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International Journal of Biological Macromolecules
Alginate is mainly a linear polysaccharide composed of randomly arranged β-D-mannuronic acid and α-L-guluronic acid linked by α, β-(1,4)-glycosidic bonds. Alginate lyases degrade alginate mainly adopting a β-elimination mechanism, breaking the glycosidic bonds between the monomers and forming a double bond between the C4 and C5 sugar rings to produce ...
Chao Qiu, Jie Long
exaly +3 more sources
Alginate is mainly a linear polysaccharide composed of randomly arranged β-D-mannuronic acid and α-L-guluronic acid linked by α, β-(1,4)-glycosidic bonds. Alginate lyases degrade alginate mainly adopting a β-elimination mechanism, breaking the glycosidic bonds between the monomers and forming a double bond between the C4 and C5 sugar rings to produce ...
Chao Qiu, Jie Long
exaly +3 more sources
Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress
Alginate oligosaccharides (AOS) prepared from degradation of alginate is a potent plant elicitor. Hydroponic experiments were carried out to investigate the mechanism of AOS on improving Triticum aestivum L resistant ability to drought stress.
Heng Yin, Xiaomin Shi, Xiao-Ming Zhao
exaly +2 more sources
Preparation and potential applications of alginate oligosaccharides
Critical Reviews in Food Science and Nutrition, 2022Alginate, a linear polymer consisting of β-D-mannuronic acid (M) and α-L-guluronic acid (G) with 1,4-glycosidic linkages and comprising 40% of the dry weight of algae, possesses various applications in the food and nutraceutical industries. However, the potential applications of alginate are restricted in some fields because of its low water solubility
Decheng Bi, Xu Yang, Jun Lu, Xu Xu
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International Journal of Biological Macromolecules, 2020
Alginate lyases are important enzymes to prepare alginate oligosaccharides for industrial applications and elucidating the degradation pattern of alginate lyases is essential for expanding their applications. Herein, we cloned and expressed AlyPL6, a novel member of polysaccharide lyase family-6 (PL6) with high activity from Pedobacter hainanensis NJ ...
Qian, Li +5 more
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Alginate lyases are important enzymes to prepare alginate oligosaccharides for industrial applications and elucidating the degradation pattern of alginate lyases is essential for expanding their applications. Herein, we cloned and expressed AlyPL6, a novel member of polysaccharide lyase family-6 (PL6) with high activity from Pedobacter hainanensis NJ ...
Qian, Li +5 more
openaire +2 more sources
Preparation of alginate oligosaccharides and their biological activities in plants: A review
Carbohydrate Research, 2020Alginate oligosaccharide (AOS) is the degradation product of alginates extracted from brown algae. As a multifunctional oligomer, it has attracted widespread attention in plant research. Different methods of preparation generate AOS possessing diverse structural properties, and result in differences in AOS activity.
Chunguang, Zhang +4 more
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Production of Oligosaccharide from Alginate Using Pseudoalteromonas agarovorans
Applied Biochemistry and Biotechnology, 2009A marine bacterium was isolated from seawater near the Korean south coast for efficient saccharification from alginate. Based on 16S rDNA sequence, the isolated strain was identified as Pseudoalteromonas agarovorans. Various environmental factors affecting saccharification of alginate using P. agarovorans CHO-12 have been investigated in flask cultures.
DuBok, Choi +3 more
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Critical Reviews in Food Science and Nutrition, 2021
Alginates are linear polymers comprising 40% of the dry weight of algae possess various applications in food and biomedical industries. Alginate oligosaccharides (AOS), a degradation product of alginate, is now gaining much attention for their beneficial role in food, pharmaceutical and agricultural industries.
Chunhua Zhang +7 more
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Alginates are linear polymers comprising 40% of the dry weight of algae possess various applications in food and biomedical industries. Alginate oligosaccharides (AOS), a degradation product of alginate, is now gaining much attention for their beneficial role in food, pharmaceutical and agricultural industries.
Chunhua Zhang +7 more
openaire +2 more sources
Carbohydrate Research, 2019
This study presents a quick, simple and accurate method to calculate the degree of polymerization (DP) of alginate oligosaccharides (AOS) and low molecular weight alginates from the concentration of reducing sugar determined by 3,5-dinitrosalicylic acid (DNS) assay. 1H NMR spectroscopy, mass spectroscopy (MS) and certified standards were used to verify
Ming, Sun +4 more
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This study presents a quick, simple and accurate method to calculate the degree of polymerization (DP) of alginate oligosaccharides (AOS) and low molecular weight alginates from the concentration of reducing sugar determined by 3,5-dinitrosalicylic acid (DNS) assay. 1H NMR spectroscopy, mass spectroscopy (MS) and certified standards were used to verify
Ming, Sun +4 more
openaire +2 more sources
Food Chemistry
To achieve the cost-effective alginate oligosaccharides production, we have developed a novel rational design strategy that optimized capture behavior, orientation movement and hydrogen bond interaction of substrate in alginate lyase ALYI1. This approach led to S56D and G258Q advantageous variants balancing the trade-off challenge, particularly S56D ...
Jin-Ping, Huang +9 more
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To achieve the cost-effective alginate oligosaccharides production, we have developed a novel rational design strategy that optimized capture behavior, orientation movement and hydrogen bond interaction of substrate in alginate lyase ALYI1. This approach led to S56D and G258Q advantageous variants balancing the trade-off challenge, particularly S56D ...
Jin-Ping, Huang +9 more
openaire +2 more sources
Design, synthesis and biological evaluation of alginate oligosaccharide derivatives
Bioorganic & Medicinal Chemistry LettersTo discover new potential drug for acute kidney injury (AKI), a series of novel alginate oligosaccharide derivatives were synthesized and characterized by the spectroscopic analysis. By using the controlled experimental method, the target compounds were evaluated preliminarily for therapeutic activity in AKI. The results demonstrated that compounds 2a,
Wenkang Guo +5 more
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