Results 1 to 10 of about 63,430 (242)

Allosteric regulation of kinase activity in living cells. [PDF]

Elife, 2023
The dysregulation of protein kinases is associated with multiple diseases due to the kinases' involvement in a variety of cell signaling pathways. Manipulating protein kinase function, by controlling the active site, is a promising therapeutic and investigative strategy to mitigate and study diseases.
Godbole SS, Dokholyan NV.
europepmc   +5 more sources

Allosteric regulation of kinase activity. [PDF]

Elife
The articles in this special issue highlight how modern cellular, biochemical, biophysical and computational techniques are allowing deeper and more detailed studies of allosteric kinase regulation.
Andreotti AH, Dötsch V.
europepmc   +3 more sources

Filamentation modulates allosteric regulation of PRPS. [PDF]

Elife, 2022
Phosphoribosyl pyrophosphate (PRPP) is a key intermediate in the biosynthesis of purine and pyrimidine nucleotides, histidine, tryptophan, and cofactors NAD and NADP. Abnormal regulation of PRPP synthase (PRPS) is associated with human disorders, including Arts syndrome, retinal dystrophy, and gouty arthritis. Recent studies have demonstrated that PRPS
Hu HH, Lu GM, Chang CC, Li Y, Zhong J, Guo CJ, Zhou X, Yin B, Zhang T, Liu JL.   +9 more
europepmc   +4 more sources

The structure and allosteric regulation of glutamate dehydrogenase [PDF]

Neurochemistry International, 2010
Glutamate dehydrogenase (GDH) has been extensively studied for more than 50 years. Of particular interest is the fact that, while considered by most to be a 'housekeeping' enzyme, the animal form of GDH is heavily regulated by a wide array of allosteric effectors and exhibits extensive inter-subunit communication.
Ming Li, Changhong Li, Aron Allen, Charles A. Stanley, Thomas J. Smith   +4 more
openalex   +4 more sources

The Allosteric Regulation of Pyruvate Kinase [PDF]

Journal of Biological Chemistry, 2000
Pyruvate kinase (PK) is critical for the regulation of the glycolytic pathway. The regulatory properties of Escherichia coli were investigated by mutating six charged residues involved in interdomain salt bridges (Arg(271), Arg(292), Asp(297), and Lys(413)) and in the binding of the allosteric activator (Lys(382) and Arg(431)).
VALENTINI, GIOVANNA, CHIARELLI, LAURENT, SPERANZA, MARIA LUISA, GALIZZI, ALESSANDRO, MATTEVI, ANDREA, FORTIN R   +5 more
openaire   +5 more sources

The allosteric regulation of pyruvate kinase [PDF]

FEBS Letters, 1996
Crystallographic and mutagenesis studies have unravelled the general features of the allosteric transition mechanism in pyruvate kinase. The enzyme displays a dramatic conformational change in going from the T‐ to the R‐state. All three domains forming each subunit of the tetrameric enzyme undergo simultaneous and concerted rotations, in such a way ...
MATTEVI, ANDREA, VALENTINI, GIOVANNA, BOLOGNESI M.   +2 more
openaire   +4 more sources

Modes of allosteric regulation of the ubiquitination machinery [PDF]

Current Opinion in Structural Biology, 2020
Ubiquitination is a post-translational modification crucial for cellular signaling. A diverse range of enzymes constitute the machinery that mediates attachment of ubiquitin onto target proteins. This diversity allows the targeting of various proteins in a highly regulated fashion.
Rennie, Martin L., Chaugule, Viduth K., Walden, Helen   +2 more
openaire   +3 more sources

Design of an allosterically regulated retroaldolase [PDF]

Protein Science, 2015
AbstractWe employed a minimalist approach for design of an allosterically controlled retroaldolase. Introduction of a single lysine residue into the nonenzymatic protein calmodulin led to a 15,000‐fold increase in the second order rate constant for retroaldol reaction with methodol as a substrate.
Elizabeth A. Raymond, Yurii S. Moroz, Ivan V. Korendovych, Korrie L. Mack, Jennifer H. Yoon, Olesia V. Moroz   +5 more
openaire   +3 more sources

Allosteres to regulate neurotransmitter sulfonation [PDF]

Journal of Biological Chemistry, 2019
Catecholamine neurotransmitter levels in the synapses of the brain shape human disposition-cognitive flexibility, aggression, depression, and reward seeking-and manipulating these levels is a major objective of the pharmaceutical industry. Certain neurotransmitters are extensively sulfonated and inactivated by human sulfotransferase 1A3 (SULT1A3).
Kristie Darrah, Mary Cacace, Alexander Deiters, Ting Wang, Ian Cook, Thomas S. Leyh   +5 more
openaire   +3 more sources

Allosteric Regulation of Human Plastins [PDF]

, 2021
ABSTRACTPlastins/fimbrins are conserved actin-bundling proteins contributing to motility, cytokinesis, and other cellular processes by organizing actin assemblies of strikingly different geometries as in aligned bundles and branched networks. We propose that this unique ability stems from an allosteric communication between plastins’ two actin-binding ...
Christopher L. Schwebach, Elena Kudryashova, Richa Agrawal, Weili Zheng, Edward H. Egelman, Dmitri S. Kudryashov   +5 more
openaire   +2 more sources