andreas, Fleig, Andrea, Penner, Reinhold   +3 more
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Extracellular-Added ADP-Ribose Increases Intracellular Free Ca2+ Concentration Through Ca2+ Release From Stores, but Not Through TRPM2-Mediated Ca2+ Entry, in Rat β-Cell Line RIN-5F

Journal of Pharmacological Sciences, 2006
.: Intracellular ADP-ribose is an activator of TRPM2, which is a Ca2+-permeable channel and mediates H2O2-induced cell death, in the TRPM2-expressing rat β-cell line RIN-5F.
Ishii Masakazu, Shimizu Shunichi, Hagiwara Tamio, Wajima Teruaki, Miyazaki Akira, Mori Yasuo, Kiuchi Yuji   +6 more
doaj   +1 more source

ADP-Ribose Activates the TRPM2 Channel from the Sea Anemone Nematostella vectensis Independently of the NUDT9H Domain. [PDF]

PLoS ONE, 2016
The human redox-sensitive Transient receptor potential melastatin type 2 (hTRPM2) channel contains the C-terminal Nudix hydrolase domain NUDT9H which most likely binds ADP-ribose.
Frank J P Kühn, Cornelia Kühn, Mathis Winking, Daniel C Hoffmann, Andreas Lückhoff   +4 more
doaj   +1 more source

Synthesis of Dimeric ADP-Ribose and Its Structure with Human Poly(ADP-ribose) Glycohydrolase [PDF]

Journal of the American Chemical Society, 2015
Poly(ADP-ribosyl)ation is a common post-translational modification that mediates a wide variety of cellular processes including DNA damage repair, chromatin regulation, transcription, and apoptosis. The difficulty associated with accessing poly(ADP-ribose) (PAR) in a homogeneous form has been an impediment to understanding the interactions of PAR with ...
Michael J. Lambrecht, Matthew Brichacek, Eva Barkauskaite, Antonio Ariza, Ivan Ahel, Paul J. Hergenrother   +5 more
openaire   +2 more sources

Structural and Functional Characterization of Legionella pneumophila Effector MavL

Biomolecules, 2021
Legionella pneumophila is a Gram-negative intracellular pathogen that causes Legionnaires’ disease in elderly or immunocompromised individuals. This bacterium relies on the Dot/Icm (Defective in organelle trafficking/Intracellular multiplication) Type IV
Kevin Voth, Shivani Pasricha, Ivy Yeuk Wah Chung, Rachelia R. Wibawa, Engku Nuraishah Huda E. Zainudin, Elizabeth L. Hartland, Miroslaw Cygler   +6 more
doaj   +1 more source

PARP-1 dependent recruitment of the amyotrophic lateral sclerosis-associated protein FUS/TLS to sites of oxidative DNA damage [PDF]

, 2013
Amyotrophic lateral sclerosis (ALS) is associated with progressive degeneration of motor neurons. Several of the genes associated with this disease encode proteins involved in RNA processing, including fused-in-sarcoma/translocated-in-sarcoma (FUS/TLS ...
Adamson, Ahel, Amy Rotheray, Amé, Beli, Bertolotti, Chou, Da Cruz, Das, Dormann, Dormann, Duncan A. Q. Moore, El-Khamisy, Fernando Gómez-Herreros, Gabrielle J. Grundy, Gagné, Gardiner, Gitler, Hallier, Hanson, Hicks, Hoell, Johansson, Keith W Caldecott, Kino, Kleiman, Kleiman, Kleiman, Krietsch, Kuroda, Kwiatkowski, Lagier-Tourenne, Lagier-Tourenne, Lehtiö, Mackenzie, Majid Hafezparast, Malanga, Mastrocola, Masutani, Polo, Polymenidou, Rulten, Rulten, Ryan L. Green, Schwartz, Shieh, Stuart L. Rulten, Tan, Tan, Turner, Vance, Wang, Yang, Yang   +53 more
core   +1 more source