Results 11 to 20 of about 19,917 (239)
Light-regulated allosteric switch enables temporal and subcellular control of enzyme activity
eLife, 2020 Engineered allosteric regulation of protein activity provides significant advantages for the development of robust and broadly applicable tools. However, the application of allosteric switches in optogenetics has been scarce and suffers from critical ...
Mark Shaaya +14 more
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Frontiers in Bioengineering and Biotechnology, 2015 Modeling metabolism is fundamental for cell factory design. Central carbon metabolism (CCM) is particularly important as it provides the energy and precursors for other biological processes.
Daniel eMachado +2 more
doaj +1 more source
Rational engineering of enzyme allosteric regulation through sequence evolution analysis.
PLoS Computational Biology, 2012 Control of enzyme allosteric regulation is required to drive metabolic flux toward desired levels. Although the three-dimensional (3D) structures of many enzyme-ligand complexes are available, it is still difficult to rationally engineer an ...
Jae-Seong Yang +4 more
doaj +1 more source
Analysis &Sensing, Volume 3, Issue 1, January 2023., 2023 Lanthanide complexes are ideal donors for FRET (Förster resonance energy transfer) assays with a variety of applications from easy‐to‐use quantitation of proteins to sophisticated experiments giving real‐time information on the movement of proteins. This Review article introduces the principles of using such assays before surveying the full scope of ...
Simon Wheeler, Stephen J. Butler
wiley +1 more source
Functional selectivity of insulin receptor revealed by aptamer-trapped receptor structures
Nature Communications, 2022 DNA aptamers can activate insulin receptor as selective agonists or positive allosteric modulators. Here, authors determine structures of the insulin receptor bound to aptamers and provide a basis for the selective activation or allosteric regulation of ...
Junhong Kim +8 more
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Prediction and confirmation of a switch-like region within the N-terminal domain of hSIRT1
Biochemistry and Biophysics Reports, 2022 Many proteins display conformational changes resulting from allosteric regulation. Often only a few residues are crucial in conveying these structural and functional allosteric changes.
Angelina T. Huynh +10 more
doaj
Binding leverage as a molecular basis for allosteric regulation.
PLoS Computational Biology, 2011 Allosteric regulation involves conformational transitions or fluctuations between a few closely related states, caused by the binding of effector molecules.
Simon Mitternacht, Igor N Berezovsky
doaj +1 more source
Information propagation in time through allosteric signaling [PDF]
Phys. Rev. Research 2, 023367 (2020), 2019 Naively, one expects the information communicated by an enzyme downstream within a signaling network, in which the enzyme is embedded, to grow monotonically with the enzyme's rate of product formation. However, here we observe that this does not necessarily hold true for allosterically regulated enzymes, often observed in signaling networks.
arxiv +1 more source
Nature Communications, 2021 The allosteric regulation of the bienzyme complex imidazole glycerol phosphate synthase (HisFH) remains to be elucidated. Here, the authors provide structural insights into the dynamic allosteric mechanism by which ligand binding to the cyclase and ...
Jan Philip Wurm +6 more
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Prediction of allosteric sites and mediating interactions through bond-to-bond propensities [PDF]
Nature Communications 7, Article number: 12477 (2016), 2016 Allosteric regulation is central to many biochemical processes. Allosteric sites provide a target to fine-tune protein activity, yet we lack computational methods to predict them. Here, we present an efficient graph-theoretical approach for identifying allosteric sites and the mediating interactions that connect them to the active site.
arxiv +1 more source