Results 31 to 40 of about 133,679 (301)

Chromatin composition is changed by poly(ADP-ribosyl)ation during chromatin immunoprecipitation. [PDF]

PLoS ONE, 2012
Chromatin-immunoprecipitation (ChIP) employs generally a mild formaldehyde cross-linking step, which is followed by isolation of specific protein-DNA complexes and subsequent PCR testing, to analyze DNA-protein interactions.
Sascha Beneke, Kirstin Meyer, Anja Holtz, Katharina Hüttner, Alexander Bürkle   +4 more
doaj   +1 more source

The importance of Poly(ADP-Ribose) Polymerase as a sensor of unligated Okazaki fragments during DNA replication [PDF]

, 2018
Poly(ADP-ribose) is synthesized by PARP enzymes during the repair of stochastic DNA breaks. Surprisingly, however, we show that most if not all endogenous poly(ADP-ribose) is detected in normal S phase cells at sites of DNA replication. This S phase poly(
Caldecott, Keith W, Cihlarova, Zuzana, Demin, Annie A, Hanzlikova, Hana, Kalasova, Ilona, Pennicott, Lewis E   +5 more
core   +1 more source

Linking DNA repair and cell cycle progression through serine ADP-ribosylation of histones

Nature Communications, 2022
Poly(ADP-ribose)-polymerases (PARPs) are a cornerstone of the DNA damage response that promote DNA repair by modifying target proteins with ADP-ribose.
Julien Brustel, Tetsuya Muramoto, Kazuki Fumimoto, Jessica Ellins, Catherine J. Pears, Nicholas D. Lakin   +5 more
doaj   +1 more source

Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription

Scientific Reports, 2018
Macrodomains are conserved protein folds associated with ADP-ribose binding and turnover. ADP-ribosylation is a posttranslational modification catalyzed primarily by ARTD (aka PARP) enzymes in cells.
Mareike Bütepage, Christian Preisinger, Alexander von Kriegsheim, Anja Scheufen, Eva Lausberg, Jinyu Li, Ferdinand Kappes, Regina Feederle, Sabrina Ernst, Laura Eckei, Sarah Krieg, Gerhard Müller-Newen, Giulia Rossetti, Karla L. H. Feijs, Patricia Verheugd, Bernhard Lüscher   +15 more
doaj   +1 more source

Biallelic ADPRHL2 mutations in complex neuropathy affect ADP ribosylation and DNA damage response

Life Science Alliance, 2021
This work studies known and new ADPRHL2 mutations with different disease mechanisms overall indicating that loss of nuclear ARH3 alone is pathogenic via dysregulated nuclear ADP ribosylation.
Danique Beijer, Thomas Agnew, Johannes Gregor Matthias Rack, Evgeniia Prokhorova, Tine Deconinck, Berten Ceulemans, Stojan Peric, Vedrana Milic Rasic, Peter De Jonghe, Ivan Ahel, Jonathan Baets   +10 more
doaj   +1 more source

DNA repair deficiency biomarkers and the 70-gene ultra-high risk signature as predictors of veliparib/carboplatin response in the I-SPY 2 breast cancer trial. [PDF]

, 2017
Veliparib combined with carboplatin (VC) was an experimental regimen evaluated in the biomarker-rich neoadjuvant I-SPY 2 trial for breast cancer. VC showed improved efficacy in the triple negative signature.
Berry, Don, Brown-Swigart, Lamorna, Buxton, Meredith, DeMichele, Angela, Esserman, Laura, Glas, Annuska, Hirst, Gillian, Hylton, Nola, Linn, Sabine, Olopade, Olufunmilayo, Paoloni, Melissa, Petricoin, Emanuel, Rugo, Hope, Sanil, Ashish, Severson, Tesa M, Symmans, Fraser, van 't Veer, Laura, Wolf, Denise M, Wulfkuhle, Julia, Yau, Christina, Yee, Doug   +20 more
core   +2 more sources

Neurological and histological consequences induced by in vivo cerebral oxidative stress: evidence for beneficial effects of SRT1720, a sirtuin 1 activator, and sirtuin 1-mediated neuroprotective effects of poly(ADP-ribose) polymerase inhibition.

PLoS ONE, 2014
Poly(ADP-ribose)polymerase and sirtuin 1 are both NAD(+)-dependent enzymes. In vitro oxidative stress activates poly(ADP-ribose)polymerase, decreases NAD(+) level, sirtuin 1 activity and finally leads to cell death.
Cindy Gueguen, Bruno Palmier, Michel Plotkine, Catherine Marchand-Leroux, Valérie C Besson   +4 more
doaj   +1 more source

PARG suppresses tumorigenesis and downregulates genes controlling angiogenesis, inflammatory response, and immune cell recruitment

BMC Cancer, 2022
Chemokines are highly expressed in tumor microenvironment and play a critical role in all aspects of tumorigenesis, including the recruitment of tumor-promoting immune cells, activation of cancer-associated fibroblasts, angiogenesis, metastasis, and ...
Sarah Johnson, Yaroslava Karpova, Danping Guo, Atreyi Ghatak, Dmitriy A. Markov, Alexei V. Tulin   +5 more
doaj   +1 more source

Cas9 is mostly orthogonal to human systems of DNA break sensing and repair.

PLoS ONE, 2023
CRISPR/Cas9 system is а powerful gene editing tool based on the RNA-guided cleavage of target DNA. The Cas9 activity can be modulated by proteins involved in DNA damage signalling and repair due to their interaction with double- and single-strand breaks (
Ekaterina A Maltseva, Inna A Vasil'eva, Nina A Moor, Daria V Kim, Nadezhda S Dyrkheeva, Mikhail M Kutuzov, Ivan P Vokhtantsev, Lilya M Kulishova, Dmitry O Zharkov, Olga I Lavrik   +9 more
doaj   +1 more source

Computational Bioprospecting Guggulsterone against ADP Ribose Phosphatase of SARS-CoV-2

Molecules, 2022
Coronavirus Disease-2019 (COVID-19) is a highly contagious disease caused by Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2). The World Health Organization (WHO) classified the disease a as global public health hazard on 11 March 2020 ...
Mateusz Kciuk, Somdutt Mujwar, Isha Rani, Kavita Munjal, Adrianna Gielecińska, Renata Kontek, Kamal Shah   +6 more
doaj   +1 more source