Results 21 to 30 of about 3,982 (151)

The Shu complex prevents mutagenesis and cytotoxicity of single-strand specific alkylation lesions

eLife, 2021
Three-methyl cytosine (3meC) are toxic DNA lesions, blocking base pairing. Bacteria and humans express members of the AlkB enzymes family, which directly remove 3meC. However, other organisms, including budding yeast, lack this class of enzymes.
Braulio Bonilla, Alexander J Brown, Sarah R Hengel, Kyle S Rapchak, Debra Mitchell, Catherine A Pressimone, Adeola A Fagunloye, Thong T Luong, Reagan A Russell, Rudri K Vyas, Tony M Mertz, Hani S Zaher, Nima Mosammaparast, Ewa P Malc, Piotr A Mieczkowski, Steven A Roberts, Kara A Bernstein   +16 more
doaj   +1 more source

Transient kinetic analysis of oxidative dealkylation by the direct reversal DNA repair enzyme AlkB [PDF]

Journal of Biological Chemistry, 2020
AlkB is a bacterial Fe(II)- and 2-oxoglutarate-dependent dioxygenase that repairs a wide range of alkylated nucleobases in DNA and RNA as part of the adaptive response to exogenous nucleic acid-alkylating agents. Although there has been longstanding interest in the structure and specificity of Escherichia coli AlkB and its homologs, difficulties in ...
Michael R. Baldwin, Suzanne J. Admiraal, Patrick J. O'Brien   +2 more
openaire   +2 more sources

Activity of alkanmonooxygenase alkB gene in strains of hydrocarbon-oxidizing bacteria isolated from petroleum products

Вавиловский журнал генетики и селекции, 2022
Alkanmonooxygenase enzymes AlkB and Cyp153 are responsible for the aerobic degradation of n-alkanes of petroleum and petroleum products. To prove the usage of n-alkanes from oil and petroleum products by hydrocarbon- oxidizing bacteria isolated from ...
T. N. Shapiro, N. A. Manucharova, E. S. Lobakova   +2 more
doaj   +1 more source

Dynamic states of the DNA repair enzyme AlkB regulate product release [PDF]

The EMBO Reports, 2008
The 2-oxoglutarate (2OG)- and Fe(2+)-dependent dioxygenase AlkB couples the demethylation of modified DNA to the decarboxylation of 2OG. Extensive crystallographic analyses have shown no evidence of significant structural differences between complexes binding either 2OG or succinate.
Bleijlevens, B, Shivarattan, T, Flashman, E, Yang, Y, Simpson, P, Koivisto, P, Sedgwick, B, Schofield, C, Matthews, S   +8 more
openaire   +3 more sources

Schizosaccharomyces pombe Ofd2 is a nuclear 2-oxoglutarate and iron dependent dioxygenase interacting with histones. [PDF]

PLoS ONE, 2011
2-Oxoglutarate (2OG) dependent dioxygenases are ubiquitous iron containing enzymes that couple substrate oxidation to the conversion of 2OG to succinate and carbon dioxide.
Hanne Korvald, Anne Margrethe Mølstad Moe, F Henning Cederkvist, Bernd Thiede, Jon K Laerdahl, Magnar Bjørås, Ingrun Alseth   +6 more
doaj   +1 more source

Human ALKBH6 Is Required for Maintenance of Genomic Stability and Promoting Cell Survival During Exposure of Alkylating Agents in Pancreatic Cancer

Frontiers in Genetics, 2021
Alpha-ketoglutarate-dependent dioxygenase (ALKBH) is a DNA repair gene involved in the repair of alkylating DNA damage. There are nine types of ALKBH (ALKBH1-8 and FTO) identified in humans.
Shengyuan Zhao, Rodan Devega, Aaliyah Francois, Dawit Kidane   +3 more
doaj   +1 more source

Human ALKBH4 interacts with proteins associated with transcription. [PDF]

PLoS ONE, 2012
The Fe(II)- and 2-oxoglutarate (2OG)-dependent dioxygenase AlkB from E. coli is a demethylase which repairs alkyl lesions in DNA, as well as RNA, through a direct reversal mechanism. Humans possess nine AlkB homologs (ALKBH1-8 and FTO). ALKBH2 and ALKBH3
Linn G Bjørnstad, Trine J Meza, Marit Otterlei, Solveig M Olafsrud, Leonardo A Meza-Zepeda, Pål Ø Falnes   +5 more
doaj   +1 more source

Transcriptomic analyses reveal increased expression of dioxygenases, monooxygenases, and other metabolizing enzymes involved in anthracene degradation in the marine alga Ulva lactuca

Frontiers in Plant Science, 2022
To analyze the mechanisms involved in anthracene (ANT) degradation in the marine alga Ulva lactuca, total RNA was obtained from the alga cultivated without ANT and with 5 μM of ANT for 24 h, and transcriptomic analyses were performed.
Alberto González, Héctor Osorio, Stephanie Romero, Patricia Méndez, Muriel Sepúlveda, Daniel Laporte, Marlen Gutierrez-Cutiño, Rocío Santander, Eduardo Castro-Nallar, Eduardo Castro-Nallar, Alejandra Moenne   +10 more
doaj   +1 more source

Enzymological and structural studies of the mechanism of promiscuous substrate recognition by the oxidative DNA repair enzyme AlkB [PDF]

Proceedings of the National Academy of Sciences, 2009
Promiscuous substrate recognition, the ability to catalyze transformations of chemically diverse compounds, is an evolutionarily advantageous, but poorly understood phenomenon. The promiscuity of DNA repair enzymes is particularly important, because it enables diverse kinds of damage to different nucleotide bases to be repaired in ...
Bomina, Yu, John F, Hunt
openaire   +2 more sources

Structural basis for substrate discrimination byE. colirepair enzyme, AlkB

RSC Advances, 2018
Positive charge on methylated nucleotides is a prime criterion for substrate recognition byE. coliAlkB.
Namrata Jayanth, Nirmala Ogirala, Anil Yadav, Mrinalini Puranik   +3 more
openaire   +3 more sources