Results 31 to 40 of about 738,160 (293)

Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions [PDF]

, 2017
Positive selection in the two-domain type 1 pilus adhesin FimH enhances Escherichia coli fitness in urinary tract infection (UTI). We report a comprehensive atomic-level view of FimH in two-state conformational ensembles in solution, composed of one low ...
Dodson, Karen W, Gross, Michael L, Hannan, Thomas J, Hibbing, Michael E, Holehouse, Alex S, Hultgren, Scott J, Janetka, James, Kalas, Vasilios, Pappu, Rohit V, Pinkner, Jerome S, Tolia, Niraj H, Zhang, Hao   +11 more
core   +3 more sources

Learning a Robust Local Manifold Representation for Hyperspectral Dimensionality Reduction

IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing, 2017
Local manifold learning has been successfully applied to hyperspectral dimensionality reduction in order to embed nonlinear and nonconvex manifolds in the data.
Danfeng Hong, Naoto Yokoya, Xiao Xiang Zhu   +2 more
doaj   +1 more source

Carbon nanotube decorated magnetic microspheres as an affinity matrix for biomolecules [PDF]

, 2013
Carbon nanotube (CNT) decorated magnetic microspheres were fabricated to develop a multimodal platform that utilizes non-covalent molecular interactions of CNTs to magnetically separate biomolecules.
Kolkar Mohammed, Javed Hussain Niazi, Ünal, Hayriye   +1 more
core   +1 more source

Rapid de novo discovery of peptidomimetic affinity reagents for human angiotensin converting enzyme 2

Communications Chemistry, 2022
Angiotensin converting enzyme 2 (ACE2) has been identified as a cardiovascular disease biomarker and the primary receptor utilized by SARS-CoV-2, but developing serum-stable, selective and high-affinity binders for this target is challenging.
Genwei Zhang, Joseph S. Brown, Anthony J. Quartararo, Chengxi Li, Xuyu Tan, Stephanie Hanna, Sarah Antilla, Amanda E. Cowfer, Andrei Loas, Bradley L. Pentelute   +9 more
doaj   +1 more source

Yeast Surface Display Platform for Rapid Selection of an Antibody Library via Sequential Counter Antigen Flow Cytometry

Antibodies, 2022
Yeast surface display techniques have been increasingly employed as a tool for both the discovery and affinity maturation of antibodies. In this study, we describe the use of yeast surface display for the selection and affinity maturation of antibodies ...
Bhupal Ban, Robert C. Blake, Diane A. Blake   +2 more
doaj   +1 more source

Machine Learning to Quantify In Situ Humoral Selection in Human Lupus Tubulointerstitial Inflammation

Frontiers in Immunology, 2020
In human lupus nephritis, tubulointerstitial inflammation (TII) is associated with in situ expansion of B cells expressing anti-vimentin antibodies (AVAs). The mechanism by which AVAs are selected is unclear.
Andrew J. Kinloch, Yuta Asano, Azam Mohsin, Carole Henry, Rebecca Abraham, Anthony Chang, Christine Labno, Patrick C. Wilson, Marcus R. Clark   +8 more
doaj   +1 more source

Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA) [PDF]

, 2013
The aim of this study was to identify potential ligands of PSMA suitable for further development as novel PSMA-targeted peptides using phage display technology. The human PSMA protein was immobilized as a target followed by incubation with a 15-mer phage
A Ghosh, AC Pawlyk, AM Quinn, CW Diehnelt, D Hessels, D Shen, DB Sodee, Duanwen Shen, E Basch, F Yi, Fei Xie, GA Lazar, H Liu, H Liu, H-S Lim, HD Agnew, IM Thompson, Irina U. Agoulnik, J Yu, JA Arter, JR Mesters, JR Newton, KA Noren, MI Davis, MI Milowsky, MJ Morris, MJ Morris, MJ Roobol, NH Bander, RK Nam, S Aggarwal, S Kim, S Minner, SE Lupold, SM Hillier, SR Banerjee, SR Banerjee, SR Banerjee, SR Banerjee, SS Chang, SZ Li, TJ Wigle, V Humblet, W. Barry Edwards, Y Chen, Y Chen, Y Chen, Z Xiao, ZY Zhu   +48 more
core   +7 more sources

Hierarchy and assortativity as new tools for affinity investigation: the case of the TBA aptamer-ligand complex

, 2017
Aptamers are single stranded DNA, RNA or peptide sequences having the ability to bind a variety of specific targets (proteins, molecules as well as ions).
Alfinito, E., Cataldo, R., Reggiani, L.
core   +1 more source

Models of self-peptide sampling by developing T cells identify candidate mechanisms of thymic selection [PDF]

, 2013
Conventional and regulatory T cells develop in the thymus where they are exposed to samples of self-peptide MHC (pMHC) ligands. This probabilistic process selects for cells within a range of responsiveness that allows the detection of foreign antigen ...
Bains, Iren, Seddon, Benedict, van Santen, Hisse M., Yates, Andrew J.   +3 more
core   +1 more source

Direct Dynamic Protein-Affinity Selection Mass-Spectrometry [PDF]

Chromatographia, 2010
A new methodology is described enabling the affinity screening of potential ligands towards the human estrogen receptor alpha ligand binding domain (ERalpha-LBD). In-solution incubation is performed of the analyte and the His-tagged ERalpha-LBD. The bound complex is immobilized on a nickel-loaded protein-affinity selection column, where after the ...
Jonker, N., Lingeman, H., Irth, H.
openaire   +2 more sources