Results 81 to 90 of about 8,123 (210)

Testing multiplexed anti-ASFV CRISPR-Cas9 in reducing African swine fever virus

Microbiology Spectrum
ABSTRACT African swine fever (ASF) is a highly fatal viral disease that poses a significant threat to domestic pigs and wild boars globally. In our study, we aimed to explore the potential of a multiplexed CRISPR-Cas system in suppressing ASFV replication and infection.
Zezhong Zheng, Lei Xu, Yangbin Gao, Hongwei Dou, Yixuan Zhou, Xu Feng, Xiangjun He, Zhen Tian, Lingling Song, Guolong Mo, Jiapan Hu, Hongye Zhao, Hongjiang Wei, George M. Church, Luhan Yang   +14 more
openaire   +3 more sources

Evaluation of the Function of the ASFV KP177R Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence

Viruses, 2021
African swine fever virus (ASFV) causes a devastating disease of swine that has caused outbreaks in Central Europe since 2007, spreading into Asia in 2018.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca   +7 more
doaj   +1 more source

African swine fever virus assembles a single membrane derived from rupture of the endoplasmic reticulum [PDF]

, 2016
Collective evidence argues that two members of the nucleocytoplasmic large DNA viruses (NCLDVs) acquire their membrane from open membrane intermediates, postulated to be derived from membrane rupture.
Andrés, Germán, Hoppe, S., Kolovou, Androniki, Krijnse Locker, J., Salas, María Luisa, Suárez, Cristina, Walther, P.   +6 more
core   +1 more source

Advances in Double‐Stranded DNA Targeting Technologies

Exploration, Volume 6, Issue 1, February 2026.
Double‐stranded DNA (dsDNA) plays a crucial role in genetic information storage and disease management, but its inherent stability limits access to internal bases. To overcome this challenge, various high‐specificity molecular targeting technologies have been developed to destabilize the DNA structure.
Zuhao Shen, Yiqun Liu, Yingjie Hao, Yifan Bo, Xiaochuan Dai, Shihui Wang, Tian Xia, Xin Su, Huiyu Liu   +8 more
wiley   +1 more source

Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples

Viruses, 2020
African swine fever virus (ASFV) is causing outbreaks both in domestic pigs and wild boar in Europe and Asia. In 2018, the largest pig producing country, China, reported its first outbreak of African swine fever (ASF). Since then, the disease has quickly
Ayushi Rai, Sarah Pruitt, Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ediane Silva, Lauro Velazquez-Salinas, Consuelo Carrillo, Manuel V. Borca, Douglas P. Gladue   +8 more
doaj   +1 more source

Pyroptosis in Peripheral Neuropathy: From Molecular Mechanisms to Therapeutic Targeting

CNS Neuroscience &Therapeutics, Volume 32, Issue 1, January 2026.
Pyroptosis is a key inflammatory cell death pathway in peripheral neuropathy, with opposing roles: it perpetuates neuropathic pain but can be induced to kill neuroblastoma cells. This review consolidates mechanistic understanding and therapeutic strategies, arguing for a shift from disease‐centric to target‐centric treatment approaches to improve ...
Jinhuan Wei, Mingyue Liuyuan, Zhixin Ye, Xueying Wang, Xueli Zhou, Yifan Shan, Cheng He, Chunting Zhu, Chicheng Zhou, Jingyin Bao, Yonghui Zhang, Gang Chen   +11 more
wiley   +1 more source

The CP123L protein of African swine fever virus is a membrane-associated, palmitoylated protein required for viral replication

Journal of Virology
African swine fever (ASF) is a highly contagious and often lethal disease caused by African swine fever virus (ASFV) in pigs. Protein palmitoylation is a prevalent posttranslational lipid modification that can modulate viral replication.
Xiangyu Guan, Tao Wang, Yuxuan Gao, Huanjie Zhai, Fengwei Jiang, Qinghe Hou, Xiaoke Yang, Hongxia Wu, Lian-Feng Li, Yuzi Luo, Su Li, Yuan Sun, Hua-Ji Qiu, Yongfeng Li   +13 more
doaj   +1 more source

Multifaceted Small Molecules as Enzyme Modulators: Cases of Drug Discovery/Repurposing Illustrating Nature′s Pragmatism

BioMed Research International, Volume 2026, Issue 1, 2026.
The cross‐reactivity of substrates, modulators with other enzymes significantly reduces/prevents our ability to design such highly specific, that is, “one warhead–one target”, modulators. On the other hand, the potential “impasse” fuels repurposing of already developed drugs.
Monika I. Konaklieva, Balbina J. Plotkin, Nahit Gencer   +2 more
wiley   +1 more source

Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication

mBio
African swine fever (ASF), caused by the African swine fever virus (ASFV), is a highly contagious disease that affects pigs, resulting in substantial economic losses in the global pig industry.
Danyang Zhang, Baohong Liu, Huanan Liu, Ruoqing Mao, Weijun Cao, Xiangle Zhang, Fayu Yang, Yichao Wang, Chaochao Shen, Shilei Zhang, Zixiang Zhu, Haixue Zheng   +11 more
doaj   +1 more source

Evaluation in Swine of a Recombinant African Swine Fever Virus Lacking the MGF-360-1L Gene

Viruses, 2020
The African swine fever (ASF) pandemic is currently affecting pigs throughout Eurasia, resulting in significant swine production losses. The causative agent, ASF virus (ASFV), is a large, structurally complex virus with a genome encoding more than 160 ...
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ayushi Rai, Sarah Pruitt, Ediane Silva, Lauro Velazquez-Salinas, James Zhu, Douglas P. Gladue, Manuel V. Borca   +8 more
doaj   +1 more source